Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,913,854	C→T	W449* (TGG→TGA)	glmU ←	fused N‑acetylglucosamine‑1‑phosphate uridyltransferase and glucosamine‑1‑phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,913,854	0	C	T	100.0%	290.4 / NA	81	W449* (TGG→TGA)	glmU	fused N‑acetylglucosamine‑1‑phosphate uridyltransferase and glucosamine‑1‑phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/38);  total (43/38)

TTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAA  >  NC_000913/3913718‑3913989                                                                                                                                         |                                                                                                                                        ttttCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAg                                                                                                                                      <  1:33579/140‑1 (MQ=255)   ttCGGGCGCCCCTAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGcc                                                                                                                                    <  1:696478/140‑1 (MQ=255)   ttCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGcc                                                                                                                                    <  1:968523/140‑1 (MQ=255)               gAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGt                                                                                                                        <  2:582406/140‑1 (MQ=255)                  ccTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTg                                                                                                                     <  2:1042460/140‑1 (MQ=255)                      gTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGc                                                                                                                 >  2:822965/1‑140 (MQ=255)                      gTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGc                                                                                                                 >  2:623535/1‑140 (MQ=255)                            agTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACgg                                                                                                           >  1:1042635/1‑140 (MQ=255)                            agTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACgg                                                                                                           >  1:492932/1‑140 (MQ=255)                            agTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAATCTGCGGCACACgg                                                                                                           >  1:214212/1‑140 (MQ=255)                              tGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCt                                                                                                         >  2:607909/1‑140 (MQ=255)                              tGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCt                                                                                                         >  2:106243/1‑140 (MQ=255)                               ggggTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTg                                                                                                        >  1:96365/1‑140 (MQ=255)                                gggTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGa                                                                                                       >  2:512179/1‑140 (MQ=255)                                gggTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGa                                                                                                       <  1:822965/140‑1 (MQ=255)                                 ggTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGAt                                                                                                      <  1:512179/140‑1 (MQ=255)                                                 aCTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGa                                                                                                       >  2:456942/1‑123 (MQ=255)                                                 aCTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGa                                                                                                       <  1:456942/123‑1 (MQ=255)                                                 aCTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCg                                                                                      >  1:157306/1‑140 (MQ=255)                                                 aCTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCg                                                                                      >  1:481338/1‑140 (MQ=255)                                                 aCTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCg                                                                                      >  2:962105/1‑140 (MQ=255)                                                  cTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGc                                                                                     <  2:96365/140‑1 (MQ=255)                                                       tgtgGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGcc                                                                                    >  2:165869/1‑136 (MQ=255)                                                       tgtgGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGcc                                                                                    <  1:165869/136‑1 (MQ=255)                                                       tgtgGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACa                                                                                <  1:898832/140‑1 (MQ=255)                                                        gtgGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACAt                                                                               >  1:269844/1‑140 (MQ=255)                                                          gggagggaGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTa                                                                             <  2:481338/140‑1 (MQ=255)                                                               ggagATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGt                                                                        >  1:523517/1‑140 (MQ=255)                                                               ggagATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGt                                                                        >  1:73008/1‑140 (MQ=255)                                                               ggagATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGt                                                                        <  1:427239/140‑1 (MQ=255)                                                               ggagATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGt                                                                        >  2:775532/1‑140 (MQ=255)                                                               ggagATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGt                                                                        >  2:47141/1‑140 (MQ=255)                                                                 agaTTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTca                                                                      <  1:392022/140‑1 (MQ=255)                                                                  gaTTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGcc                                                                                    >  2:426524/1‑125 (MQ=255)                                                                  gaTTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGcc                                                                                    <  1:426524/125‑1 (MQ=255)                                                                   aTTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTca                                                                      >  1:104845/1‑138 (MQ=255)                                                                   aTTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTca                                                                      <  2:104845/138‑1 (MQ=255)                                                                        gTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCTCACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGt                                                               <  1:34804/140‑1 (MQ=255)                                                                             ctcctcATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCg                                                          >  2:1030760/1‑140 (MQ=255)                                                                                acagtCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCgcag                                                       >  1:1034327/5‑140 (MQ=255)                                                                                 tcATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGt                                                                  <  1:64679/128‑1 (MQ=255)                                                                                 tcATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGt                                                                  >  2:64679/1‑128 (MQ=255)                                                                                  cATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCgcagca                                                     <  1:962105/140‑1 (MQ=255)                                                                                  cATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATACATTTTCGCCGACATTACGCGTCACAGTTGTACCCgcagca                                                     <  1:607909/140‑1 (MQ=255)                                                                                   aTCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGATGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCaa                                                    >  2:27806/1‑140 (MQ=255)                                                                                       cATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGt                                                >  2:293564/1‑140 (MQ=255)                                                                                        aTGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTc                                               >  1:805668/1‑140 (MQ=255)                                                                                        aTGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTc                                               >  2:814459/1‑140 (MQ=255)                                                                                          gtgACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTcgc                                             >  1:194176/1‑140 (MQ=255)                                                                                          gtgACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTcgc                                             >  2:508520/1‑140 (MQ=255)                                                                                           tgACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTcgcg                                            <  2:805668/140‑1 (MQ=255)                                                                                             aCCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGcc                                          <  1:508520/140‑1 (MQ=255)                                                                                             aCCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGcc                                          <  2:1034327/140‑1 (MQ=255)                                                                                              ccGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCt                                         <  1:106243/140‑1 (MQ=255)                                                                                               cGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCtt                                        >  1:148492/1‑140 (MQ=255)                                                                                               cGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCtt                                        >  1:201102/1‑140 (MQ=255)                                                                                                 ggTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTg                                      >  1:256573/1‑140 (MQ=255)                                                                                                   ttAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGcc                                    >  2:1047930/1‑140 (MQ=255)                                                                                                   ttAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGcc                                    >  2:672686/1‑140 (MQ=255)                                                                                                    tAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCt                                   <  1:672686/140‑1 (MQ=255)                                                                                                    tAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCt                                   <  1:1047930/140‑1 (MQ=255)                                                                                                     aGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGcc                                          >  2:97159/1‑132 (MQ=255)                                                                                                     aGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGcc                                          <  1:97159/132‑1 (MQ=255)                                                                                                     aGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTa                                  >  2:695462/1‑140 (MQ=255)                                                                                                      gCCGGCCAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTAc                                 >  2:795227/1‑140 (MQ=255)                                                                                                           ccAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGcc                                          <  1:953437/126‑1 (MQ=255)                                                                                                           ccAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTa                            >  2:589210/1‑140 (MQ=255)                                                                                                           ccAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTa                            >  2:87707/1‑140 (MQ=255)                                                                                                           ccAGAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGAGAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGcc                                          >  2:953437/1‑126 (MQ=255)                                                                                                              gAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCg                         <  1:589210/140‑1 (MQ=255)                                                                                                              gAATCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGATGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCg                         <  1:27806/140‑1 (MQ=255)                                                                                                               aaTCACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCgg                        >  1:223625/1‑140 (MQ=255)                                                                                                                  cACTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGc                     <  2:910853/140‑1 (MQ=255)                                                                                                                    cTTTTTCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGcca                   <  2:577017/140‑1 (MQ=255)                                                                                                                     tttttCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGccac                  <  2:201102/140‑1 (MQ=255)                                                                                                                     tttttCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGccac                  <  1:623535/140‑1 (MQ=255)                                                                                                                         tCTTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGACACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGc              >  1:567465/1‑140 (MQ=255)                                                                                                                           tttACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTg            <  2:269844/140‑1 (MQ=255)                                                                                                                                   acgacgTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCgg    <  2:641252/140‑1 (MQ=255)                                                                                                                                   acgacgTCAGCCTTCTTTCTGAATCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCgg    <  2:214212/140‑1 (MQ=255)                                                                                                                                     gacgTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGaa  <  1:420888/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAA  >  NC_000913/3913718‑3913989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑