Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,571,410	G→A	G114G (GGC→GGT)	eutE ←	putative aldehyde dehydrogenase, ethanolamine utilization protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,571,410	0	G	A	100.0%	83.7 / NA	25	G114G (GGC→GGT)	eutE	putative aldehyde dehydrogenase, ethanolamine utilization protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/14);  total (11/14)

CAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTA  >  NC_000913/2571277‑2571531                                                                                                                                      |                                                                                                                          cAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTc                                                                                                                     <  1:812792/140‑1 (MQ=255)           caggTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCg                                                                                                           <  1:572349/137‑1 (MQ=255)             tGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGga                                                                                                         <  1:372881/140‑1 (MQ=255)                tGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGACGCCGGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGgagag                                                                                                      <  2:1004216/140‑1 (MQ=255)                   ccGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCATCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGGAGAGAgg                                                                                                   <  1:8500/140‑1 (MQ=255)                           aTCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGGAGAGAGGCACTCAAc                                                                                           <  1:496722/140‑1 (MQ=255)                                   gATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGgtg                                                                                   <  2:696137/140‑1 (MQ=255)                                        cGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCcg                                                                              <  1:420859/140‑1 (MQ=255)                                                       ttGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACg                                                               >  2:145242/1‑140 (MQ=255)                                                                ggTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCa                                                      <  1:638557/140‑1 (MQ=255)                                                                   tAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAAt                                                   <  2:921578/140‑1 (MQ=255)                                                                          aGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCtt                                            >  2:39171/1‑140 (MQ=255)                                                                           ggCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTc                                           >  1:661242/1‑140 (MQ=255)                                                                           ggCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTc                                           >  1:46394/1‑140 (MQ=255)                                                                               tCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAAcg                                       >  1:284242/1‑140 (MQ=255)                                                                               tCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAAcg                                       <  1:265959/140‑1 (MQ=255)                                                                                cACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAAcgc                                      >  1:644424/1‑140 (MQ=255)                                                                                  ccGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACgcgc                                    <  1:507296/140‑1 (MQ=255)                                                                                                         gCGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGc             >  1:738296/1‑140 (MQ=255)                                                                                                          cggtttCAATTAGGGTCAGGCCGTTGTCACCAGGCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCa            >  1:116268/4‑140 (MQ=255)                                                                                                          cGTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCa            >  1:803656/1‑140 (MQ=255)                                                                                                           gTTTTCAATTAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCaa           >  2:507945/1‑140 (MQ=255)                                                                                                                    tAGGGTCAGGCCGTTGTCACCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTa  <  1:24479/140‑1 (MQ=255)                                                                                                                                 ttGTCACCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTa  <  1:814613/127‑1 (MQ=255)                                                                                                                                 ttGTCACCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTa  >  2:814613/1‑127 (MQ=255)                                                                                                                                      |                                                                                                                          CAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTA  >  NC_000913/2571277‑2571531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑