Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,927,241	(C)6→7	coding (1599/2061 nt)	ptrB ←	oligopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,927,235	1	.	C	98.6%	255.3 / ‑3.4	72	coding (1605/2061 nt)	ptrB	oligopeptidase B
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (31/40);  total (32/40)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TACCCCACTCTTCAAACTCACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATG‑CCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCATCAAGATAATCATTAAACGTATTTTTCTTCTTCAGAAATTTTCCGTCTTCGTACC  >  NC_000913/1927096‑1927365                                                                                                                                             |                                                                                                                                    tACCCCACTCTTCAAACTCACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATg                                                                                                                                      <  2:653394/140‑1 (MQ=255)  aCCCCACTCTTCAAACTCACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATG‑c                                                                                                                                    >  2:523707/1‑140 (MQ=255)  aCCCCACTCTTCAAACTCACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATG‑c                                                                                                                                    >  1:690062/1‑140 (MQ=255)    cccACTCTTCAAACTCACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATG‑ccc                                                                                                                                  <  1:589245/140‑1 (MQ=255)    cccACTCTTCAAACTCACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATG‑ccc                                                                                                                                  >  1:275548/1‑140 (MQ=255)         tctTCAAACTCACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATG‑cccccccg                                                                                                                             <  1:929496/140‑3 (MQ=255)         tctTCAAACTCACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATG‑cccccccg                                                                                                                             <  2:484042/140‑3 (MQ=255)           ttCAAACTCACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATG‑cccccccgca                                                                                                                           >  2:249920/1‑136 (MQ=255)           ttCAAACTCACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATG‑cccccccgca                                                                                                                           >  2:353258/1‑136 (MQ=255)             cAAACTCACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACt                                                                                                                         >  2:994805/1‑140 (MQ=255)             cAAACTCACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACt                                                                                                                         >  1:969929/1‑140 (MQ=255)             cAAACTCACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACt                                                                                                                         >  2:1047206/1‑140 (MQ=255)                   cACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCATCAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGcc                                                                                                                   <  2:969929/140‑1 (MQ=255)                   cACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGcc                                                                                                                   <  1:353258/140‑1 (MQ=255)                   cACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGcc                                                                                                                   <  1:1047206/140‑1 (MQ=255)                   cACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGcc                                                                                                                   <  1:619562/140‑1 (MQ=255)                   cACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGcc                                                                                                                   <  2:275548/140‑1 (MQ=255)                   cACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGcc                                                                                                                   <  1:249920/140‑1 (MQ=255)                   cACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGcc                                                                                                                   <  1:530321/140‑1 (MQ=255)                   cACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGcc                                                                                                                   >  2:619562/1‑140 (MQ=255)                   cACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAACAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGcc                                                                                                                   <  1:652274/140‑1 (MQ=255)                     ccAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCa                                                                                                                 <  1:571383/140‑1 (MQ=255)                     ccAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCa                                                                                                                 >  2:571383/1‑140 (MQ=255)                     ccAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCa                                                                                                                 >  2:305495/1‑140 (MQ=255)                      cAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCAt                                                                                                                >  2:990544/1‑140 (MQ=255)                        gTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCg                                                                                                              <  1:487810/140‑1 (MQ=255)                               gagGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACa                                                                                                       <  2:627548/140‑1 (MQ=255)                                     ttGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCg                                                                                                  >  1:526455/1‑139 (MQ=255)                                     ttGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCg                                                                                                  <  2:526455/139‑1 (MQ=255)                                     ttGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGa                                                                                                 >  2:548726/1‑140 (MQ=255)                                     ttGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGa                                                                                                 >  2:511091/1‑140 (MQ=255)                                     ttGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCACATAACAAAGCGa                                                                                                 >  2:374952/1‑140 (MQ=255)                                          tcatcaAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGgag                                                                                            >  1:617022/1‑140 (MQ=255)                                              caAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGcc                                                                                        <  2:236917/140‑1 (MQ=255)                                               aaGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCa                                                                                       <  2:288614/140‑1 (MQ=255)                                               aaGCATCGTTGTT‑CAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCACCGCATAACAAAGCGAAGGAGAGCCAt                                                                                      <  1:964085/140‑1 (MQ=255)                                                  cATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAg                                                                                    >  1:421416/1‑140 (MQ=255)                                                   aTCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGc                                                                                   <  2:496620/140‑1 (MQ=255)                                                   aTCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGc                                                                                   <  2:888139/140‑1 (MQ=255)                                                   aTCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGc                                                                                   <  1:1045943/140‑1 (MQ=255)                                                    tCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGcc                                                                                  <  2:1054736/140‑1 (MQ=255)                                                     cGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGccc                                                                                 <  2:617022/140‑1 (MQ=255)                                                      gttgttACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCa                                                                                >  2:676334/1‑140 (MQ=255)                                                       ttgttACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAg                                                                               >  2:640894/1‑140 (MQ=255)                                                          ttACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGttt                                                                            <  1:548726/140‑1 (MQ=255)                                                               acaTCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACa                                                                       >  1:1030402/1‑140 (MQ=255)                                                               acaTCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACa                                                                       >  2:399965/1‑140 (MQ=255)                                                                caTCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACaa                                                                      >  2:315628/1‑140 (MQ=255)                                                                 aTCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCa                                                                                       <  1:930427/122‑1 (MQ=255)                                                                 aTCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCa                                                                                       >  2:930427/1‑122 (MQ=255)                                                                  tCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGGGAGCCATAGCCCAGTTTTAACAATg                                                                    >  1:1006754/1‑140 (MQ=255)                                                                  tCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGc                                                                                   >  2:877058/1‑125 (MQ=255)                                                                  tCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGc                                                                                   <  1:877058/125‑1 (MQ=255)                                                                        aaCGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAAt                                                                     >  2:913260/1‑133 (MQ=255)                                                                        aaCGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAAt                                                                     <  1:913260/133‑1 (MQ=255)                                                                         aCGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCa                                                             <  2:1030402/140‑1 (MQ=255)                                                                         aCGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCa                                                             <  1:990544/140‑1 (MQ=255)                                                                         aCGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCACATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCa                                                             <  1:374952/140‑1 (MQ=255)                                                                           ggTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAgg                                                           >  1:965958/1‑140 (MQ=255)                                                                            gTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGc                                                          <  1:76955/140‑1 (MQ=255)                                                                             tACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCa                                                         <  2:421416/140‑1 (MQ=255)                                                                             tACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCa                                                         <  2:919332/140‑1 (MQ=255)                                                                             tACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCa                                                         <  1:640894/140‑1 (MQ=255)                                                                             tACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCa                                                         <  1:523707/140‑1 (MQ=255)                                                                             tACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCa                                                         <  2:1030463/140‑1 (MQ=255)                                                                              aCCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCAt                                                        >  2:391391/1‑140 (MQ=255)                                                                                cTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCATCa                                                      >  1:268132/1‑140 (MQ=255)                                                                                   ggCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCa                                                             >  1:1031140/1‑130 (MQ=255)                                                                                   ggCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCa                                                             <  2:1031140/130‑1 (MQ=255)                                                                                    gCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCATCAAGAt                                                  <  1:137942/140‑1 (MQ=255)                                                                                       aTAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCATCAAGATAAt                                               <  1:12007/140‑1 (MQ=255)                                                                                       aTAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCATCAAGATAAt                                               >  2:12007/1‑140 (MQ=255)                                                                                                 ggAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCATCAAGATAATCATTAAACGt                                     >  1:792259/1‑140 (MQ=255)                                                                                                             ggCGTTGATTAATTGCAACGCCCATCAACATG‑CCCCCCTGCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCATCAAGATAATCATTAAACGTATTTttcttctt                         >  2:477124/1‑140 (MQ=255)                                                                                                                 ttGATTAATTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCATCAAGATAATCATTAAACGTATTTTTCTTCTTCAGa                     <  1:391391/140‑1 (MQ=255)                                                                                                                       aaTTGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCATCAAGATAATCATTAAACGTATTTTTCTTCTTCAGAAAtttt               >  2:235318/1‑140 (MQ=255)                                                                                                                          tGCAACGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCATCAAGATAATCATTAAACGTATTTTTCTTCTTCAGAAATTTTCCg            <  2:965958/140‑1 (MQ=255)                                                                                                                             aaCGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCATCAAGATAATCATTAAACGTATTTTTCTTCTTCAGAAATTTTCCGTCt         <  1:994805/140‑1 (MQ=255)                                                                                                                              aCGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCATCAAGATAATCATTAAACGTATTTTTCTTCTTCAGAAATTTTCCGTCtt        <  1:323524/140‑1 (MQ=255)                                                                                                                              aCGCCCATCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCATCAAGATAATCATTAAACGTATTTTTCTTCTTCAGAAATTTTCCGTCtt        >  2:232849/1‑140 (MQ=255)                                                                                                                                    aTCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCATCAAGATAATCATTAAACGTATTTTTCTTCTTCAGAAATTTTCCGTCTTCGTAcc  <  1:305495/140‑1 (MQ=255)                                                                                                                                    aTCAACATGCCCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCATCAAGATAATCATTAAACGTATTTTTCTTCTTCAGAAATTTTCCGTCTTCGTAcc  >  2:465002/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                    TACCCCACTCTTCAAACTCACCAGTGGTAAGAGGAATTGATTCATCAAGCATCGTTGTTACAACATCAACAAACGGTACCTGGGCGATAACGCCGTGGAATAATTCCGGGCGTTGATTAATTGCAACGCCCATCAACATG‑CCCCCC‑GCACTCCCGCCCATCGCATAACAAAGCGAAGGAGAGCCATAGCCCAGTTTTAACAATGCATCGCAGGCATCAAGATAATCATTAAACGTATTTTTCTTCTTCAGAAATTTTCCGTCTTCGTACC  >  NC_000913/1927096‑1927365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑