Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,128,950	A→C	T227P (ACC→CCC)	glcC →	DNA‑binding transcriptional dual regulator GlcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,128,950	0	A	C	100.0%	243.5 / NA	67	T227P (ACC→CCC)	glcC	DNA‑binding transcriptional dual regulator GlcC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (34/33);  total (34/33)

TGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCG  >  NC_000913/3128813‑3129086                                                                                                                                          |                                                                                                                                         tgtTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGccc                                                                                                                                        >  1:381062/1‑140 (MQ=255)  gtTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCg                                                                                                                                       <  2:1085893/140‑1 (MQ=255)      gCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCACAGCACGCGATCATGTGCGGCCCGTg                                                                                                                                     <  1:794549/138‑1 (MQ=255)      gCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCACAGCACGCGATCATGTGCGGCCCGTg                                                                                                                                     >  2:794549/1‑138 (MQ=255)           cGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGa                                                                                                                              >  1:1008984/1‑140 (MQ=255)           cGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGa                                                                                                                              <  1:1080312/140‑1 (MQ=255)           cGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGa                                                                                                                              >  2:409651/1‑140 (MQ=255)             tAAATACTCTCTACCATCGACTACAGCAAATAAAGCTGATCGAGCGGCAGCATGCGCGGAGCTACAACGAGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAAt                                                                                                                            >  2:914149/1‑140 (MQ=255)                    tctcTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACg                                                                                                                     >  1:19830/1‑140 (MQ=255)                       ctACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGata                                                                                                                  >  1:414186/1‑140 (MQ=255)                        tACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGatat                                                                                                                 <  2:19830/140‑1 (MQ=255)                        tACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGatat                                                                                                                 >  1:966078/1‑140 (MQ=255)                        tACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGatat                                                                                                                 <  2:1001019/140‑1 (MQ=255)                        tACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGatat                                                                                                                 >  2:941495/1‑140 (MQ=255)                        tACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCACCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGatat                                                                                                                 <  2:966078/140‑1 (MQ=255)                         aCCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCa                                                                                                                       >  2:732018/1‑133 (MQ=255)                         aCCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCa                                                                                                                       <  1:732018/133‑1 (MQ=255)                          ccATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCg                                                                                                               <  2:168978/140‑1 (MQ=255)                            aTCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAg                                                                                                             >  1:835073/1‑140 (MQ=255)                             tCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGc                                                                                                            >  1:1027886/1‑140 (MQ=255)                                aCCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTgg                                                                                                         >  2:761589/1‑140 (MQ=255)                                 ccACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGa                                                                                                        <  1:254413/140‑1 (MQ=255)                                 ccACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGa                                                                                                        <  2:381062/140‑1 (MQ=255)                                  cacaGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGaa                                                                                                       >  2:431733/1‑140 (MQ=255)                                                cAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGAt                                                                                         <  2:835073/140‑1 (MQ=255)                                                   atcgatcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCg                                                                                      >  2:123970/1‑140 (MQ=255)                                                    tcgatcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGc                                                                                     >  2:393526/1‑140 (MQ=255)                                                             aGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGAGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTg                                                                            >  2:948836/1‑140 (MQ=255)                                                               cATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTg                                                                            >  2:742404/1‑138 (MQ=255)                                                               cATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTg                                                                            <  1:742404/138‑1 (MQ=255)                                                                 tGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGc                                                                        <  1:123970/140‑1 (MQ=255)                                                                 tGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGc                                                                        >  1:309115/1‑140 (MQ=255)                                                                       gATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGgaga                                                                  <  1:409651/140‑1 (MQ=255)                                                                        aTCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGAt                                                                 <  1:941495/140‑1 (MQ=255)                                                                           tACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGaa                                                              <  1:208701/140‑1 (MQ=255)                                                                            aCAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAAc                                                             <  1:783657/140‑1 (MQ=255)                                                                            aCAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAAc                                                             >  1:435787/1‑140 (MQ=255)                                                                              aaCGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCt                                                           <  2:1008984/140‑1 (MQ=255)                                                                                cgcgGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAGGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGa                                                         >  2:1022741/1‑140 (MQ=255)                                                                                  cgGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGaa                                                              >  1:202429/1‑133 (MQ=255)                                                                                  cgGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGaa                                                              <  2:202429/133‑1 (MQ=255)                                                                                  cgGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGt                                                       >  2:553923/1‑140 (MQ=255)                                                                                  cgGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGt                                                       >  2:605164/1‑140 (MQ=255)                                                                                  cgGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGt                                                       >  2:172555/1‑140 (MQ=255)                                                                                     tgtTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAg                                                    <  1:431733/140‑1 (MQ=255)                                                                                      gtTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGc                                                   <  1:517741/140‑1 (MQ=255)                                                                                          cAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGt                                               <  1:172555/140‑1 (MQ=255)                                                                                             cGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTAtt                                            <  2:55575/140‑1 (MQ=255)                                                                                               gCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTaa                                          >  1:609231/1‑140 (MQ=255)                                                                                               gCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTaa                                          >  1:982638/1‑140 (MQ=255)                                                                                                 tGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCT‑GTATTAAATc                                       >  2:86629/1‑140 (MQ=255)                                                                                                  gCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATc                                       >  1:466543/1‑140 (MQ=255)                                                                                                   ccGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCt                                      <  1:914149/140‑1 (MQ=255)                                                                                                   ccGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCt                                      <  2:1010743/140‑1 (MQ=255)                                                                                                      cACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCt                                   <  2:847141/140‑1 (MQ=255)                                                                                                      cACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCt                                   <  1:733559/140‑1 (MQ=255)                                                                                                      cACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCt                                   <  2:251773/140‑1 (MQ=255)                                                                                                           cGCCCAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCT‑GTATTAAATCTGCTTTTCAt                             <  1:86629/140‑1 (MQ=255)                                                                                                             cccAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATa                            <  1:328265/140‑1 (MQ=255)                                                                                                              ccAGCGCGCAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATAc                           <  1:605164/140‑1 (MQ=255)                                                                                                                  cgcgcAGCACGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATc                       >  1:735114/1‑140 (MQ=255)                                                                                                                     gcagcaCGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGt                    <  2:341832/140‑1 (MQ=255)                                                                                                                     gcagcaCGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGt                    <  1:553923/140‑1 (MQ=255)                                                                                                                       agcaCGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTaa                  >  2:245872/1‑140 (MQ=255)                                                                                                                       agcaCGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTaa                  >  1:927122/1‑140 (MQ=255)                                                                                                                       agcaCGCGATCATGTGCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTaa                  >  2:974168/1‑140 (MQ=255)                                                                                                                                   tgtgCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGc      >  2:242059/1‑140 (MQ=255)                                                                                                                                   tgtgCGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGc      >  1:463258/1‑140 (MQ=255)                                                                                                                                       cGGCCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGTTGCCGCTGGAGATGAAGCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCg  >  1:468638/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         TGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCG  >  NC_000913/3128813‑3129086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑