Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,653,842	T→C	intergenic (+503/‑13)	sseA → / → ryfA	3‑mercaptopyruvate sulfurtransferase/small regulatory RNA RyfA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,653,842	0	T	C	100.0%	290.2 / NA	81	intergenic (+503/‑13)	sseA/ryfA	3‑mercaptopyruvate sulfurtransferase/small regulatory RNA RyfA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (51/30);  total (51/30)

TTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGTGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGTCGTGATG  >  NC_000913/2653706‑2653978                                                                                                                                         |                                                                                                                                         ttttGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGta                                                                                                                                       <  1:261490/140‑1 (MQ=255)        gCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATc                                                                                                                                  >  1:1080169/1‑138 (MQ=255)        gCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATc                                                                                                                                  <  2:1080169/138‑1 (MQ=255)                  ttCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCt                                                                                                                      <  2:579901/140‑1 (MQ=255)                            ttCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGtc                                                                                                            >  1:888065/1‑140 (MQ=255)                                       tGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACggg                                                                                                 >  1:904324/1‑140 (MQ=255)                                       tGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACggg                                                                                                 >  2:734675/1‑140 (MQ=255)                                       tGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACggg                                                                                                 >  2:589440/1‑140 (MQ=255)                                       tGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACggg                                                                                                 >  1:347215/1‑140 (MQ=255)                                       tGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACggg                                                                                                 >  2:1087026/1‑140 (MQ=255)                                         tATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGgcg                                                                                               <  2:888065/140‑1 (MQ=255)                                               cGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGgcgc                                                                                              <  2:117899/135‑1 (MQ=255)                                               cGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGgcgc                                                                                              <  2:1020771/135‑1 (MQ=255)                                               cGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGgcgc                                                                                              >  1:117899/1‑135 (MQ=255)                                               cGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGgcgc                                                                                              >  1:1020771/1‑135 (MQ=255)                                                gtagtaATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCtt                                                                                        >  2:543820/1‑140 (MQ=255)                                                 tagtaATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCttt                                                                                       >  1:852020/1‑140 (MQ=255)                                                   gtaATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCt                                                                                             >  2:1007541/1‑132 (MQ=255)                                                   gtaATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCt                                                                                             <  1:1007541/132‑1 (MQ=255)                                                   gtaATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCt                                                                                         <  1:266471/136‑1 (MQ=255)                                                   gtaATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCt                                                                                         >  2:266471/1‑136 (MQ=255)                                                   gtaATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAg                                                                                     >  1:351195/1‑140 (MQ=255)                                                     aaTGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGa                                                                                   <  1:397885/140‑1 (MQ=255)                                                      aTGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTGTCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGaa                                                                                  >  2:971046/1‑140 (MQ=255)                                                      aTGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGaa                                                                                  >  1:159524/1‑140 (MQ=255)                                                      aTGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGaa                                                                                  <  1:642158/140‑1 (MQ=255)                                                      aTGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGaa                                                                                  >  1:809598/1‑140 (MQ=255)                                                      aTGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGaa                                                                                  <  2:852020/140‑1 (MQ=255)                                                      aTGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGaa                                                                                  >  2:785118/1‑140 (MQ=255)                                                       tGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGaaa                                                                                 >  1:971802/1‑140 (MQ=255)                                                         cAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAgg                                                                               >  1:653828/1‑140 (MQ=255)                                                           aGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGAt                                                                             >  1:58704/1‑140 (MQ=255)                                                           aGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGAt                                                                             >  1:1000908/1‑140 (MQ=255)                                                            gcgcGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGaa                                                                                  <  2:28118/134‑1 (MQ=255)                                                            gcgcGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGaa                                                                                  >  1:28118/1‑134 (MQ=255)                                                            gcgcGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATg                                                                            >  1:861308/1‑140 (MQ=255)                                                             cgcgTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGt                                                                           >  2:423897/1‑140 (MQ=255)                                                             cgcgTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGACTTTAGGAAAGGATGt                                                                           >  1:850708/1‑140 (MQ=255)                                                              gcgTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGtt                                                                          >  2:1049215/1‑140 (MQ=255)                                                                 tctcAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCg                                                                       <  2:809598/140‑1 (MQ=255)                                                                 tctcAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCg                                                                       >  2:646572/1‑140 (MQ=255)                                                                 tctcAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCg                                                                       <  2:159524/140‑1 (MQ=255)                                                                 tctcAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCg                                                                       <  2:904324/140‑1 (MQ=255)                                                                 tctcAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCg                                                                       >  1:564955/1‑140 (MQ=255)                                                                  ctcAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGa                                                                              >  2:846343/1‑132 (MQ=255)                                                                  ctcAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGa                                                                              <  1:846343/132‑1 (MQ=255)                                                                   tcAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTg                                                                     <  2:759487/140‑1 (MQ=255)                                                                     aGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGc                                                                   >  2:838481/1‑140 (MQ=255)                                                                            aCAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAAt                                                            >  2:4429/1‑140 (MQ=255)                                                                            aCAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAAt                                                            >  2:637769/1‑140 (MQ=255)                                                                                tACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAg                                                        <  1:838481/140‑1 (MQ=255)                                                                                tACTCGCCAGTAACTCCCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAg                                                        <  2:971802/140‑1 (MQ=255)                                                                                    cGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGgtgt                                                    >  2:193188/1‑140 (MQ=255)                                                                                        aGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTca                                                <  2:268660/140‑1 (MQ=255)                                                                                        aGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTca                                                >  1:268660/1‑140 (MQ=255)                                                                                          tAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGgtgt                                                    >  1:1081545/1‑134 (MQ=255)                                                                                          tAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGgtgt                                                    <  2:1081545/134‑1 (MQ=255)                                                                                          tAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTcaca                                              >  1:93221/1‑140 (MQ=255)                                                                                          tAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTcaca                                              >  1:394409/1‑140 (MQ=255)                                                                                             ctctctTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAgcg                                           >  2:652077/1‑140 (MQ=255)                                                                                                 ctTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTg                                       >  1:515041/1‑140 (MQ=255)                                                                                                 ctTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTg                                       >  2:55999/1‑140 (MQ=255)                                                                                                         cAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATcgcg                               <  1:1087026/140‑1 (MQ=255)                                                                                                         cAAGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATcgcg                               <  2:93221/140‑1 (MQ=255)                                                                                                          aaGCAAAAGAGAGTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCgg                              >  1:554333/1‑140 (MQ=255)                                                                                                                      gTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTAt                                   <  1:638015/123‑1 (MQ=255)                                                                                                                      gTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTAt                                   >  2:638015/1‑123 (MQ=255)                                                                                                                      gTTATTATTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAg                  >  1:187512/1‑140 (MQ=255)                                                                                                                       ttatcaTTGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAgt                 >  2:799308/1‑140 (MQ=255)                                                                                                                          ttattGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAgtggt              >  2:401900/1‑140 (MQ=255)                                                                                                                          ttattGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAgtggt              >  2:792953/1‑140 (MQ=255)                                                                                                                           tattGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAgtggtg             <  1:971046/140‑1 (MQ=255)                                                                                                                           tattGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAgtggtg             <  1:423897/140‑1 (MQ=255)                                                                                                                           tattGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAgtggtg             <  1:785118/140‑1 (MQ=255)                                                                                                                           tattGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAgtggtg             <  1:734675/140‑1 (MQ=255)                                                                                                                           tattGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAgtggtg             <  2:554333/140‑1 (MQ=255)                                                                                                                            attGTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGc            >  1:329642/1‑140 (MQ=255)                                                                                                                               gTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGt         >  2:707768/1‑140 (MQ=255)                                                                                                                               gTTCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGt         >  1:867892/1‑140 (MQ=255)                                                                                                                                 tCTGTTAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGTCg       >  1:994408/1‑140 (MQ=255)                                                                                                                                      tAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGTTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGTTTGCTATCGCGGCAATATCGCCAGTGGTGCTGTCGTGATg  <  2:1057169/140‑1 (MQ=255)                                                                                                                                      tAGCGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGTCGTGATg  <  2:653828/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGTGTATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGTCGTGATG  >  NC_000913/2653706‑2653978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑