Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,194,156	A→G	A431A (GCA→GCG)	yqiK →	protein YqiK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,194,156	0	A	G	100.0%	176.7 / NA	50	A431A (GCA→GCG)	yqiK	protein YqiK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/26);  total (24/26)

CCAAAGCGGTTGAACTGACCGTGCGGGCGAAAGCAGAAAAAGAAGCCGCAGAGATGCAGGCGGCGGCTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCACAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGACGGTAT  >  NC_000913/3194035‑3194290                                                                                                                          |                                                                                                                                       ccAAAGCGGTTGAACTGACCGTGCGGGCGAAAGCAGAAAAAGAAGCCGCAGAGATGCAGGCGGCGGCTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGAt                                                                                                                      <  1:732207/140‑1 (MQ=255) ccAAAGCGGTTGAACTGACCGTGCGGGCGAAAGCAGAAAAAGAAGCCGCAGAGATGCAGGCGGCGGCTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGAt                                                                                                                      <  1:235059/140‑1 (MQ=255)   aaaGCGGTTGAACTGACCGTGCGGGCGAAAGCAGAAAAAGAAGCCGCAGAGATGCAGGCGGCGGCTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGc                                                                                                                    <  1:605210/140‑1 (MQ=255)   aaaGCGGTTGAACTGACCGTGCGGGCGAAAGCAGAAAAAGAAGCCGCAGAGATGCAGGCGGCGACTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGc                                                                                                                    <  1:219508/140‑1 (MQ=255)    aaGCGGTTGAACTGACCGTGCGGGCGAAAGCAGAAAAAGAAGCCGCAGAGATGCAGGCGGCGGCTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGcc                                                                                                                   >  2:760994/1‑139 (MQ=255)      gCGGTTGAACTGACCGTGCGGGCGAAAGCAGAAAAAGAAGCCGCAGAGATGCAGGCGGCGGCTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTAt                                                                                                                 <  2:980092/140‑1 (MQ=255)       cGGTTGAACTGACCGTGCGGGCGAAAGCAGAAAAAGAAGCCGCAGAGATGCAGGCGGCGGCTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATc                                                                                                                <  2:1007546/140‑1 (MQ=255)           tGAACTGACCGTGCGGGCGAAAGCAGAAAAAGAAGCCGCAGAGATGCAGGCGGCGGCTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACg                                                                                                            >  1:213013/1‑140 (MQ=255)                  aCCGTGCGGGCGAAAGCAGAAAAAGAAGCCGCAGAGATGCAGGCGGCGGCTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTc                                                                                                     >  2:1026969/1‑140 (MQ=255)                    cGTGCGGGCGAAAGCAGAAAAAGAAGCCGCAGAGATGCAGGCGGCGGCTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCtg                                                                                                   <  2:378134/140‑1 (MQ=255)                              aaggCAGAAAAAGAAGCCGCAGAGATGCAGGCGGCGGCTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAAc                                                                                         <  1:852047/137‑1 (MQ=255)                                     aaaaaGAAGCCGCAGAGATGCAGGCGGCGGCTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCtg                                                                                                   >  2:976089/1‑123 (MQ=255)                                     aaaaaGAAGCCGCAGAGATGCAGGCGGCGGCTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCtg                                                                                                   <  1:976089/123‑1 (MQ=255)                                                    agaTGCAGGCGGCGGCTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGcc                                                                   <  1:227768/140‑1 (MQ=255)                                                           ggcggcggcTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGc                                                            >  2:1021295/1‑140 (MQ=255)                                                            gcggcggcTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCa                                                           >  2:640254/1‑140 (MQ=255)                                                              ggcggcTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAgg                                                         >  2:143177/1‑140 (MQ=255)                                                               gcggcTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGgc                                                        <  2:1029532/140‑1 (MQ=255)                                                                cggcTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCttgt                                                               >  2:277690/1‑132 (MQ=255)                                                                cggcTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCttgt                                                               <  1:277690/132‑1 (MQ=255)                                                                cggcTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGgcg                                                       >  2:622979/1‑140 (MQ=255)                                                                cggcTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGgcg                                                       >  2:590422/1‑140 (MQ=255)                                                                cggcTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGgcg                                                       >  1:510212/1‑140 (MQ=255)                                                                   cTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGctg                                                    <  2:510212/140‑1 (MQ=255)                                                                     aTCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGctgcc                                                  <  2:368729/140‑1 (MQ=255)                                                                     aTCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGctgcc                                                  <  2:213013/140‑1 (MQ=255)                                                                     aTCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGctgcc                                                  <  2:720573/140‑1 (MQ=255)                                                                       cGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGctgcctg                                                <  1:640254/140‑1 (MQ=255)                                                                          tGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCgg                                             <  1:345065/140‑1 (MQ=255)                                                                                aGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGctgcctg                                                <  1:934588/131‑1 (MQ=255)                                                                                aGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGctgcctg                                                >  2:934588/1‑131 (MQ=255)                                                                                aGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTa                                           <  2:284297/136‑1 (MQ=255)                                                                                aGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTa                                           >  1:284297/1‑136 (MQ=255)                                                                                aGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATag                                       >  1:322476/1‑140 (MQ=255)                                                                                 gCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATaga                                      >  1:709379/1‑140 (MQ=255)                                                                                    gAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGaa                                   <  1:1021295/140‑1 (MQ=255)                                                                                           cacGTAAAAAGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCATAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGtt                            >  2:191337/1‑140 (MQ=255)                                                                                                aaaaaGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAg                         <  2:735704/138‑1 (MQ=255)                                                                                                aaaaaGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAg                         >  1:735704/1‑138 (MQ=255)                                                                                                aaaaaGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGcc                       >  2:771193/1‑140 (MQ=255)                                                                                                 aaaaGGGTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCg                      >  1:787217/1‑140 (MQ=255)                                                                                                     gggTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATg                   >  2:959302/1‑139 (MQ=255)                                                                                                     gggTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATg                   <  1:959302/139‑1 (MQ=255)                                                                                                      ggTCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGaa                 <  1:587590/140‑1 (MQ=255)                                                                                                        tCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCGGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGt               >  2:986607/1‑140 (MQ=255)                                                                                                        tCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGt               >  2:298606/1‑140 (MQ=255)                                                                                                        tCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGt               >  1:670874/1‑140 (MQ=255)                                                                                                        tCTGGCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCCCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGt               >  2:671475/1‑140 (MQ=255)                                                                                                            gCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAAt           <  1:143177/140‑1 (MQ=255)                                                                                                            gCGGAAGCAGAAGCGCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCATAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAAt           <  1:191337/140‑1 (MQ=255)                                                                                                               gaagcagaagcgCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGa        <  2:670874/140‑1 (MQ=255)                                                                                                               gaagcagaagcgCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGa        <  1:771193/140‑1 (MQ=255)                                                                                                               gaagcagaagcgCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGa        <  1:1026969/140‑1 (MQ=255)                                                                                                                aagcagaagcgCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGAc       >  1:874390/1‑140 (MQ=255)                                                                                                                aagcagaagcgCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGAc       <  2:874390/140‑1 (MQ=255)                                                                                                                 agcagaagcgCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGACg      <  1:298606/140‑1 (MQ=255)                                                                                                                      aagcgCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGACGGTAt  >  2:154438/1‑139 (MQ=255)                                                                                                                      aagcgCAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGACGGTAt  <  1:154438/139‑1 (MQ=255)                                                                                                                          |                                                                                                                                       CCAAAGCGGTTGAACTGACCGTGCGGGCGAAAGCAGAAAAAGAAGCCGCAGAGATGCAGGCGGCGGCTATCGTTGAGTTAGCCGAAGCTACACGTAAAAAGGGTCTGGCGGAAGCAGAAGCACAACGTGCGCTGAACGATGCTATCAACGTACTTTCTGATGAACAAACCAGCCTTAAATTCAAACTGGCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGACGGTAT  >  NC_000913/3194035‑3194290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑