Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,285,180	(G)5→6	coding (496/804 nt)	agaC →	galactosamine‑specific PTS enzyme IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,285,175	1	.	G	100.0%	185.1 / NA	52	Q164R (CAG→CGG)	agaC	galactosamine‑specific PTS enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (26/26);  total (26/26)

CATTATTTATGACCAAAGCCGATAAATGCGCGAAAGAGGCGGATACGGCAGCGTTTTCCCGGCTTAACTGGACAACGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑A‑GGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAGCGCAATATATCCCTT  >  NC_000913/3285040‑3285309                                                                                                                                          |                                                                                                                                       cATTATTTATGACCAAAGCCGATAAATGCGCGAAAGAGGCGGATACGGCAGCGTTTTCCCGGCTTAACTGGACAACGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑A‑gggg                                                                                                                                    <  2:519491/140‑1 (MQ=255)   ttatttatGACCAAAGCCGATAAATGCGCGAAAGAGGCGGATACGGCAGCGTTTTCCCGGCTTAACTGGACAACAATGCTCATCGTCGCCTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑A‑gggggg                                                                                                                                  >  1:145185/1‑139 (MQ=255)     atttatGACCAAAGCCGATAAATGCGCGAAAGAGGCGGATACGGCAGCGTTTTCCCGGCTTAACTGGACAACGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑A‑ggggggcg                                                                                                                                <  2:714881/140‑4 (MQ=255)             ccAAAGCCGATAAATGCGCGAAAGAGGCGGATACGGCAGCGTTTTCCCGGCTTAACTGGACAACGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGgc                                                                                                                         <  1:714533/140‑1 (MQ=255)              cAAAGCCGATAAATGCGCGAAAGAGGCGGATACGGCAGCGTTTTCCCGGCTTAACTGGACAACGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGgcg                                                                                                                        >  2:886680/1‑140 (MQ=255)              cAAAGCCGATAAATGCGCGAAAGAGGCGGATACGGCAGCGTTTTCCCGGCTTAACTGGACAACGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTACTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGgcg                                                                                                                        <  1:520703/140‑1 (MQ=255)                aaGCCGATAAATGCGCGAAAGAGGCGGATACGGCAGCGTTTTCCCGGCTTAACTGGACAACGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCt                                                                                                                      >  1:125113/1‑140 (MQ=255)                aaGCCGATAAATGCGCGAAAGAGGCGGATACGGCAGCGTTTTCCCGGCTTAACTGGACAACGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCt                                                                                                                      >  2:49984/1‑140 (MQ=255)                              gcgAAAGAGGCGGATACGGCAGCGTTTTCCCGGCTTAACTGGACAACGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGc                                                                                                        >  1:842478/1‑140 (MQ=255)                               cgAAAGAGGCGGATACGGCAGCGTTTTCCCGGCTTAACTGGACAACGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGcc                                                                                                       >  1:600979/1‑140 (MQ=255)                                 aaaGAGGCGGATACGGCAGCGTTTTCCCGGCTTAACTGGACAACGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCg                                                                                                     <  1:109216/140‑1 (MQ=255)                                 aaaGAGGCGGATACGGCAGCGTTTTCCCGGCTTAACTGGACAACGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCg                                                                                                     <  2:93123/140‑1 (MQ=255)                                       gCGGATACGGCAGCGTTTTCCCGGCTTAACTGGACAACGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGc                                                                                               <  2:125113/140‑1 (MQ=255)                                               ggCAGCGTTTTCCCGGCTTAACTGGACAACGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCAc                                                                                       >  1:1037818/1‑140 (MQ=255)                                                 cAGCGTTTTCCCGGCTTAACTGGACAACGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACgg                                                                                     >  2:675789/1‑140 (MQ=255)                                                  aGCGTTTTCCCGGCTTAACTGGACAACGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGc                                                                                    <  2:842478/140‑1 (MQ=255)                                                             ggCTTAACTGGACAACGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGtggc                                                                         <  1:825053/140‑1 (MQ=255)                                                                         cAACGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTct                                                             >  2:714882/1‑140 (MQ=255)                                                                            cGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTctgcc                                                          <  2:1037818/140‑1 (MQ=255)                                                                            cGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTctgcc                                                          >  2:503120/1‑140 (MQ=255)                                                                            cGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTctgcc                                                          >  1:912798/1‑140 (MQ=255)                                                                                      cgtcgCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGt                                                >  2:75149/1‑140 (MQ=255)                                                                                      cgtcgCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGt                                                >  1:925304/1‑140 (MQ=255)                                                                                         cgCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGtttt                                             >  2:222237/1‑140 (MQ=255)                                                                                         cgCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGttt                                              <  2:924648/139‑1 (MQ=255)                                                                                         cgCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGttt                                              >  1:924648/1‑139 (MQ=255)                                                                                            ttCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGc                                          <  1:229424/140‑1 (MQ=255)                                                                                                  gTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTtgctg                                    >  2:753932/1‑140 (MQ=255)                                                                                                    aTGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTtgctgct                                  <  1:675789/140‑1 (MQ=255)                                                                                                      gCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGttt                                              <  1:960786/126‑1 (MQ=255)                                                                                                      gCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGttt                                              >  2:960786/1‑126 (MQ=255)                                                                                                      gCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTtgctgctgc                                <  1:886680/140‑1 (MQ=255)                                                                                                                  ttCCTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCa                    <  1:274525/140‑1 (MQ=255)                                                                                                                    ccTCTGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCaaa                  >  1:545815/1‑140 (MQ=255)                                                                                                                     ctctGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAg                 <  1:529524/140‑1 (MQ=255)                                                                                                                      tctGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAgc                <  1:753932/140‑1 (MQ=255)                                                                                                                       ctGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAgcg               <  2:364314/140‑1 (MQ=255)                                                                                                                       ctGTACTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAgcg               <  1:941522/140‑1 (MQ=255)                                                                                                                            tttACCTGGCACGG‑GGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAGCGCAata          <  2:675666/139‑1 (MQ=255)                                                                                                                            cTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAGCGCAata          <  1:49984/140‑1 (MQ=255)                                                                                                                            cTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAGCGCAata          <  1:325294/140‑1 (MQ=255)                                                                                                                            cTTACCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAGCGCAata          <  2:617932/140‑1 (MQ=255)                                                                                                                               aCCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCa                    <  1:543782/127‑1 (MQ=255)                                                                                                                               aCCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCa                    >  2:543782/1‑127 (MQ=255)                                                                                                                               aCCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAgcg               >  2:913102/1‑132 (MQ=255)                                                                                                                               aCCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAgcg               <  1:913102/132‑1 (MQ=255)                                                                                                                               aCCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAGCGCAatatat       >  2:25357/1‑140 (MQ=255)                                                                                                                               aCCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAGCGCAatatat       >  2:1058600/1‑140 (MQ=255)                                                                                                                               aCCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAGCGCAata          <  2:248958/137‑1 (MQ=255)                                                                                                                               aCCTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAGCGCAata          >  1:248958/1‑137 (MQ=255)                                                                                                                                 cTGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAGCGCAATATATcc     <  1:1058600/140‑1 (MQ=255)                                                                                                                                  tGGCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAGCGCAATATATccc    >  2:526449/1‑140 (MQ=255)                                                                                                                                    gCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCa                    >  2:985647/1‑122 (MQ=255)                                                                                                                                    gCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCa                    <  1:985647/122‑1 (MQ=255)                                                                                                                                    gCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAGCGCAATATATc      >  2:898897/1‑136 (MQ=255)                                                                                                                                    gCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAGCGCAATATATc      <  1:898897/136‑1 (MQ=255)                                                                                                                                    gCAC‑AGGGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAGCGCAATATATCCCtt  >  2:467896/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       CATTATTTATGACCAAAGCCGATAAATGCGCGAAAGAGGCGGATACGGCAGCGTTTTCCCGGCTTAACTGGACAACGATGCTCATCGTCGCTTCAGCGTATGCGGTGATTGCTTTCCTCTGTACTTACCTGGCAC‑A‑GGGGGCGATGCAGGCGCTGGTGAAAGCGATGCCCGCCTGGCTGACCCACGGCTTTGAAGTGGCTGGCGGTATTCTGCCTGCCGTTGGTTTTGGCTTGCTGCTGCGCGTAATGTTCAAAGCGCAATATATCCCTT  >  NC_000913/3285040‑3285309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑