Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,173,233	T→C	V31A (GTA→GCA)	ygiN →	putative quinol monooxygenase YgiN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,173,233	0	T	C	100.0%	256.0 / NA	71	V31A (GTA→GCA)	ygiN	putative quinol monooxygenase YgiN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/32);  total (39/32)

AAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCA  >  NC_000913/3173098‑3173365                                                                                                                                        |                                                                                                                                     aaaTTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTg                                                                                                                                  >  2:848986/1‑140 (MQ=255) aaaTTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTg                                                                                                                                  <  2:779484/140‑1 (MQ=255)  aaTTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGa                                                                                                                                 <  1:827118/140‑1 (MQ=255)        tGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAaagaag                                                                                                                           <  1:45047/140‑1 (MQ=255)          gTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAaagaagaa                                                                                                                         >  1:621138/1‑140 (MQ=255)                        aGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTa                                                                                                           <  2:1016084/140‑1 (MQ=255)                        aGGCCTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTa                                                                                                           >  2:820690/1‑140 (MQ=255)                         ggCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTAt                                                                                                          >  1:470147/1‑140 (MQ=255)                             ttAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATgcgc                                                                                                      <  1:944075/140‑1 (MQ=255)                             ttAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATgcgc                                                                                                      <  1:107460/140‑1 (MQ=255)                             ttAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATgcgc                                                                                                      <  2:232192/140‑1 (MQ=255)                                    gAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAAtggt                                                                                               >  2:409405/1‑140 (MQ=255)                                    gAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAAtggt                                                                                               >  1:371099/1‑140 (MQ=255)                                     aGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAAtggtg                                                                                              >  2:34362/1‑140 (MQ=255)                                        tAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGAt                                                                                           >  1:774804/1‑140 (MQ=255)                                         aaCCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGAtt                                                                                          >  1:229530/1‑140 (MQ=255)                                         aaCCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGAtt                                                                                          >  2:805680/1‑140 (MQ=255)                                               gCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATgcgc                                                                                                      <  2:208556/122‑1 (MQ=255)                                               gCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATgcgc                                                                                                      >  1:208556/1‑122 (MQ=255)                                               gCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAg                                                                                    >  2:486827/1‑140 (MQ=255)                                               gCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAg                                                                                    <  1:473766/140‑1 (MQ=255)                                                   aCCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTgg                                                                                >  1:362764/1‑140 (MQ=255)                                                     cGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCg                                                                              >  2:319747/1‑140 (MQ=255)                                                        aaTCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGa                                                                           <  1:107526/140‑1 (MQ=255)                                                             cAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTc                                                                      <  1:319747/140‑1 (MQ=255)                                                             cAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTc                                                                      <  1:486827/140‑1 (MQ=255)                                                             cAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTc                                                                      <  2:621138/140‑1 (MQ=255)                                                               gAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCa                                                                    <  1:34362/140‑1 (MQ=255)                                                               gAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCa                                                                    <  1:376681/140‑1 (MQ=255)                                                                     cGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCg                                                                              >  1:1004883/1‑124 (MQ=255)                                                                     cGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCg                                                                              <  2:1004883/124‑1 (MQ=255)                                                                           cGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCAcc                                                        <  1:521541/140‑1 (MQ=255)                                                                            gTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTc                                                                      <  2:328287/125‑1 (MQ=255)                                                                            gTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTc                                                                      >  1:328287/1‑125 (MQ=255)                                                                            gTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCg                                                       >  1:1009392/1‑140 (MQ=255)                                                                                  gTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCt                                                 >  2:614466/1‑140 (MQ=255)                                                                                      aCATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCa                                                          >  1:533837/1‑127 (MQ=255)                                                                                      aCATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCa                                                          <  2:533837/127‑1 (MQ=255)                                                                                      aCATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCg                                             >  1:911640/1‑140 (MQ=255)                                                                                       cATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGt                                            <  2:898063/140‑1 (MQ=255)                                                                                                 aGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAg                                  >  2:171212/1‑140 (MQ=255)                                                                                                 aGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAg                                  >  1:204751/1‑140 (MQ=255)                                                                                                 aGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAg                                  >  1:279611/1‑140 (MQ=255)                                                                                                 aGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAg                                  >  1:321240/1‑140 (MQ=255)                                                                                                  ggCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGTCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGc                                 <  1:805680/140‑1 (MQ=255)                                                                                                  ggCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGc                                 <  1:614466/140‑1 (MQ=255)                                                                                                                 gTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGc                                 >  2:940983/1‑125 (MQ=255)                                                                                                                 gTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGc                                 >  2:890272/1‑125 (MQ=255)                                                                                                                 gTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGc                                 <  2:656242/125‑1 (MQ=255)                                                                                                                 gTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGc                                 >  1:656242/1‑125 (MQ=255)                                                                                                                 gTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGc                                 <  1:940983/125‑1 (MQ=255)                                                                                                                 gTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGc                                 <  1:890272/125‑1 (MQ=255)                                                                                                                 gTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATcg                  >  2:359807/1‑140 (MQ=255)                                                                                                                 gTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATcg                  >  1:988811/1‑140 (MQ=255)                                                                                                                 gTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATcg                  >  2:207869/1‑140 (MQ=255)                                                                                                                 gTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATcg                  >  2:686168/1‑140 (MQ=255)                                                                                                                 gTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATcg                  >  1:397761/1‑140 (MQ=255)                                                                                                                 gTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATcg                  >  1:771246/1‑140 (MQ=255)                                                                                                                 gTTTGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATcg                  >  2:330060/1‑140 (MQ=255)                                                                                                                  tttGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATcgc                 >  2:647694/1‑140 (MQ=255)                                                                                                                  tttGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATcgc                 <  2:911640/140‑1 (MQ=255)                                                                                                                   ttGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCTCCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATcgcg                <  2:771246/140‑1 (MQ=255)                                                                                                                    tGCTAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCgcgc               >  1:788651/1‑140 (MQ=255)                                                                                                                       tAAAATCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATc            >  1:365992/1‑140 (MQ=255)                                                                                                                            tCGTTCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGaa       >  1:280670/1‑140 (MQ=255)                                                                                                                               ttCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAgcg    <  2:279611/140‑1 (MQ=255)                                                                                                                               ttCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAgcg    <  2:788651/140‑1 (MQ=255)                                                                                                                               ttCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAgcg    >  2:847753/1‑140 (MQ=255)                                                                                                                               ttCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAgcg    <  1:143774/140‑1 (MQ=255)                                                                                                                               ttCCAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAgcg    <  2:204751/140‑1 (MQ=255)                                                                                                                                 ccAACCGCACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCa  <  1:207869/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     AAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCA  >  NC_000913/3173098‑3173365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑