Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,982,684	(C)8→9	noncoding (79/87 nt)	leuT →	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,982,676	1	.	C	100.0%	178.2 / NA	50	noncoding (71/87 nt)	leuT	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (29/21);  total (29/21)

GATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAC  >  NC_000913/3982539‑3982809                                                                                                                                           |                                                                                                                                      gATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑cc                                                                                                                                     <  1:6539/140‑1 (MQ=255)   ttGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑cccc                                                                                                                                   <  2:337527/140‑1 (MQ=255)      tgATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑cccc                                                                                                                                   >  2:57404/1‑137 (MQ=255)      tgATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑cccc                                                                                                                                   <  1:57404/137‑1 (MQ=255)           ccAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑ccccccccctcg                                                                                                                           >  1:325683/1‑136 (MQ=255)            cAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑ccccccccctcgc                                                                                                                          >  1:72909/1‑135 (MQ=255)                     tGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACt                                                                                                                  >  1:624720/1‑140 (MQ=255)                                           ccccATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAAtt                                                                                            >  1:569229/1‑140 (MQ=255)                                            cccATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTc                                                                                           >  2:1039146/1‑140 (MQ=255)                                            cccATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTc                                                                                           >  1:491/1‑140 (MQ=255)                                                  attaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAg                                                                                     <  1:716172/140‑1 (MQ=255)                                                  attaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAg                                                                                     <  1:696339/140‑1 (MQ=255)                                                           tgtgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaa                                                                                        >  1:693878/1‑128 (MQ=255)                                                           tgtgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaa                                                                                        <  2:693878/128‑1 (MQ=255)                                                           tgtgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                                                                            >  1:126207/1‑140 (MQ=255)                                                           tgtgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                                                                            >  2:504641/1‑140 (MQ=255)                                                              gACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGc                                                                         <  2:746551/140‑1 (MQ=255)                                                                 aaTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAg                                                                      >  1:607024/1‑140 (MQ=255)                                                                   tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTa                                                                    >  1:641937/1‑140 (MQ=255)                                                                   tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTa                                                                    >  1:437246/1‑140 (MQ=255)                                                                    gCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAg                                                                   <  1:921977/140‑1 (MQ=255)                                                                     cGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgc                                                                  >  1:833545/1‑140 (MQ=255)                                                                              cGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGCGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTgg                                                         >  1:676401/1‑140 (MQ=255)                                                                                 aaTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAg                                                      <  2:624720/140‑1 (MQ=255)                                                                                       tAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAc                                                >  2:758028/1‑140 (MQ=255)                                                                                           cgcgCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGt                                            >  1:652093/1‑140 (MQ=255)                                                                                             cgcTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGttt                                          >  2:338215/1‑140 (MQ=255)                                                                                                tAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTggg                                       >  2:224332/1‑140 (MQ=255)                                                                                                  gCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGAc                                     <  1:127187/140‑1 (MQ=255)                                                                                                     tCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAg                                  <  1:224332/140‑1 (MQ=255)                                                                                                        ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGAc                                     <  1:551919/134‑1 (MQ=255)                                                                                                        ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGAc                                     >  2:551919/1‑134 (MQ=255)                                                                                                        ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTgg                               >  2:874855/1‑140 (MQ=255)                                                                                                            ttAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTgg                                        >  2:95757/1‑127 (MQ=255)                                                                                                            ttAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTgg                                        <  1:95757/127‑1 (MQ=255)                                                                                                            ttAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAg                                  <  1:429618/133‑1 (MQ=255)                                                                                                            ttAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAg                                  >  2:429618/1‑133 (MQ=255)                                                                                                            ttAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCg                           >  1:211957/1‑140 (MQ=255)                                                                                                            ttAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCg                           >  1:320965/1‑140 (MQ=255)                                                                                                            ttAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCg                           >  1:667405/1‑140 (MQ=255)                                                                                                             tAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCgg                          <  1:1039146/140‑1 (MQ=255)                                                                                                                   ccTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTc                    >  1:842159/1‑140 (MQ=255)                                                                                                                   ccTTACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTc                    <  2:569229/140‑1 (MQ=255)                                                                                                                      tACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGaa                 <  2:320965/140‑1 (MQ=255)                                                                                                                      tACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGaa                 <  2:833545/140‑1 (MQ=255)                                                                                                                      tACGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGaa                 <  2:211957/140‑1 (MQ=255)                                                                                                                       aCGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGtt                     >  2:533324/1‑135 (MQ=255)                                                                                                                       aCGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGtt                     <  1:533324/135‑1 (MQ=255)                                                                                                                       aCGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAAt                >  1:794422/1‑140 (MQ=255)                                                                                                                       aCGGACGTGGGGGTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAAt                >  1:277094/1‑140 (MQ=255)                                                                                                                                ggggTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCg       >  2:1018446/1‑140 (MQ=255)                                                                                                                                ggagTTCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCg       >  1:374763/4‑140 (MQ=255)                                                                                                                                     tCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  2:378912/140‑1 (MQ=255)                                                                                                                                     tCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  2:277094/140‑1 (MQ=255)                                                                                                                                     tCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  1:338215/140‑1 (MQ=255)                                                                                                                                     tCAAGTCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  1:874855/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                      GATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAC  >  NC_000913/3982539‑3982809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑