Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,215	(G)8→9	noncoding (72/87 nt)	leuP ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,207	1	.	G	95.6%	146.9 / 0.3	45	noncoding (80/87 nt)	leuP	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base G (19/24);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-01

TAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCC  >  NC_000913/4606069‑4606341                                                                                                                                             |                                                                                                                                           tAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGa                                                                                                                                             <  1:644127/140‑1 (MQ=255)       tAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGACATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑ggggg‑g                                                                                                                                    >  2:306212/1‑140 (MQ=255)          ttGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑ggggg‑gggg                                                                                                                                 >  2:914958/1‑139 (MQ=255)          ttGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑ggggg‑gggg                                                                                                                                 >  2:246035/1‑139 (MQ=255)                 gagggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGaa                                                                                                                          <  1:246035/137‑1 (MQ=255)                 gagggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGaa                                                                                                                          <  1:914958/137‑1 (MQ=255)                     gggggggACTTGAACCCCCACGTCCGTGAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAAcccc                                                                                                                      <  1:457098/140‑1 (MQ=255)                            aCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTcc                                                                                                               >  1:601048/1‑140 (MQ=255)                                     cccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTCAACCCCCACGTCCGTAAGAaca                                                                                                      >  1:458637/1‑140 (MQ=255)                                              gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCt                                                                                            <  2:601048/140‑1 (MQ=255)                                                      acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑cc                                                                                             >  2:63669/1‑131 (MQ=255)                                                      acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑cc                                                                                             <  1:63669/131‑1 (MQ=255)                                                             ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCt                                                                                            <  1:219917/125‑1 (MQ=255)                                                             ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCt                                                                                            >  2:219917/1‑125 (MQ=255)                                                                gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTAcc                                                                          >  1:559967/1‑140 (MQ=255)                                                                 aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCa                                                                         >  2:952514/1‑140 (MQ=255)                                                                 aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCa                                                                         <  1:306212/140‑1 (MQ=255)                                                                  aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCaa                                                                        >  1:844265/1‑140 (MQ=255)                                                                  aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCaa                                                                        >  2:724367/1‑140 (MQ=255)                                                                      aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTcc                                                                    >  1:164118/1‑140 (MQ=255)                                                                      aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTATTCTTA‑‑‑‑ATCTGATATCGCCTCG‑TTGGTGCGA‑‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                              <  1:1022258/140‑1 (MQ=14)                                                                       gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCACGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCg                                                                   <  2:846662/140‑1 (MQ=255)                                                                        cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  >  1:515385/1‑140 (MQ=255)                                                                        cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGAGATCGCCTCGTTTGGGGCGA‑‑GGGGGTGGGCC‑TTGAACCCCCACGCCCGTGACACCACTAACA‑CCTGATGATAGCGCGGCCACCAATTCCGc                                                                  >  2:882764/1‑140 (MQ=25)                                                                            tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                              >  1:454685/1‑140 (MQ=255)                                                                                ccAATTCCGGCACCTTCGCACAGTCATCTTACTTTTTTTGAAATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                          <  1:780001/140‑1 (MQ=255)                                                                                 cAATTCCGCCACCTACGCAAAGTCATCTTAC‑TTTTTTGATATCGCCTCNTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTANCGNGTCTACCAATTCCGCCACCTTCGcc                                                        <  2:503978/140‑1 (MQ=18)                                                                                  aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                        <  2:164118/140‑1 (MQ=255)                                                                                  aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                        <  1:724367/140‑1 (MQ=255)                                                                                  aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                        <  2:844265/140‑1 (MQ=255)                                                                                   aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                                                       <  1:952514/140‑1 (MQ=255)                                                                                    ttCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                                                       <  1:284193/140‑1 (MQ=255)                                                                                    ttCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCa                                                      <  2:559967/140‑1 (MQ=255)                                                                                       cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  <  2:269787/125‑1 (MQ=255)                                                                                       cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  >  1:269787/1‑125 (MQ=255)                                                                                        gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACAGTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc                                                  >  2:970703/1‑140 (MQ=255)                                                                                         ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCCCCACCTTCGCCCCGTGCg                                                 >  1:665526/1‑140 (MQ=255)                                                                                           aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                               <  2:454685/140‑1 (MQ=255)                                                                                            ccTTCGAACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                               <  1:429383/140‑1 (MQ=255)                                                                                                         aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTgg                                 >  2:830328/1‑140 (MQ=255)                                                                                                                   ttttGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGc                       >  1:978396/1‑140 (MQ=255)                                                                                                                    tttGATATCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCg                      >  2:454124/1‑140 (MQ=255)                                                                                                                          atCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCGCGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                <  1:882764/140‑1 (MQ=255)                                                                                                                          atCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                >  2:992665/1‑140 (MQ=255)                                                                                                                          atCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                <  1:992665/140‑1 (MQ=255)                                                                                                                          atCGCCTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                >  1:65858/1‑140 (MQ=255)                                                                                                                              ccTCGTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGa            >  2:57252/1‑140 (MQ=255)                                                                                                                                  gTTTGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTg        <  1:970703/140‑1 (MQ=255)                                                                                                                                   tttGGTGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGa       <  2:515385/140‑1 (MQ=255)                                                                                                                                        tGCGAG‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAcccc  <  1:774730/140‑1 (MQ=255)                                                                                                                                        tGCGA‑‑GGGGG‑GGGGCTTTGAACCCCCCCGTCCGTAAGAA‑ACTAACACCCTGAAGCTAGCGGGCTAACCATTTCGGCAACCTTCCCCCGGGGGGGGCAATTTAAAGGGGGTTTTTGGTGCGAGGGGGGGGCCTTGAAcccc  >  2:535006/1‑140 (MQ=12)                                                                                                                                             |                                                                                                                                           TAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GGGGG‑GGGAC‑TTGAACCCCCACGTCCGTAAGAACACTAACA‑CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCC  >  NC_000913/4606069‑4606341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑