Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,377,373	A→G	G16G (GGT→GGC)	sspA ←	stringent starvation protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,377,373	0	A	G	100.0%	198.2 / NA	55	G16G (GGT→GGC)	sspA	stringent starvation protein A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/27);  total (28/27)

TCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGACCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTACCCAATAAGGAACGA  >  NC_000913/3377240‑3377505                                                                                                                                      |                                                                                                                                     tCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGTCGGaa                                                                                                                                >  1:538384/1‑140 (MQ=255) tCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGaa                                                                                                                                >  1:335472/1‑140 (MQ=255) tCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGaa                                                                                                                                >  1:482744/1‑140 (MQ=255) tCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGaa                                                                                                                                >  1:505342/1‑140 (MQ=255) tCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGaa                                                                                                                                >  1:510802/1‑140 (MQ=255) tCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGaa                                                                                                                                >  1:638498/1‑140 (MQ=255)   ggAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGaaaa                                                                                                                              <  2:388707/140‑1 (MQ=255)    gAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGaa                                                                                                                                >  2:734276/1‑137 (MQ=255)    gAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGaa                                                                                                                                <  1:734276/137‑1 (MQ=255)    gAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAAc                                                                                                                             <  2:638498/140‑1 (MQ=255)     aaCGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACa                                                                                                                            <  2:335472/140‑1 (MQ=255)          tctGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTc                                                                                                                       <  1:989162/140‑1 (MQ=255)               ttCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTAc                                                                                                                  <  2:510802/140‑1 (MQ=255)                   ggTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGaa                                                                                                              <  2:1009869/140‑1 (MQ=255)                                   aTCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCTAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGa                                                                                              <  2:971323/140‑1 (MQ=255)                                    tCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGAc                                                                                             >  1:1042300/1‑140 (MQ=255)                                        gAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGcc                                                                                         <  2:482744/140‑1 (MQ=255)                                        gAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGcc                                                                                         <  2:1042300/140‑1 (MQ=255)                                             ggATTGTCCTTTTCCACGTGTTCAATCTCGAAGCTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAGCGTTTGTTGGCAGCGACAGCCATGaa                                                                                    <  2:1045322/140‑1 (MQ=255)                                              gATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGTCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGaaa                                                                                   <  2:538384/140‑1 (MQ=255)                                                         tCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGta                                                                        >  2:881934/1‑140 (MQ=255)                                                             cGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTc                                                                              <  2:883264/130‑1 (MQ=255)                                                             cGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTc                                                                              >  1:883264/1‑130 (MQ=255)                                                             cGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGt                                                                    >  1:1009479/1‑140 (MQ=255)                                                             cGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGt                                                                    >  2:235936/1‑140 (MQ=255)                                                             cGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGt                                                                    >  2:650350/1‑140 (MQ=255)                                                                gtTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAg                                                                 >  2:102592/1‑140 (MQ=255)                                                                         cGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTAc                                                        >  1:551947/1‑140 (MQ=255)                                                                               ttACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTAc                                                  <  1:881934/140‑1 (MQ=255)                                                                                  cacCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAg                                               >  2:357236/1‑140 (MQ=255)                                                                                    ccTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGcc                                             <  2:551947/140‑1 (MQ=255)                                                                                       ttCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGccacc                                          >  1:8254/1‑140 (MQ=255)                                                                                        tctcAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGccacca                                         >  2:39915/1‑140 (MQ=255)                                                                                             gccagcACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTgg                                    >  2:947358/1‑140 (MQ=255)                                                                                             gccagcACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTgg                                    <  2:1009479/140‑1 (MQ=255)                                                                                                 gcACAATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAg                                >  2:615865/1‑140 (MQ=255)                                                                                                  cacaATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGt                               >  1:901916/1‑140 (MQ=255)                                                                                                  cacaATGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGt                               >  2:1076951/1‑140 (MQ=255)                                                                                                       tGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGaa                          >  2:634726/1‑140 (MQ=255)                                                                                                       tGCGGACCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGaa                          >  1:747569/1‑140 (MQ=255)                                                                                                            aCCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTtgt                     >  1:678559/1‑140 (MQ=255)                                                                                                            aCCTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTtgt                     >  1:774149/1‑140 (MQ=255)                                                                                                             ccTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTtgtg                    <  2:678559/140‑1 (MQ=255)                                                                                                             ccTGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTtgtg                    <  2:901916/140‑1 (MQ=255)                                                                                                               tGATGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGtt                  <  1:235936/140‑1 (MQ=255)                                                                                                                 aTGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTAc                >  2:199408/1‑140 (MQ=255)                                                                                                                  tGGCTATAGATGTCTGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTAcc               <  1:439427/140‑1 (MQ=255)                                                                                                                  tGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTAcc               <  2:505342/140‑1 (MQ=255)                                                                                                                  tGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTAcc               <  2:480809/140‑1 (MQ=255)                                                                                                                  tGGCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTAcc               <  1:1076951/140‑1 (MQ=255)                                                                                                                    gCTATAGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTACCCa             <  2:774149/140‑1 (MQ=255)                                                                                                                      tataGATGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTACCCAAt           >  1:350008/1‑140 (MQ=255)                                                                                                                           aTGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTACCCAATAAGGa      <  1:199408/140‑1 (MQ=255)                                                                                                                            tGTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTACCCAATAAGGaa     <  2:350008/140‑1 (MQ=255)                                                                                                                             gTCAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTACCCAATAAGGAAc    <  1:634726/140‑1 (MQ=255)                                                                                                                               cAGTAGGGCCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTACCCAATAAGGAACGa  <  2:762055/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     TCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGACCGGAAAACAGCGTCATTACCGAACGTTTGTTGGCAGCGACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTACCCAATAAGGAACGA  >  NC_000913/3377240‑3377505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑