Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,669,673	T→C	A214A (GCT→GCC)	hcaE →	putative 3‑phenylpropionate/cinnamate dioxygenase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,669,673	0	T	C	100.0%	250.7 / NA	69	A214A (GCT→GCC)	hcaE	putative 3‑phenylpropionate/cinnamate dioxygenase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/36);  total (33/36)

ACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCTCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCGCCCGCCCGGTGTGGGAAACCGCCAAAGATGCGCTGCAATTTGGTCAGG  >  NC_000913/2669537‑2669803                                                                                                                                         |                                                                                                                                   aCATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTg                                                                                                                                 <  1:544179/140‑1 (MQ=255)   aTTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGtt                                                                                                                               <  1:970247/140‑1 (MQ=255)   aTTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGtt                                                                                                                               <  1:359432/140‑1 (MQ=255)       ccTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGc                                                                                                                           <  1:977419/140‑1 (MQ=255)        cTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGcc                                                                                                                          >  2:886455/1‑140 (MQ=255)            tATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGc                                                                                                                      >  1:605756/1‑140 (MQ=255)            tATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGc                                                                                                                      >  2:243126/1‑140 (MQ=255)               cTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACCGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTc                                                                                                                   >  1:32536/1‑140 (MQ=255)                 ggATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTg                                                                                                                 <  2:631645/140‑1 (MQ=255)                 ggATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTg                                                                                                                 >  1:789908/1‑140 (MQ=255)                 ggATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTg                                                                                                                 <  1:886455/140‑1 (MQ=255)                 ggATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTg                                                                                                                 <  1:243126/140‑1 (MQ=255)                    tGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCg                                                                                                              <  2:556263/140‑1 (MQ=255)                      gCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGtt                                                                                                            <  1:981022/140‑1 (MQ=255)                      gCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGtt                                                                                                            >  1:376453/1‑140 (MQ=255)                      gCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGtt                                                                                                            <  2:880956/140‑1 (MQ=255)                      gCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGtt                                                                                                            >  1:469903/1‑140 (MQ=255)                      gCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGtt                                                                                                            <  2:143227/140‑1 (MQ=255)                          gCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAgg                                                                                                        >  2:715063/1‑140 (MQ=255)                            tGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGtt                                                                                                            <  1:301131/134‑1 (MQ=255)                            tGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGtt                                                                                                            >  2:301131/1‑134 (MQ=255)                            tGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGt                                                                                                             <  2:409822/133‑1 (MQ=255)                            tGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGt                                                                                                             >  1:409822/1‑133 (MQ=255)                                tcgtcgCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAg                                                                                                  <  1:948094/140‑1 (MQ=255)                                      cgAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGtt                                                                                                            >  2:758857/1‑124 (MQ=255)                                      cgAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGtt                                                                                                            <  1:758857/124‑1 (MQ=255)                                      cgAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGa                                                                                            <  2:572730/140‑1 (MQ=255)                                      cgAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGa                                                                                            <  2:879688/140‑1 (MQ=255)                                      cgAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGa                                                                                            <  2:1064678/140‑1 (MQ=255)                                      caaaGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGa                                                                                            <  2:376453/138‑1 (MQ=255)                                             ggcACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAgatga                                                                                     >  1:697745/1‑140 (MQ=255)                                             ggcACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAgatga                                                                                     >  1:83655/1‑140 (MQ=255)                                              gcACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAgatgat                                                                                    >  1:253767/1‑140 (MQ=255)                                                   gAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAg                                                                               <  1:908817/140‑1 (MQ=255)                                                    aaaTTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGc                                                                              >  1:145501/1‑140 (MQ=255)                                                       ttGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGAt                                                                           <  2:834518/140‑1 (MQ=255)                                                                   tACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGt                                                               <  2:9760/140‑1 (MQ=255)                                                                       aaaGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATg                                                           <  2:145501/140‑1 (MQ=255)                                                                             ggTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACaaa                                                     >  2:48712/1‑140 (MQ=255)                                                                                gATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAAccg                                                  >  2:306414/1‑140 (MQ=255)                                                                                  tCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAAccgcc                                                >  2:674261/1‑140 (MQ=255)                                                                                  tCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAAccgcc                                                >  2:270329/1‑140 (MQ=255)                                                                                     aCTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAAccgcccgc                                             >  1:727123/1‑140 (MQ=255)                                                                                      ctgtcgcTGAAAATTCCCGGAAGGCGAGCTCGACAGGCACAAGTAGCAAACCCCCTTCAGCCATGCTTCTGCCGTTCAGGTATTACGGGCGAGAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACcgcccgcc                                            <  2:697574/134‑1 (MQ=255)                                                                                      cTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACcgcccgcc                                            <  2:605756/140‑1 (MQ=255)                                                                                      cTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACcgcccgcc                                            <  2:253767/140‑1 (MQ=255)                                                                                      cTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACcgcccgcc                                            <  2:469903/140‑1 (MQ=255)                                                                                      cTGTAACTGGAAATTCCCGGCAGAGCAATTCGCCAGTGACCAGTATCATGTCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACcgcccgcc                                            <  2:299290/140‑1 (MQ=255)                                                                                       tGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCgcccgccc                                           >  1:372606/1‑140 (MQ=255)                                                                                           aCTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACcgcccgcc                                            <  2:276879/135‑1 (MQ=255)                                                                                           aCTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACcgcccgcc                                            >  1:276879/1‑135 (MQ=255)                                                                                            cTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCGCCCGCCCGgtgt                                      >  1:353904/1‑140 (MQ=255)                                                                                              ggAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCgcccgccc                                           >  2:1051661/1‑133 (MQ=255)                                                                                              ggAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCgcccgccc                                           <  1:1051661/133‑1 (MQ=255)                                                                                              ggAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCGCCCGCCCGGTGTgg                                    >  1:21906/1‑140 (MQ=255)                                                                                                aaaTTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACcgcccgcc                                            >  2:431153/1‑130 (MQ=255)                                                                                                aaaTTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACcgcccgcc                                            <  1:431153/130‑1 (MQ=255)                                                                                                     cccGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCGCCCGCCCGGTGTGGGAAACCg                             <  2:697745/140‑1 (MQ=255)                                                                                                     cccGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCGCCCGCCCGGTGTGGGAAACCg                             <  2:83655/140‑1 (MQ=255)                                                                                                       cGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCGCCCGCCCGGTGTGGGAAACCGcc                           >  2:579640/1‑140 (MQ=255)                                                                                                         gCAGAGCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCGCCCGCCCGGTGTGGGAAACCGCCaa                         <  2:32536/140‑1 (MQ=255)                                                                                                             agCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCGCCCGCCCGGTGTGGGAAACCGCCAAAGAt                     >  2:1009879/1‑140 (MQ=255)                                                                                                             agCAGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCGCCCGCCCGGTGTGGGAAACCGCCAAAGAt                     >  1:918281/1‑140 (MQ=255)                                                                                                                aGTTCGCCAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCGCCCGCCCGGTGTGGGAAACCGCCAAAGATgcg                  <  1:674261/140‑1 (MQ=255)                                                                                                                       cAGTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCGCCCGCCCGGTGTGGGAAACCGCCAAAGATGCGCTGCAAt           >  1:10604/1‑140 (MQ=255)                                                                                                                         gTGACCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCGCCCGCCCGGTGTGCGAAACCGCCAAAGATGCGCTGCAAttt         >  2:472622/1‑140 (MQ=255)                                                                                                                            aCCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCGCCCGCCCGGTGTGGGAAACCGCCAAAGATgcg                  <  1:361149/128‑1 (MQ=255)                                                                                                                            aCCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCGCCCGCCCGGTGTGGGAAACCGCCAAAGATgcg                  >  2:361149/1‑128 (MQ=255)                                                                                                                            aCCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCGCCCGCCCGGTGTGGGAAACCGCCAAAGATGCGCTGCAATTTGGt      >  2:148990/1‑140 (MQ=255)                                                                                                                            aCCAGTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCGCCCGCCCGGTGTGGGAAACCGCCAAAGATGCGCTGCAATTTGGt      <  1:579640/140‑1 (MQ=255)                                                                                                                                gTATCATGCCCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCGCCCGCCCGGTGTGGGAAACCGCCAAAGATGCGCTGCAATTTGGTCAgg  <  1:1009879/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   ACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATGCTCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAAACCGCCCGCCCGGTGTGGGAAACCGCCAAAGATGCGCTGCAATTTGGTCAGG  >  NC_000913/2669537‑2669803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑