Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	280,348	A→G	intergenic (‑473/+37)	insA‑2 ← / ← yagB	IS1 protein InsA/orphan antitoxin YagB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	280,348	0	A	G	100.0%	327.5 / NA	90	intergenic (‑473/+37)	insA‑2/yagB	IS1 protein InsA/orphan antitoxin YagB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/46);  total (44/46)

ATCCGGACCGTACTTCTTTATCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCC  >  NC_000913/280216‑280482                                                                                                                                     |                                                                                                                                       aTCCGGACCGTACTTCTTTATCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTAc                                                                                                                                 <  1:894439/140‑1 (MQ=255)          gTACTTCTTTATCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATgg                                                                                                                        >  1:710900/1‑140 (MQ=255)           tACTTCTTTATCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATggg                                                                                                                       >  2:197100/1‑140 (MQ=255)           tACTTCTTTATCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATggg                                                                                                                       >  2:619231/1‑140 (MQ=255)           tACTTCTTTATCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATggg                                                                                                                       <  2:761715/140‑1 (MQ=255)           tACTTCTTTATCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATggg                                                                                                                       <  2:930697/140‑1 (MQ=255)           tACTTCTTTATCCAGGCATAAAGGCTGTGGATGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATggg                                                                                                                       <  2:456387/140‑1 (MQ=255)            aCTTCTTTATCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGc                                                                                                                      >  2:54919/1‑140 (MQ=255)              ttcttTATCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCAc                                                                                                                    <  1:197100/140‑1 (MQ=255)                     tCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATggg                                                                                                                       >  1:356806/1‑130 (MQ=255)                     tCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATggg                                                                                                                       <  2:356806/130‑1 (MQ=255)                     tCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCtt                                                                                                             >  2:493504/1‑140 (MQ=255)                     tCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCtt                                                                                                             >  2:202528/1‑140 (MQ=255)                     tCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCtt                                                                                                             >  1:622239/1‑140 (MQ=255)                         ggCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAg                                                                                                         <  2:589060/140‑1 (MQ=255)                         ggCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAg                                                                                                         <  2:728413/140‑1 (MQ=255)                         ggCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAg                                                                                                         <  2:775493/140‑1 (MQ=255)                          gCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGc                                                                                                        <  1:619231/140‑1 (MQ=255)                          gCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGc                                                                                                        >  1:852458/1‑140 (MQ=255)                                   cTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTaaa                                                                                               <  2:306615/140‑1 (MQ=255)                                   cTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTaaa                                                                                               <  1:272128/140‑1 (MQ=255)                                    tgtgGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAAt                                                                                              >  1:1021204/1‑140 (MQ=255)                                    tgtgGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAAt                                                                                              >  1:193204/1‑140 (MQ=255)                                    tgtgGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAAt                                                                                              >  2:970919/1‑140 (MQ=255)                                      tgggtggTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGa                                                                                            >  1:382564/1‑140 (MQ=255)                                       gggtggTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGAc                                                                                           >  1:737884/1‑140 (MQ=255)                                       gggtggTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGAc                                                                                           >  1:14229/1‑140 (MQ=255)                                        ggtggtGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACt                                                                                          >  2:199416/1‑140 (MQ=255)                                        ggtggtGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACt                                                                                          >  1:795712/1‑140 (MQ=255)                                        ggtggtGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACt                                                                                          <  2:737884/140‑1 (MQ=255)                                           ggtgATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCgg                                                                                       <  2:193204/140‑1 (MQ=255)                                            gtgATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGa                                                                                      >  1:338793/1‑140 (MQ=255)                                            gtgATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGa                                                                                      >  2:135450/1‑140 (MQ=255)                                            gtgATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGa                                                                                      <  2:975780/140‑1 (MQ=255)                                               atatCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGgt                                                                                   <  1:970919/140‑1 (MQ=255)                                                tatCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGgtg                                                                                  <  1:38265/140‑1 (MQ=255)                                                tatCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGgtg                                                                                  >  1:1009584/1‑140 (MQ=255)                                                tatCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGgtg                                                                                  <  1:199416/140‑1 (MQ=255)                                                 atCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTATCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGgtgt                                                                                 <  1:505623/140‑1 (MQ=255)                                                 atCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGgtgt                                                                                 <  1:424154/140‑1 (MQ=255)                                                  tCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTc                                                                                >  1:236634/1‑140 (MQ=255)                                                      gaCGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGtt                                                                            <  1:135450/140‑1 (MQ=255)                                                      gaCGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGtt                                                                            >  1:999522/1‑140 (MQ=255)                                                       aCGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTa                                                                           >  1:61564/1‑140 (MQ=255)                                                           gtTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGa                                                                                      >  1:1043311/1‑125 (MQ=255)                                                           gtTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGa                                                                                      <  2:1043311/125‑1 (MQ=255)                                                            ttGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAAccc                                                                      >  1:495529/1‑140 (MQ=255)                                                            ttGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAAccc                                                                      >  1:932532/1‑140 (MQ=255)                                                               cAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTg                                                                   <  1:757272/140‑1 (MQ=255)                                                                    cTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGAt                                                              >  1:616253/1‑140 (MQ=255)                                                                    cTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGAt                                                              >  2:535947/1‑140 (MQ=255)                                                                       gCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCa                                                           >  2:1081761/1‑140 (MQ=255)                                                                       gCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCa                                                           <  2:622239/140‑1 (MQ=255)                                                                       gCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCa                                                           >  2:978523/1‑140 (MQ=255)                                                                          aCAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCaaaa                                                        <  2:932532/140‑1 (MQ=255)                                                                            aGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAAtt                                                      <  2:1065257/140‑1 (MQ=255)                                                                             gAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAACATTg                                                     <  1:820512/140‑1 (MQ=255)                                                                             gAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTg                                                     <  2:506837/140‑1 (MQ=255)                                                                             gAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTg                                                     <  1:237032/140‑1 (MQ=255)                                                                             gAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGATCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTg                                                     <  2:941181/140‑1 (MQ=255)                                                                              aaTAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGc                                                    <  2:999522/140‑1 (MQ=255)                                                                               aTAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTg                                                     <  2:1044823/138‑1 (MQ=255)                                                                               aTAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTg                                                     >  1:1044823/1‑138 (MQ=255)                                                                                tgaCCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGa                                                  <  2:1003137/138‑1 (MQ=255)                                                                                tAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGa                                                  <  2:322499/140‑1 (MQ=255)                                                                                 aaCCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGat                                                 <  2:1009584/140‑1 (MQ=255)                                                                                  aCCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGata                                                <  2:495529/140‑1 (MQ=255)                                                                                      agaTCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGAtatata                                            >  2:872659/1‑140 (MQ=255)                                                                                      agaTCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGAtatata                                            >  2:750450/1‑140 (MQ=255)                                                                                       gaTCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATatac                                           <  1:535947/140‑1 (MQ=255)                                                                                             caacCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATAccc                                     >  2:396342/1‑140 (MQ=255)                                                                                             caacCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATAccc                                     >  2:320991/1‑140 (MQ=255)                                                                                                 cTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGa                                 <  1:750450/140‑1 (MQ=255)                                                                                                   gTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAGGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACt                               >  2:889440/1‑140 (MQ=255)                                                                                                   gTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACt                               >  1:368391/1‑140 (MQ=255)                                                                                                   gTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACt                               >  1:642285/1‑140 (MQ=255)                                                                                                    tttGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTg                              >  1:996934/1‑140 (MQ=255)                                                                                                    tttGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTg                              >  1:344423/1‑140 (MQ=255)                                                                                                    tttGACTGCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTg                              <  1:623985/140‑1 (MQ=255)                                                                                                           gCTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCg                       >  2:547850/1‑140 (MQ=255)                                                                                                            cTTCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGt                      >  2:611335/1‑140 (MQ=255)                                                                                                              tCAGTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTAt                    >  2:704736/1‑140 (MQ=255)                                                                                                                 gTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCgg                 <  1:1081761/140‑1 (MQ=255)                                                                                                                 gTTTTAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCgg                 <  2:382564/140‑1 (MQ=255)                                                                                                                     tAAACTCTTCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAGGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTc             <  1:889440/140‑1 (MQ=255)                                                                                                                            ttCGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTc      >  2:643827/1‑140 (MQ=255)                                                                                                                              cGGGATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAg    <  1:704736/140‑1 (MQ=255)                                                                                                                                ggATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGcc  <  1:54919/140‑1 (MQ=255)                                                                                                                                ggATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGcc  <  2:795712/140‑1 (MQ=255)                                                                                                                                ggATAGCGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGcc  <  2:656281/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       ATCCGGACCGTACTTCTTTATCCAGGCATAAAGGCTGTGGGTGGTGATATCGAGACGTGTTGCAACGCTGGCAACAGAATAACCGAGATCAACAACCTGTTTGACTGCTTCAGTTTTAAACTCTTCGGGATAACGCTTACCGCTCATGGGCACCTCTCTTTAAGCCATTTTAAATGACTCGGAGGTGTCTGTTAAACCCGTGGCGATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCC  >  NC_000913/280216‑280482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑