Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,299,195	C→T	G74G (GGC→GGT)	yhbO →	protein/nucleic acid deglycase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,299,195	0	C	T	100.0%	201.4 / NA	58	G74G (GGC→GGT)	yhbO	protein/nucleic acid deglycase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/30);  total (28/30)

CACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGC  >  NC_000913/3299061‑3299330                                                                                                                                       |                                                                                                                                        cacGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTc                                                                                                                                    <  2:835837/140‑1 (MQ=255)         gATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGAtt                                                                                                                            <  1:513456/140‑1 (MQ=255)          aTTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTa                                                                                                                           >  1:48456/1‑140 (MQ=255)           ttACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTAt                                                                                                                          <  2:48456/140‑1 (MQ=255)           ttACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTAt                                                                                                                          <  1:998261/140‑1 (MQ=255)              ccATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTg                                                                                                                       >  2:130792/1‑140 (MQ=255)              ccATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTg                                                                                                                       >  1:310747/1‑140 (MQ=255)                  tGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTg                                                                                                                   >  1:666472/1‑140 (MQ=255)                    aaaaaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGTGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTggg                                                                                                                 >  2:618097/1‑140 (MQ=255)                    aaaaaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTggg                                                                                                                 >  2:735439/1‑140 (MQ=255)                    aaaaaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTggg                                                                                                                 <  2:346542/140‑1 (MQ=255)                       aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGAc                                                                                                              <  1:636832/140‑1 (MQ=255)                        aCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACa                                                                                                             <  1:669666/140‑1 (MQ=255)                         cAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCg                                                                                                                     >  1:523440/1‑131 (MQ=255)                         cAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCg                                                                                                                     <  2:523440/131‑1 (MQ=255)                         cAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACaa                                                                                                            <  1:849910/140‑1 (MQ=255)                            gCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCg                                                                                                         <  2:666472/140‑1 (MQ=255)                            gCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCg                                                                                                         >  1:246622/1‑140 (MQ=255)                                          gAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTa                                                                                           >  2:649902/1‑140 (MQ=255)                                                cAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTg                                                                                     <  1:290482/140‑1 (MQ=255)                                                cAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTg                                                                                     <  1:130792/140‑1 (MQ=255)                                                   aaaaGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGAtt                                                                                  <  1:469810/140‑1 (MQ=255)                                                         agaAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGa                                                                            >  2:639795/1‑140 (MQ=255)                                                           aaGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAAt                                                                          >  1:75010/1‑140 (MQ=255)                                                                  cGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCa                                                                   >  2:990853/1‑140 (MQ=255)                                                                      aCCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGaa                                                                           <  2:56846/128‑1 (MQ=255)                                                                      aCCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGaa                                                                           >  1:56846/1‑128 (MQ=255)                                                                       ccATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCg                                                              <  2:75010/140‑1 (MQ=255)                                                                        cATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCgg                                                             <  2:546624/140‑1 (MQ=255)                                                                           cGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGgtgt                                                          >  2:318019/1‑140 (MQ=255)                                                                                 aTCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCa                                                    >  1:721546/1‑140 (MQ=255)                                                                                  tCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCAt                                                   >  1:641935/1‑140 (MQ=255)                                                                                  tCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCAt                                                   <  1:639795/140‑1 (MQ=255)                                                                                    cATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCt                                                 >  2:558289/1‑140 (MQ=255)                                                                                     aTCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGTAAACCGGTGTTTGCCATCTg                                                >  2:2062/1‑140 (MQ=255)                                                                                      tCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGt                                               >  1:283210/1‑140 (MQ=255)                                                                                          tGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACg                                           <  1:318019/140‑1 (MQ=255)                                                                                                gACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGTAAACCGGTGTTTGCCATCTGTCACGGCCCGc                                     <  1:2062/140‑1 (MQ=255)                                                                                                gACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGc                                     <  2:641935/140‑1 (MQ=255)                                                                                                gACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGc                                     <  2:283210/140‑1 (MQ=255)                                                                                                gACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGc                                     <  1:990853/140‑1 (MQ=255)                                                                                                   gCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGt                                  >  1:240772/1‑140 (MQ=255)                                                                                                       gCGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCt                              >  2:874826/1‑140 (MQ=255)                                                                                                        cGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGc                                     >  2:671779/1‑132 (MQ=255)                                                                                                        cGGAGTTTGATGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGc                                     <  1:671779/132‑1 (MQ=255)                                                                                                                  tGCCCTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCg                   >  1:477474/1‑140 (MQ=255)                                                                                                                     ccTGCTGCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGAtg                <  1:874826/140‑1 (MQ=255)                                                                                                                      ctgctgCTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGAtgt               >  2:614553/1‑140 (MQ=255)                                                                                                                       tgctgcTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCt                              >  2:783486/1‑124 (MQ=255)                                                                                                                       tgctgcTACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCt                              <  1:783486/124‑1 (MQ=255)                                                                                                                          tgctACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGAtt           >  2:1018399/1‑140 (MQ=255)                                                                                                                            ctACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCg         <  1:91099/140‑1 (MQ=255)                                                                                                                            ctACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCg         <  1:735439/140‑1 (MQ=255)                                                                                                                            ctACCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCg         <  1:558289/140‑1 (MQ=255)                                                                                                                              aCCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCggg       <  2:456732/140‑1 (MQ=255)                                                                                                                              aCCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCggg       <  2:1036231/140‑1 (MQ=255)                                                                                                                              aCCGGGCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCggg       >  2:71593/1‑140 (MQ=255)                                                                                                                                 gggCGGTCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGgc    >  1:807518/1‑140 (MQ=255)                                                                                                                                  ggcggtCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGgcg   >  1:161426/1‑140 (MQ=255)                                                                                                                                   gcggtCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGa                  <  2:220841/124‑1 (MQ=255)                                                                                                                                   gcggtCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGa                  >  1:220841/1‑124 (MQ=255)                                                                                                                                   gcggtCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGgcgc  >  1:242258/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        CACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGC  >  NC_000913/3299061‑3299330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑