Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,532,530	T→C	V41A (GTT→GCT)	cysK →	O‑acetylserine sulfhydrylase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,532,530	0	T	C	100.0%	236.3 / NA	65	V41A (GTT→GCT)	cysK	O‑acetylserine sulfhydrylase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/42);  total (23/42)

AGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTA  >  NC_000913/2532394‑2532665                                                                                                                                         |                                                                                                                                        aGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTaa                                                                                                                                      <  2:246270/140‑1 (MQ=255) aGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTaa                                                                                                                                      >  2:294924/1‑140 (MQ=255) aGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTaa                                                                                                                                      <  2:783714/140‑1 (MQ=255) aGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGAT‑ACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAg                                                                                                                                     <  2:287667/140‑1 (MQ=255)             gCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCgg                                                                                                                          >  2:449504/1‑140 (MQ=255)             gCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCgg                                                                                                                          >  2:56263/1‑140 (MQ=255)                aTGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGc                                                                                                                       >  2:815003/1‑140 (MQ=255)                 tGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCACATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGcc                                                                                                                      >  1:998771/1‑140 (MQ=255)                  gAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCa                                                                                                                     <  2:242780/140‑1 (MQ=255)                   aGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCaa                                                                                                                    <  2:134005/140‑1 (MQ=255)                   aGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCaa                                                                                                                    <  2:590395/140‑1 (MQ=255)                    gTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAAc                                                                                                                   <  2:588017/140‑1 (MQ=255)                    gTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAAc                                                                                                                   <  2:130124/140‑1 (MQ=255)                       aGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAAc                                                                                                                   >  1:555738/1‑137 (MQ=255)                       aGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAAc                                                                                                                   <  2:555738/137‑1 (MQ=255)                       aGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATg                                                                                                                >  2:192606/1‑140 (MQ=255)                                        tCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGaaaa                                                                                               <  2:360117/140‑1 (MQ=255)                                             gACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGcgcg                                                                                          <  2:170792/140‑1 (MQ=255)                                                  tCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTg                                                                                     <  1:449504/140‑1 (MQ=255)                                                          acGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAAcc                                                                             <  2:9783/140‑1 (MQ=255)                                                            gccgcTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAg                                                                           >  1:809861/1‑140 (MQ=255)                                                                cTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGt                                                                       >  2:373431/1‑140 (MQ=255)                                                                    ttCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGaa                                                                   <  2:828496/140‑1 (MQ=255)                                                                     tCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAAc                                                                  <  1:192606/140‑1 (MQ=255)                                                                      cGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGCAGGAGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGTGATGCCGAAAAGCGCGGCGTGCTGATACAAGGCGTTGAACt                                                                 >  2:19751/1‑140 (MQ=255)                                                                      cGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACt                                                                 >  2:1083252/1‑140 (MQ=255)                                                                      cGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACt                                                                 >  2:857232/1‑140 (MQ=255)                                                                        ccTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTgg                                                               >  2:998439/1‑140 (MQ=255)                                                                                   tCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGAcc                                                    <  1:1083252/140‑1 (MQ=255)                                                                                          cGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTa                                             <  1:915697/140‑1 (MQ=255)                                                                                              cGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATAc                                         <  1:83044/140‑1 (MQ=255)                                                                                              cGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATAc                                         >  2:522467/1‑140 (MQ=255)                                                                                              cGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATAc                                         <  1:332325/140‑1 (MQ=255)                                                                                                cATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAGCTGGTTGAACCGACCAGCGGTAATACCg                                       >  2:983362/1‑140 (MQ=255)                                                                                                cATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCg                                       >  2:787707/1‑140 (MQ=255)                                                                                                cATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCg                                       >  2:789558/1‑140 (MQ=255)                                                                                                 aTTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCgg                                      >  1:604306/1‑140 (MQ=255)                                                                                                 aTTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCgg                                      >  2:834022/1‑140 (MQ=255)                                                                                                   tCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGa                                    <  1:324419/140‑1 (MQ=255)                                                                                                   tCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGa                                    <  1:522467/140‑1 (MQ=255)                                                                                                   tCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGa                                    <  1:559525/140‑1 (MQ=255)                                                                                                   tCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGa                                    <  1:834022/140‑1 (MQ=255)                                                                                                   tCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGa                                    <  1:959206/140‑1 (MQ=255)                                                                                                        cGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAGCTGGTTGAACCGACCAGCGGTAATACCGGGATTGCa                               <  1:983362/140‑1 (MQ=255)                                                                                                        cGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCa                               <  2:512284/140‑1 (MQ=255)                                                                                                        cGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCa                               <  1:19751/140‑1 (MQ=255)                                                                                                        cGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCa                               <  2:809861/140‑1 (MQ=255)                                                                                                        cGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCa                               <  1:857232/140‑1 (MQ=255)                                                                                                        cGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACCGGTTGAACCGACCAGCGGTAATACCGGGATTGCa                               <  1:56263/140‑1 (MQ=255)                                                                                                          aaGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACt                             <  2:998771/140‑1 (MQ=255)                                                                                                          aaGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACt                             <  1:294924/140‑1 (MQ=255)                                                                                                          aaGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACt                             <  1:853715/140‑1 (MQ=255)                                                                                                           aGGTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTg                            <  1:516860/140‑1 (MQ=255)                                                                                                             gTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGc                          <  2:70578/140‑1 (MQ=255)                                                                                                             gTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGc                          <  1:373431/140‑1 (MQ=255)                                                                                                             gTGGAATCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGc                          <  1:664115/140‑1 (MQ=255)                                                                                                                 aaTCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTg                            >  2:618394/1‑134 (MQ=255)                                                                                                                 aaTCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTg                            <  1:618394/134‑1 (MQ=255)                                                                                                                  aTCTCGTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATg                     <  1:625859/140‑1 (MQ=255)                                                                                                                       gTAACCCCAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCt                >  2:268879/1‑140 (MQ=255)                                                                                                                             ccAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCt          <  1:787707/140‑1 (MQ=255)                                                                                                                             ccAGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCt          <  1:1008256/140‑1 (MQ=255)                                                                                                                               aGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTcg        <  2:604306/140‑1 (MQ=255)                                                                                                                               aGCTTCAGCGCTAAGTGCCGTATCGGTGCCAACATGAATTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCGGCTcg        >  2:959521/1‑140 (MQ=255)                                                                                                                                    cAGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGtt   >  2:108214/1‑140 (MQ=255)                                                                                                                                     aGCGCTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTa  >  2:578875/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        AGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTATCGGTCACACGCCGCTGGTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTA  >  NC_000913/2532394‑2532665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑