Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,125,139	T→C	K107K (AAA→AAG)	glcF ←	glycolate dehydrogenase, putative iron‑sulfur subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,125,139	0	T	C	100.0%	211.8 / NA	59	K107K (AAA→AAG)	glcF	glycolate dehydrogenase, putative iron‑sulfur subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/25);  total (34/25)

AGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGTTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCG  >  NC_000913/3125008‑3125274                                                                                                                                    |                                                                                                                                        agTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACttt                                                                                                                                 >  2:714254/1‑140 (MQ=255)     ttGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAATATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGc                                                                                                                             <  1:429150/140‑1 (MQ=255)         tctGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGa                                                                                                                         >  1:20792/1‑140 (MQ=255)             aCCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTc                                                                                                                           >  2:140254/1‑134 (MQ=255)             aCCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTc                                                                                                                           <  1:140254/134‑1 (MQ=255)                  ttCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCAc                                                                                                                >  2:556950/1‑140 (MQ=255)                  ttCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCAc                                                                                                                >  1:906769/1‑140 (MQ=255)                       gTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCg                                                                                                           >  1:1012035/1‑140 (MQ=255)                       gTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCg                                                                                                           >  1:474682/1‑140 (MQ=255)                       gTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCg                                                                                                           >  1:1031047/1‑140 (MQ=255)                            aaCGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCGGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCg                                                                                                           >  2:877867/1‑135 (MQ=255)                            aaCGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCGGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCg                                                                                                           <  1:877867/135‑1 (MQ=255)                            aaCGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCg                                                                                                           >  2:873290/1‑135 (MQ=255)                            aaCGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCg                                                                                                           <  1:873290/135‑1 (MQ=255)                            aaCGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATc                                                                                                      >  2:356844/1‑140 (MQ=255)                               ggTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAg                                                                                                   <  2:496979/140‑1 (MQ=255)                                 tCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCa                                                                                                 <  2:474682/140‑1 (MQ=255)                                 tCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCa                                                                                                 >  1:614813/1‑140 (MQ=255)                                    cagcaCCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAAt                                                                                              <  2:906769/140‑1 (MQ=255)                                              ccTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAgcg                                                                                    <  1:412315/140‑1 (MQ=255)                                                tACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGca                                                                                  <  2:230872/140‑1 (MQ=255)                                                tACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGca                                                                                  <  1:356844/140‑1 (MQ=255)                                                 aCCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAgcg                                                                                    <  1:920796/137‑1 (MQ=255)                                                 aCCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAgcg                                                                                    >  2:920796/1‑137 (MQ=255)                                                 aCCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGcac                                                                                 <  2:1031047/140‑1 (MQ=255)                                                 aCCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGcac                                                                                 >  1:1003819/1‑140 (MQ=255)                                                      cGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAg                                                                            >  1:354883/1‑140 (MQ=255)                                                       gTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGa                                                                           >  1:677170/1‑140 (MQ=255)                                                       gTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGa                                                                           >  2:481621/1‑140 (MQ=255)                                                       gTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCAAACCAGa                                                                           >  2:727275/1‑140 (MQ=255)                                                        tCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAgcg                                                                                    >  1:908443/1‑130 (MQ=255)                                                        tCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAgcg                                                                                    <  2:908443/130‑1 (MQ=255)                                                        tCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGaa                                                                          <  2:690571/140‑1 (MQ=255)                                                          aGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAgg                                                                        >  2:541016/1‑140 (MQ=255)                                                            cgcACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGAc                                                                      >  2:832124/1‑140 (MQ=255)                                                            cgcACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGAAc                                                                      >  2:1013080/1‑140 (MQ=255)                                                               ccGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGGCAgg                                                                   <  2:961646/139‑1 (MQ=255)                                                                cGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAggt                                                                  >  1:781260/1‑140 (MQ=255)                                                                         gccgGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTcaca                                                         >  1:158792/1‑140 (MQ=255)                                                                           cgGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAAt                                                       >  1:233094/1‑140 (MQ=255)                                                                             gACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTa                                                     >  1:146147/1‑140 (MQ=255)                                                                                 cgGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTACGGc                                                 <  2:1003819/140‑1 (MQ=255)                                                                                 cgGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTACGGc                                                 <  1:832124/140‑1 (MQ=255)                                                                                 cgGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTACGGc                                                 <  2:614813/140‑1 (MQ=255)                                                                                 cgGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGAACAGGTGGTTTCACAATTACGGc                                                 <  1:1013080/140‑1 (MQ=255)                                                                                           tGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTACGGCAAGTGAGGCa                                       <  2:1012035/140‑1 (MQ=255)                                                                                                cAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACTAGAAGGACAGGTGGTTTCACAATTACGGCAAGTGAGGCAGCGAt                                  <  2:20792/140‑1 (MQ=255)                                                                                                cAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTACGGCAAGTGAGGCAGCGAt                                  <  2:506736/140‑1 (MQ=255)                                                                                                cAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTACGGCAAGTGAGGCAGCGAt                                  <  1:724603/140‑1 (MQ=255)                                                                                                   tCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTACGGCAAGTGAGGCAGCGATCga                               >  1:1084166/1‑140 (MQ=255)                                                                                                        cgcgCAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGc                          >  2:692093/1‑140 (MQ=255)                                                                                                           gcAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTcc                       <  2:677170/140‑1 (MQ=255)                                                                                                           gcAGTATTCGCTCCGGCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTcc                       <  1:714254/140‑1 (MQ=255)                                                                                                                         ggCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGt         >  1:380023/1‑140 (MQ=255)                                                                                                                         ggCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGt         >  1:252336/1‑140 (MQ=255)                                                                                                                         ggCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGt         >  1:892053/1‑140 (MQ=255)                                                                                                                         ggCAGTGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGt         >  2:462745/1‑140 (MQ=255)                                                                                                                              tGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCtg                     >  1:665556/1‑123 (MQ=255)                                                                                                                              tGGGCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCtg                     <  2:665556/123‑1 (MQ=255)                                                                                                                                ggCGCTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCg  >  2:621493/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        AGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAAGACCGCCGGACGCGGCACTACCTGGCGCAATCCTTCGCGCAGTATTCGCTCCGGCAGTGGGCGTTTCACTTTCTGCTCGACAATATCACGCCCGATATCCAGCAAATTGTGATAGCGCACACCAGAAGGACAGGTGGTTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCG  >  NC_000913/3125008‑3125274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑