Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,564,299	T→C	V107A (GTC→GCC)	yffR →	CPZ‑55 prophage; uncharacterized protein YffR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,564,299	0	T	C	100.0%	132.9 / NA	38	V107A (GTC→GCC)	yffR	CPZ‑55 prophage; uncharacterized protein YffR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/20);  total (18/20)

GGTCGCGACTACGCATGGAAACATGTAAAAGAAGACAGGTTGCCATGTCTGTTTGCCGCCGGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGA  >  NC_000913/2564163‑2564431                                                                                                                                         |                                                                                                                                     ggTCGCGACTACGCATGGAAACATGTAAAAGAAGACAGGTTGCCATGTCTGTTTGCCGCCGGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCag                                                                                                                                   >  2:107332/1‑140 (MQ=255)   tCGCGACTACGCATGGAAACATGTAAAAGAAGACAGGTTGCCATGTCTGTTTGCCGCCGGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCagag                                                                                                                                 >  2:275082/1‑140 (MQ=255)                      cATGTAAAAGAAGACAGGTTGCCATGTCTGTTTGCCGCCGGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGa                                                                                                              >  2:328677/1‑140 (MQ=255)                               gaagaCAGGTTGCCATGTCTGTTTGCCGCCGGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACgg                                                                                                     <  2:304860/140‑1 (MQ=255)                               gaagaCAGGTTGCCATGTCTGTTTGCCGCCGGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACgg                                                                                                     <  2:496902/140‑1 (MQ=255)                                aagaCAGGTTGCCATGTCTGTTTGCCGCCGGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGc                                                                                                    <  1:328677/140‑1 (MQ=255)                                  gaCAGGTTGCCATGTCTGTTTGCCGCCGGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCtt                                                                                                  >  1:481883/1‑140 (MQ=255)                                    cAGGTTGTCATGTCTGTTTGCCGCCGGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTg                                                                                                <  2:534108/140‑1 (MQ=255)                                      ggTTGCCATGTCTGTTTGCCGCCGGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGtt                                                                                              <  1:107332/140‑1 (MQ=255)                                       gTTGCCATGTCTGTTTGCCGCCGGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGttt                                                                                             >  1:77837/1‑140 (MQ=255)                                         tGCCATGTCTGTTTGCCGCCGGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTg                                                                                           <  2:670415/140‑1 (MQ=255)                                                tCTGTTTGCCGCCGGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTggtaggta                                                                                    <  2:59858/140‑1 (MQ=255)                                                tCTGTTTGCCGCCGGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTggtaggta                                                                                    <  1:176165/140‑1 (MQ=255)                                                tCTGTTTGCCGCCGGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTggtaggta                                                                                    <  1:158204/140‑1 (MQ=255)                                                    tttGCCGCCGGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGt                                                                                >  1:1083764/1‑140 (MQ=255)                                                         cgccgGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTg                                                                           >  1:108533/1‑140 (MQ=255)                                                          gccgGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGa                                                                          >  1:424625/1‑140 (MQ=255)                                                          gccgGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGa                                                                          >  1:710336/1‑140 (MQ=255)                                                           ccgGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGaa                                                                         <  1:183869/140‑1 (MQ=255)                                                           ccgGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTAAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGaa                                                                         <  2:708393/140‑1 (MQ=255)                                                                         gATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAattgatt                                                           >  2:151393/1‑140 (MQ=255)                                                                          aTAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAattgattg                                                          >  1:858728/1‑140 (MQ=255)                                                                              cATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGaa                                                      >  2:278071/1‑140 (MQ=255)                                                                                    ggTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGcaa                                                >  1:1041588/1‑140 (MQ=255)                                                                                           tACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTaa                                         <  2:481883/140‑1 (MQ=255)                                                                                               aTTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAaacaa                                     >  2:120007/1‑140 (MQ=255)                                                                                                    aGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAAt                                <  2:858728/140‑1 (MQ=255)                                                                                                      cAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAaac                                       <  2:778493/131‑1 (MQ=255)                                                                                                      cAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAaac                                       >  1:778493/1‑131 (MQ=255)                                                                                                        aTGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATacac                            >  2:333866/1‑140 (MQ=255)                                                                                                         tGAAACTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACAcc                           <  1:422736/140‑1 (MQ=255)                                                                                                           aaaCTGAGTTCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCg                         >  1:991245/1‑140 (MQ=255)                                                                                                                    tCAAACTTAAAGCGGCAGGGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGc                <  2:75542/140‑1 (MQ=255)                                                                                                                    tCAAACTTAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGc                <  2:424625/140‑1 (MQ=255)                                                                                                                         cTTAAAGCAGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGt           <  2:946595/140‑1 (MQ=255)                                                                                                                           tAAAGCGGCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGttt         <  1:82993/140‑1 (MQ=255)                                                                                                                           tAAAGCGGCAGCGGCCAGAGAATGCGCCAACG‑‑GCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGttttt       >  2:618168/1‑140 (MQ=255)                                                                                                                              agcggcagcggcCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGtttttt      <  2:1083764/140‑1 (MQ=255)                                                                                                                                  gCAGCGGCCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGa  <  1:151393/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     GGTCGCGACTACGCATGGAAACATGTAAAAGAAGACAGGTTGCCATGTCTGTTTGCCGCCGGGGTTGTTGCTGATAACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGA  >  NC_000913/2564163‑2564431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑