Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,340,238	(T)5→4	coding (284/2268 nt)	adiA ←	arginine decarboxylase, degradative

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,340,234	0	T	.	97.8%	185.9 / ‑1.9	46	coding (288/2268 nt)	adiA	arginine decarboxylase, degradative
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base . (19/26);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTT  >  NC_000913/4340099‑4340367                                                                                                                                        |                                                                                                                                      tGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCttttc                                                                                                                                   >  1:933222/1‑139 (MQ=255)   gcggcAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCttttccc                                                                                                                                 <  2:399838/140‑4 (MQ=255)      gcAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGAt                                                                                                                             >  2:82628/1‑140 (MQ=255)      gcAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGAt                                                                                                                             <  1:721334/140‑1 (MQ=255)      gcAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGAt                                                                                                                             <  2:29894/140‑1 (MQ=255)      gcAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGAt                                                                                                                             <  2:305781/140‑1 (MQ=255)           aGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGG‑GTTTTCCCGATCGccc                                                                                                                        >  1:525551/1‑140 (MQ=255)                           gggTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCa                                                                                                        <  2:525551/140‑1 (MQ=255)                             gTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTTGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACg                                                                                                      >  2:593915/1‑140 (MQ=255)                                 tCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGtttt                                                                                                  >  1:111457/1‑140 (MQ=255)                                      gCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTgg                                                                                             <  1:203272/140‑1 (MQ=255)                                      gCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTgg                                                                                             <  1:198915/140‑1 (MQ=255)                                        aaCGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGgcg                                                                                           <  2:10806/140‑1 (MQ=255)                                        aaCGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGgcg                                                                                           >  2:82334/1‑140 (MQ=255)                                          cGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCt                                                                                         >  1:1065421/1‑140 (MQ=255)                                           ggCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCAAGCGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTc                                                                                        <  1:942084/140‑1 (MQ=255)                                               cgTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGa                                                                                    <  1:804322/140‑1 (MQ=255)                                                   cGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCt                                                                                <  2:462744/140‑1 (MQ=255)                                                   cGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCt                                                                                <  2:933222/140‑1 (MQ=255)                                                     gCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTa                                                                              <  2:111457/140‑1 (MQ=255)                                                          aaaGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGAt                                                                         <  1:174849/140‑1 (MQ=255)                                                             gTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCaa                                                                      <  1:82628/140‑1 (MQ=255)                                                                  cggTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTc                                                                 >  1:1025552/1‑140 (MQ=255)                                                                          tCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGttt                                                         >  1:308480/1‑140 (MQ=255)                                                                              gAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGAt                                                     <  2:1025552/140‑1 (MQ=255)                                                                                 tCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGtt                                                  >  1:308214/1‑140 (MQ=255)                                                                                 tCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGtt                                                  <  2:1065421/140‑1 (MQ=255)                                                                                 tCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGtt                                                  >  2:596888/1‑140 (MQ=255)                                                                                    aGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGt                                               >  1:450993/1‑140 (MQ=255)                                                                                         aaTTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGa                                          <  1:82334/140‑1 (MQ=255)                                                                                           ttCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATg                                        >  1:1050231/1‑140 (MQ=255)                                                                                                     aGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGa                              <  2:1050231/140‑1 (MQ=255)                                                                                                            gcagGTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGa                       <  2:308480/140‑1 (MQ=255)                                                                                                                gTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACAt                   >  2:636113/1‑140 (MQ=255)                                                                                                                gTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAACTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGa                              <  1:730621/129‑1 (MQ=255)                                                                                                                gTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAACTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGa                              >  2:730621/1‑129 (MQ=255)                                                                                                                gTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGACGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACAt                   >  2:954471/1‑140 (MQ=255)                                                                                                                gTCGCGATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTATGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACAt                   >  1:249552/1‑140 (MQ=255)                                                                                                                    cgATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAgg               >  2:976676/1‑140 (MQ=255)                                                                                                                    cgATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAgg               <  1:954471/140‑1 (MQ=255)                                                                                                                    cgATCCATTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAgg               <  1:635791/140‑1 (MQ=255)                                                                                                                          aTTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCa         <  2:14620/140‑1 (MQ=255)                                                                                                                          aTTGCGGCGAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCa         <  1:976676/140‑1 (MQ=255)                                                                                                                                 cgAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCt   >  2:499251/1‑140 (MQ=255)                                                                                                                                 cgAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCtt  <  2:308214/140‑1 (MQ=255)                                                                                                                                 cgAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCtt  >  2:171039/1‑140 (MQ=255)                                                                                                                                 cgAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCtt  >  1:916537/1‑140 (MQ=255)                                                                                                                                 cgAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCtt  <  1:636113/140‑1 (MQ=255)                                                                                                                                 cgAGGGC‑TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCtt  >  1:531444/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      TGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTT  >  NC_000913/4340099‑4340367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑