Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,422,311	T→C	G329G (GGT→GGC)	yhdY →	putative ABC transporter membrane subunit YhdY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,422,311	0	T	C	100.0%	253.1 / NA	70	G329G (GGT→GGC)	yhdY	putative ABC transporter membrane subunit YhdY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/31);  total (39/31)

TGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACACCGCATTGAGGACACTA  >  NC_000913/3422178‑3422436                                                                                                                                      |                                                                                                                              tGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCg                                                                                                                         >  1:640312/1‑140 (MQ=255) tGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCg                                                                                                                         >  2:521187/1‑140 (MQ=255)    aGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACg                                                                                                                      <  1:933167/140‑1 (MQ=255)     gCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACgg                                                                                                                     <  2:122252/140‑1 (MQ=255)       tGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGaa                                                                                                                   >  1:336637/1‑140 (MQ=255)         gTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAgg                                                                                                                 >  1:261758/1‑140 (MQ=255)                   ggCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGa                                                                                                                    <  2:221181/127‑1 (MQ=255)                   ggCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGa                                                                                                                    >  1:221181/1‑127 (MQ=255)                       ggTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTcgccg                                                                                                   <  2:640312/140‑1 (MQ=255)                       ggTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTcgccg                                                                                                   <  2:336637/140‑1 (MQ=255)                        gTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTcgccgc                                                                                                  >  2:873460/1‑140 (MQ=255)                        gTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTcgccgc                                                                                                  >  2:36211/1‑140 (MQ=255)                            aTACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTTGCCGCGCTg                                                                                              <  1:189488/140‑1 (MQ=255)                            aTACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTg                                                                                              <  1:808985/140‑1 (MQ=255)                              aCCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGAt                                                                                            >  2:761946/1‑140 (MQ=255)                                  tcatcGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCcgcg                                                                                                 <  1:1022628/131‑1 (MQ=255)                                  tcatcGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCcgcg                                                                                                 >  2:1022628/1‑131 (MQ=255)                                  tcatcGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTg                                                                                              >  1:914526/1‑134 (MQ=255)                                  tcatcGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTg                                                                                              <  2:914526/134‑1 (MQ=255)                                  tcatcGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTAc                                                                                        >  1:930354/1‑140 (MQ=255)                                  tcatcGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTAc                                                                                        >  1:154040/1‑140 (MQ=255)                                   catcGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTg                                                                                              <  1:369466/133‑1 (MQ=255)                                   catcGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTg                                                                                              >  2:369466/1‑133 (MQ=255)                                   catcGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACt                                                                                       >  1:713385/1‑140 (MQ=255)                                          ctctTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGAtctt                                                                                >  2:366668/1‑140 (MQ=255)                                             ttCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTg                                                                             >  1:715158/1‑140 (MQ=255)                                             ttCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTg                                                                             <  2:200642/140‑1 (MQ=255)                                                     tACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCa                                                                     >  2:79686/1‑140 (MQ=255)                                                       ccAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATg                                                                   <  2:599444/140‑1 (MQ=255)                                                       ccAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATg                                                                   >  1:599444/1‑140 (MQ=255)                                                       ccAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATg                                                                   >  1:959552/1‑140 (MQ=255)                                                          gCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTcg                                                                >  2:560664/1‑140 (MQ=255)                                                          gCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTcg                                                                <  2:341989/140‑1 (MQ=255)                                                           ccTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTcgc                                                               >  1:453173/1‑140 (MQ=255)                                                            cTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTcgcg                                                              <  1:366668/140‑1 (MQ=255)                                                              ggTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCa                                                                        <  1:890640/128‑1 (MQ=255)                                                              ggTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCa                                                                        >  2:890640/1‑128 (MQ=255)                                                              ggTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCt                                                            <  1:521187/140‑1 (MQ=255)                                                               gTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCta                                                           >  2:524090/1‑140 (MQ=255)                                                                    cATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGcc                                                      >  1:45364/1‑140 (MQ=255)                                                                     aTTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCa                                                     <  2:45364/140‑1 (MQ=255)                                                                         tCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTAt                                                 >  2:447520/1‑140 (MQ=255)                                                                         tCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTAt                                                 >  2:917687/1‑140 (MQ=255)                                                                         tCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATATCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTAt                                                 >  1:854442/1‑140 (MQ=255)                                                                            ggTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTTTGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTg                                              >  1:1086820/1‑140 (MQ=255)                                                                              ttgttCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGa                                            >  1:1015920/1‑140 (MQ=255)                                                                                 ttCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGaaaa                                         >  2:952345/1‑140 (MQ=255)                                                                                 ttCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGaaaa                                         >  1:571754/1‑140 (MQ=255)                                                                                      tCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGtt                                    >  2:729626/1‑140 (MQ=255)                                                                                             aGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAacac                             <  2:154040/140‑1 (MQ=255)                                                                                             aGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAacac                             <  1:873460/140‑1 (MQ=255)                                                                                             aGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAacac                             <  2:715158/140‑1 (MQ=255)                                                                                                  cGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGc                        >  1:72060/1‑140 (MQ=255)                                                                                                    ttCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGt                      >  2:378112/1‑140 (MQ=255)                                                                                                    ttCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGt                      >  1:343034/1‑140 (MQ=255)                                                                                                       agcagGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTaca                   <  1:560664/140‑1 (MQ=255)                                                                                                       agcagGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTaca                   <  2:713385/140‑1 (MQ=255)                                                                                                        gcagGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTacac                  <  1:447520/140‑1 (MQ=255)                                                                                                        gcagGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTacac                  <  1:917687/140‑1 (MQ=255)                                                                                                         cagGCAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACAcc                 >  2:952469/1‑140 (MQ=255)                                                                                                         cagGCAACCGTTGATCCCGCCTGGTTGGGCATATCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACAcc                 <  2:854442/140‑1 (MQ=255)                                                                                                             cAACCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACACCGCAt             <  1:683211/140‑1 (MQ=255)                                                                                                              aaCCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACACCGCAtt            <  1:952469/140‑1 (MQ=255)                                                                                                              aaCCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACACCGCAtt            <  1:729626/140‑1 (MQ=255)                                                                                                              aaCCGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACACCGCAtt            <  1:36211/140‑1 (MQ=255)                                                                                                                ccGTTGATCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACACCGCATTGa          >  2:872431/1‑140 (MQ=255)                                                                                                                      aTCCCGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACACCGCATTGAGGacac    >  1:198731/1‑140 (MQ=255)                                                                                                                        cccGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTaca                   >  2:1055233/1‑123 (MQ=255)                                                                                                                        cccGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTaca                   <  1:1055233/123‑1 (MQ=255)                                                                                                                        cccGCCTGGTTGGGCATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACACCGCATTGAGGACACTa  <  1:952345/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                              TGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCGCACTCTTCAAAGATACCAGCCTGGTGATCATTATCGGGTTGTTCGATCTTTTCAGTAGCGTTCAGCAGGCAACCGTTGATCCCGCCTGGTTGGGTATGTCGACGGAAGGGTATGTTTTCGCCGCGCTGATCTACTGGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACACCGCATTGAGGACACTA  >  NC_000913/3422178‑3422436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑