| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,269,672 | (A)7→8 | coding (259/714 nt) | aphA → | acid phosphatase/phosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,269,665 | 1 | . | A | 95.7% | 149.4 / ‑0.3 | 46 | coding (252/714 nt) | aphA | acid phosphatase/phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base A (20/24); total (21/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01 | |||||||||||
CACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCTGATTGATATGCATGTA > NC_000913/4269544‑4269794 | cACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGcc > 1:313231/1‑140 (MQ=255) cccATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAAAACCTTCTCGCCa < 2:1003414/140‑1 (MQ=255) ccATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGa < 2:925895/140‑1 (MQ=255) cattcattGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGaa > 1:112877/1‑140 (MQ=255) ttcattGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAg < 1:612324/140‑1 (MQ=255) ttCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTAt < 1:891961/140‑1 (MQ=255) ttCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTAt > 2:582936/1‑140 (MQ=255) tCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATc > 2:11812/1‑140 (MQ=255) tCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGaa < 1:11812/140‑1 (MQ=255) tCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGaa < 2:677678/140‑1 (MQ=255) gCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGaa < 2:768361/138‑1 (MQ=255) gCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGaa > 1:768361/1‑138 (MQ=255) gCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGaaaa > 1:747896/1‑140 (MQ=255) cacaAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGaaaaa > 2:1017771/1‑140 (MQ=255) cacaAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGaaaaa > 1:311136/1‑140 (MQ=255) tGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCtgtg < 2:311136/140‑1 (MQ=255) ccTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGaa > 1:456525/1‑140 (MQ=255) cTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGaaa > 1:717591/1‑140 (MQ=255) cGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACa < 1:695632/140‑1 (MQ=255) aTGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTggg < 2:112877/140‑1 (MQ=255) ggCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGAt < 1:508431/140‑1 (MQ=255) tGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCt < 1:102130/130‑1 (MQ=255) tGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAAtt < 2:313231/140‑1 (MQ=255) tGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCt > 2:102130/1‑130 (MQ=255) gggttGATATCGATGACACGGTACTTTTTTTCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAg > 2:95588/4‑140 (MQ=255) cGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGGAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCaaaa > 1:206531/1‑140 (MQ=255) acacGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGt < 1:592891/140‑1 (MQ=255) acGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGtcg > 2:609433/1‑140 (MQ=255) tACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGtcgctcg > 1:673258/1‑140 (MQ=255) tACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGtcgctcgc > 1:547053/1‑140 (MQ=255) aCTTTTTTCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGtcgctcgc > 2:449486/1‑140 (MQ=255) ttttttCCAGTCCGGGCTTCTGGCGCGGC‑‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAg < 2:547053/140‑1 (MQ=255) tCCAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCtga < 2:673258/140‑1 (MQ=255) ccAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCtgat < 1:563323/140‑1 (MQ=255) cAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCtgatt < 2:747896/140‑1 (MQ=255) cAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCtgatt < 2:717591/140‑1 (MQ=255) cAGTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCtgatt > 2:535377/1‑140 (MQ=255) gTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCtgattga < 2:456525/140‑1 (MQ=255) gTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCtgattga < 1:609433/140‑1 (MQ=255) gTCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCtgattga < 1:582936/140‑1 (MQ=255) tCCGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCtgattgat > 2:764524/1‑140 (MQ=255) ccGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCTGATTGata < 1:764524/140‑1 (MQ=255) ccGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCTGATTGata < 2:1074855/140‑1 (MQ=255) cGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCtgat > 2:366397/1‑134 (MQ=255) cGGGCTTCTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCtgat < 1:366397/134‑1 (MQ=255) cTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATTTGAAAAATCCTGTGTTCTTGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCTGATTGATATGCATGTa < 2:603413/140‑1 (MQ=255) cTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCTGATTGATATGCATGTa < 2:467970/140‑1 (MQ=255) cTGGCGCGGCA‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCTGATTGATATGCATGTa > 1:651948/1‑140 (MQ=255) | CACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑‑AAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCTGATTGATATGCATGTA > NC_000913/4269544‑4269794 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |