Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,356,124	A→G	E467G (GAG→GGG)	gltB →	glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,356,124	0	A	G	100.0%	265.3 / NA	74	E467G (GAG→GGG)	gltB	glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/38);  total (36/38)

TAAAGAGTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGT  >  NC_000913/3355990‑3356252                                                                                                                                       |                                                                                                                                 taAAGAGTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTgg                                                                                                                             <  1:308185/140‑1 (MQ=255)  aaaGAGTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGa                                                                                                                            <  1:401559/140‑1 (MQ=255)  aaaGAGTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGa                                                                                                                            >  1:569137/1‑140 (MQ=255)      agTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTcc                                                                                                                        >  2:914273/1‑140 (MQ=255)      agTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTcc                                                                                                                        >  1:1063118/1‑140 (MQ=255)       gTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCg                                                                                                                       >  1:134831/1‑140 (MQ=255)       gTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCg                                                                                                                       >  1:266649/1‑140 (MQ=255)       gTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCg                                                                                                                       >  2:926916/1‑140 (MQ=255)        tggatggaGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGt                                                                                                                      <  2:701846/140‑1 (MQ=255)        tggatggaGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGt                                                                                                                      <  1:496953/140‑1 (MQ=255)        tggatggaGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGt                                                                                                                      <  1:712335/140‑1 (MQ=255)         ggctggaGAAAAACGTCCGGCGACTGGTACCGGTTGAAGATCTGGCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTa                                                                                                                     >  2:79190/4‑140 (MQ=255)         ggatggaGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCTGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTa                                                                                                                     >  2:233682/1‑140 (MQ=255)         ggatggaGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTa                                                                                                                     >  1:21445/1‑140 (MQ=255)         ggatggaGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTa                                                                                                                     >  1:660720/1‑140 (MQ=255)          gatggaGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTaa                                                                                                                    >  1:657251/1‑140 (MQ=255)          gatggaGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTaa                                                                                                                    >  2:643992/1‑140 (MQ=255)                  aaaaCGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTa                                                                                                            <  2:497295/140‑1 (MQ=255)                  aaaaCGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTa                                                                                                            <  2:106371/140‑1 (MQ=255)                   aaaCGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTAc                                                                                                           <  2:657251/140‑1 (MQ=255)                         ccgccgACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCg                                                                                                     >  2:45292/1‑140 (MQ=255)                         ccgccgACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCg                                                                                                     >  2:583913/1‑140 (MQ=255)                          cgccgACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGa                                                                                                    >  2:313803/1‑140 (MQ=255)                             cgACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCTGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGaaaa                                                                                                 <  1:233682/140‑1 (MQ=255)                             cgACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGaaaa                                                                                                 <  1:914273/140‑1 (MQ=255)                               aCTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACg                                                                                               >  2:138493/1‑140 (MQ=255)                                        gTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGaaaa                                                                                                 >  1:524135/1‑129 (MQ=255)                                        gTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGaaaa                                                                                                 <  2:524135/129‑1 (MQ=255)                                             aaGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGcggtc                                                                                 >  1:427418/1‑140 (MQ=255)                                             aaGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGcggtc                                                                                 >  2:1021703/1‑140 (MQ=255)                                                aTCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGcggtcggt                                                                              <  1:926916/140‑1 (MQ=255)                                                   tGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCg                                                                           >  2:409968/1‑140 (MQ=255)                                                     cccGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGAt                                                                         >  1:811511/1‑140 (MQ=255)                                                     cccGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGAt                                                                         >  1:1000946/1‑140 (MQ=255)                                                     cccGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGAt                                                                         >  1:865718/1‑140 (MQ=255)                                                        gATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATggg                                                                      <  1:79190/140‑1 (MQ=255)                                                        gATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATggg                                                                      <  2:427418/140‑1 (MQ=255)                                                           gaagaaGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCga                                                                   <  1:409968/140‑1 (MQ=255)                                                                 gTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATAc                                                             <  2:811511/140‑1 (MQ=255)                                                                 gTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATAc                                                             <  2:266649/140‑1 (MQ=255)                                                                    ggTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATAcccc                                                          >  2:147090/1‑140 (MQ=255)                                                                         ccGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCg                                                     >  1:989774/1‑140 (MQ=255)                                                                           gcgAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGcc                                                   <  1:444578/140‑1 (MQ=255)                                                                            cgAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCg                                                  >  2:855372/1‑140 (MQ=255)                                                                             gAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGt                                                 <  2:660720/140‑1 (MQ=255)                                                                             gAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGt                                                 <  1:147090/140‑1 (MQ=255)                                                                             gAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGt                                                 <  2:21445/140‑1 (MQ=255)                                                                              aaCTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGcc                                                   >  1:311481/1‑137 (MQ=255)                                                                              aaCTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGcc                                                   <  2:311481/137‑1 (MQ=255)                                                                              aaCTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTg                                                >  2:365426/1‑140 (MQ=255)                                                                                  ggACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGctct                                            <  2:1063118/140‑1 (MQ=255)                                                                                         gacACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCa                                     >  2:217907/1‑140 (MQ=255)                                                                                           cacGCTTGCCAGCTACCAGAAACAGTTTAACTACGGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGc                                   >  2:961813/1‑140 (MQ=255)                                                                                             cGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCcg                                 >  2:396758/1‑140 (MQ=255)                                                                                             cGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCcg                                 <  1:138493/140‑1 (MQ=255)                                                                                             cGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCcg                                 <  2:569137/140‑1 (MQ=255)                                                                                              gCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCTGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCcgc                                <  2:946923/140‑1 (MQ=255)                                                                                              gCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCcgc                                <  1:583913/140‑1 (MQ=255)                                                                                              gCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCcgc                                >  1:139559/1‑140 (MQ=255)                                                                                               cTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCcgcg                               <  2:139559/140‑1 (MQ=255)                                                                                                ttGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCgcgc                              >  2:1029248/1‑140 (MQ=255)                                                                                                 tGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCa                             <  2:865718/140‑1 (MQ=255)                                                                                                 tGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCa                             <  2:989774/140‑1 (MQ=255)                                                                                                 tGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCa                             <  1:866822/140‑1 (MQ=255)                                                                                                 tGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCa                             <  1:1029248/140‑1 (MQ=255)                                                                                                      gCTACCAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCattatt                        <  1:313803/140‑1 (MQ=255)                                                                                                          ccAGAAACAGTTTAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACg                    >  1:1011509/1‑140 (MQ=255)                                                                                                                    tttAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCg          >  1:23561/1‑140 (MQ=255)                                                                                                                     ttAACTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGc         <  1:537048/140‑1 (MQ=255)                                                                                                                       aaCTACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCca       <  1:1021703/140‑1 (MQ=255)                                                                                                                          tACAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCcagca    <  1:396758/140‑1 (MQ=255)                                                                                                                            cAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGt  <  2:23561/140‑1 (MQ=255)                                                                                                                            cAGCGCGGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGCTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGt  >  1:572033/1‑140 (MQ=255)                                                                                                                                 cgGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCGGCAGt  <  1:97790/135‑1 (MQ=255)                                                                                                                                 cgGAAGGGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCGGCAGt  >  2:97790/1‑135 (MQ=255)                                                                                                                                       |                                                                                                                                 TAAAGAGTGGATGGAGAAAAACGTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTGGACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGT  >  NC_000913/3355990‑3356252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑