Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,608,802	T→C	S106P (TCA→CCA)	hyfG →	hydrogenase 4 catalytic subunit HyfG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,608,802	0	T	C	100.0%	198.6 / NA	57	S106P (TCA→CCA)	hyfG	hydrogenase 4 catalytic subunit HyfG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/30);  total (27/30)

CGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGTCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGGATCAGCGTCGCCTGGTGTTGCCCGATGACTGGCCGGAAGATATGCATCCGCT  >  NC_000913/2608668‑2608938                                                                                                                                       |                                                                                                                                         cGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGCCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCgccagtc                                                                                                                                     >  2:674471/1‑140 (MQ=255)    tCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTcaca                                                                                                                                  <  2:124568/140‑1 (MQ=255)              cAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTcc                                                                                                                        >  1:527149/1‑140 (MQ=255)                acgacgAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCt                                                                                                                      >  1:154163/1‑140 (MQ=255)                   acgaGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGcc                                                                                                                   <  2:789229/140‑1 (MQ=255)                   acgaGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGcc                                                                                                                   <  2:872605/140‑1 (MQ=255)                   acgaGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGcc                                                                                                                   <  2:611070/140‑1 (MQ=255)                   acgaGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGcc                                                                                                                   <  1:800374/140‑1 (MQ=255)                              cTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTgggg                                                                                                        <  1:598151/140‑1 (MQ=255)                                       cATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCgaga                                                                                               >  2:222143/1‑140 (MQ=255)                                       cATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCgaga                                                                                               >  1:838216/1‑140 (MQ=255)                                       cATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCgaga                                                                                               >  1:814603/1‑140 (MQ=255)                                         ttACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGaaa                                                                                             >  1:207174/1‑140 (MQ=255)                                               ggTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTcgcg                                                                                       <  1:789039/140‑1 (MQ=255)                                                gTTTATTATGCCCTTTCACTGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGa                                                                                      >  2:769240/1‑140 (MQ=255)                                                gTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGa                                                                                      <  1:674471/140‑1 (MQ=255)                                                 tttATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGat                                                                                     <  2:853522/140‑1 (MQ=255)                                                 tttATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGat                                                                                     <  2:838216/140‑1 (MQ=255)                                                 tttATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGat                                                                                     <  2:955998/140‑1 (MQ=255)                                                  ttattaTGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGata                                                                                    <  1:218073/140‑1 (MQ=255)                                                      tatGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCTGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTa                                                                                >  1:698445/1‑140 (MQ=255)                                                      tatGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTa                                                                                <  1:550269/140‑1 (MQ=255)                                                          cccTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTActgg                                                                            <  2:154163/140‑4 (MQ=255)                                                          cccTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACggg                                                                            <  2:353975/140‑1 (MQ=255)                                                           ccTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGat                                                                                     <  2:545433/130‑1 (MQ=255)                                                           ccTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGat                                                                                     >  1:545433/1‑130 (MQ=255)                                                           ccTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGc                                                                           >  1:739231/1‑140 (MQ=255)                                                           ccTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCATCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTcgcg                                                                                       <  1:631261/128‑1 (MQ=255)                                                           ccTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCATCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTcgcg                                                                                       >  2:631261/1‑128 (MQ=255)                                                             tttCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTg                                                                         >  1:623399/1‑140 (MQ=255)                                                              ttCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGa                                                                        >  2:248789/1‑140 (MQ=255)                                                               tCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTa                                                                                >  2:940914/1‑131 (MQ=255)                                                               tCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTa                                                                                <  1:940914/131‑1 (MQ=255)                                                               tCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGAt                                                                       >  1:800597/1‑140 (MQ=255)                                                                  aTGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTcc                                                                    >  2:395844/1‑140 (MQ=255)                                                                       aGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTg                                                               >  2:568661/1‑140 (MQ=255)                                                                       aGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTg                                                               >  1:842557/1‑140 (MQ=255)                                                                       aGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTg                                                               >  1:161081/1‑140 (MQ=255)                                                                        ggggCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTgg                                                              <  2:623399/140‑1 (MQ=255)                                                                           gCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCt                                                           >  1:534597/1‑140 (MQ=255)                                                                           gCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCt                                                           >  1:787066/1‑140 (MQ=255)                                                                           gCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCt                                                           >  1:813506/1‑140 (MQ=255)                                                                                  aaTGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGGAt                                                    <  2:331401/140‑1 (MQ=255)                                                                                      cTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGGATCAGc                                                <  2:161081/140‑1 (MQ=255)                                                                                       tGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGGATCAGCg                                               <  2:739231/140‑1 (MQ=255)                                                                                       tGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGGATCAGCg                                               <  2:793054/140‑1 (MQ=255)                                                                                          aTTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGGATCAGCGTCg                                            >  2:161411/1‑140 (MQ=255)                                                                                            tgtgGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGGATCAGCGTCGcc                                          <  1:555542/140‑1 (MQ=255)                                                                                              tggtgAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGGATCAGCGTCGCCTg                                        >  2:198864/1‑140 (MQ=255)                                                                                                          tGGTCGATGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGGATCAGCGTCGCCTGGTGTTGCCCGAt                            >  1:295435/1‑140 (MQ=255)                                                                                                                 tGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGGATCAGCGTCGCCTGGTGTTGCCCGATGACTGGc                     <  1:222143/140‑1 (MQ=255)                                                                                                                 tGCCGACAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGGATCAGCGTCGCCTGGTGTTGCCCGATGACTGGc                     <  1:769240/140‑1 (MQ=255)                                                                                                                      aCAGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGGATCAGCGTCGCCTGGTGTTGCCCGATGACTGGCCGGaa                >  1:635819/1‑140 (MQ=255)                                                                                                                        aGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGGATCAGCGTCGCCTGGTGTTGCCCGATGACTGGCCGGAAGa              <  2:527149/140‑1 (MQ=255)                                                                                                                        aGTCGGGAGTTTCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGGATCAGCGTCGCCTGGTGTTGCCCGATGACTGGCCGGAAGa              <  2:295435/140‑1 (MQ=255)                                                                                                                                  ttCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGGATCAGCGTCGCCTGGTGTTGCCCGATGACTGGCCGGAAGATATGCATCCg    <  2:207174/140‑1 (MQ=255)                                                                                                                                   tCCGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGGATCAGCGTCGCCTGGTGTTGCCCGATGACTGGCCGGAAGATATGCATCCGc   >  1:253133/1‑140 (MQ=255)                                                                                                                                    ccGCCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGGATCAGCGTCGCCTGGTGTTGCCCGATGACTGGCCGGAAGATATGCATCCGCt  <  2:787066/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         CGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGTCAGTCACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGGATCAGCGTCGCCTGGTGTTGCCCGATGACTGGCCGGAAGATATGCATCCGCT  >  NC_000913/2608668‑2608938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑