Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,068,294	A→G	V328A (GTG→GCG)	yihQ ←	sulfoquinovosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,068,294	0	A	G	100.0%	274.1 / NA	75	V328A (GTG→GCG)	yihQ	sulfoquinovosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/34);  total (41/34)

GCCGCCGTAAAACTCGCCAAACTCCACCAGATAGTCACCGCCAGAGGCATCTTTTGCCAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCACGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCAGATGC  >  NC_000913/4068158‑4068426                                                                                                                                         |                                                                                                                                     gccgccGTAAAACTCGCCAAACTCCACCAGATAGTCACCGCCAGAGGCATCTTTTGCCAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTgcgcgc                                                                                                                                   <  2:799683/140‑1 (MQ=255)     ccgTAAAACTCGCCAAACTCCACCAGATAGTCACCGCCAGAGGCATCTTTTGCCAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCcctc                                                                                                                               >  1:90707/1‑140 (MQ=255)             cTCGCCAAACTCCACCAGATAGTCACCGCCAGAGGCATCTTTTGCCAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTcc                                                                                                                       <  2:1054072/140‑1 (MQ=255)                   aaaCTCCACCAGATAGTCACCGCCAGAGGCATCTTTTGCCAGATAGCCGTGTTGTACCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCt                                                                                                                 <  2:618906/140‑1 (MQ=255)                                tAGTCACCGCCAGAGGCATCTTTTGCCAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATc                                                                                                    <  1:731182/140‑1 (MQ=255)                                   tCACCGCCAGAGGCATCTTTTGCCAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGt                                                                                                         >  2:58056/1‑132 (MQ=255)                                   tCACCGCCAGAGGCATCTTTTGCCAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGt                                                                                                         <  1:58056/132‑1 (MQ=255)                                   tCACCGCCAGAGGCATCTTTTGCCAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTACACTGCTTAATGCGTGAATCCAg                                                                                                 >  1:403403/1‑140 (MQ=255)                                      ccGCCAGAGGCATCTTTTGCCAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTg                                                                                              >  1:639515/1‑140 (MQ=255)                                        gCCAGAGGCATCTTTTGCCAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCg                                                                                            >  2:171235/1‑140 (MQ=255)                                         ccAGAGGCATCTTTTGCCAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCgg                                                                                           >  1:570516/1‑140 (MQ=255)                                         ccAGAGGCATCTTTTGCCAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCgg                                                                                           >  2:844054/1‑140 (MQ=255)                                          cAGAGGCATCTTTTGCCAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCggg                                                                                          <  1:554012/140‑1 (MQ=255)                                          cAGAGGCATCTTTTGCCAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCggg                                                                                          <  1:647290/140‑1 (MQ=255)                                             agGCATCTTTTGCCAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAg                                                                                       >  1:193962/1‑140 (MQ=255)                                                         ccAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCg                                                                                 <  1:395725/134‑1 (MQ=255)                                                         ccAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCg                                                                                 >  2:395725/1‑134 (MQ=255)                                                          cAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTcc                                                                          <  2:193962/140‑1 (MQ=255)                                                              tAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACtt                                                                      >  2:43668/1‑140 (MQ=255)                                                               aGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTc                                                                     <  2:90707/140‑1 (MQ=255)                                                               aGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTc                                                                     <  2:639515/140‑1 (MQ=255)                                                               aGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTc                                                                     <  1:984263/140‑1 (MQ=255)                                                                    tgttgtGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGtt                                                                >  1:600613/1‑140 (MQ=255)                                                                     gttgtGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTc                                                               <  1:683397/140‑1 (MQ=255)                                                                       tgtgCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCca                                                             >  1:914052/1‑140 (MQ=255)                                                                         tgCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATA‑GCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTc                                                                     <  1:149707/129‑1 (MQ=255)                                                                         tgCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATA‑GCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTc                                                                     >  2:149707/1‑129 (MQ=255)                                                                          gCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACAt                                                          <  1:370036/140‑1 (MQ=255)                                                                           ccGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATc                                                         >  1:190741/1‑140 (MQ=255)                                                                                tcttcGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTTCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCAcgcg                                                    >  1:393301/1‑140 (MQ=255)                                                                                tcttcGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCAcgcg                                                    >  1:579210/1‑140 (MQ=255)                                                                                tcttcGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCAcgcg                                                    <  1:538442/140‑1 (MQ=255)                                                                                tcttcGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCAcgcg                                                    >  1:302734/1‑140 (MQ=255)                                                                                tcttcGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTACACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCAcgcg                                                    <  2:403403/140‑1 (MQ=255)                                                                                 cttcGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGt                                                   >  1:971707/1‑140 (MQ=255)                                                                                   tcGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGttt                                                 >  1:265237/1‑140 (MQ=255)                                                                                         agaTCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCaaa                                           <  2:570516/140‑1 (MQ=255)                                                                                          gaTCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCaaaa                                          <  1:583116/140‑1 (MQ=255)                                                                                           aTCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCAcgcg                                                    <  2:655654/129‑1 (MQ=255)                                                                                           aTCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCAcgcg                                                    >  1:655654/1‑129 (MQ=255)                                                                                           aTCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAag                                         >  1:864476/1‑140 (MQ=255)                                                                                           aTCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAag                                         <  2:939707/140‑1 (MQ=255)                                                                                               ttATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTTCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGt                                     <  2:393301/140‑1 (MQ=255)                                                                                               ttATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGt                                     <  1:8038/140‑1 (MQ=255)                                                                                               ttATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGt                                     <  1:779883/140‑1 (MQ=255)                                                                                               ttATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGt                                     <  2:175397/140‑1 (MQ=255)                                                                                                     cTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGc                                               <  2:987890/124‑1 (MQ=255)                                                                                                     cTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGc                                               >  1:987890/1‑124 (MQ=255)                                                                                                     cTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATAc                                <  2:194554/139‑1 (MQ=255)                                                                                                     cTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATAc                                >  1:194554/1‑139 (MQ=255)                                                                                                     cTGGCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACg                               >  1:149830/1‑140 (MQ=255)                                                                                                        gCAACATACGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAAt                            >  1:327867/1‑140 (MQ=255)                                                                                                               aCGGGTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGAc                     <  2:834282/140‑1 (MQ=255)                                                                                                                 gggTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCa                   <  2:634720/140‑1 (MQ=255)                                                                                                                  ggTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAg                  >  2:1031331/1‑140 (MQ=255)                                                                                                                  ggTTGATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAg                  >  1:445907/1‑140 (MQ=255)                                                                                                                      gATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCt              <  1:171235/140‑1 (MQ=255)                                                                                                                      gATATAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCt              >  1:583134/1‑140 (MQ=255)                                                                                                                       atatAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTg             >  2:532004/1‑140 (MQ=255)                                                                                                                       atatAGGCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTg             >  1:684391/1‑140 (MQ=255)                                                                                                                            ggCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGccc        <  2:579210/140‑1 (MQ=255)                                                                                                                             gCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCt              <  1:375782/133‑1 (MQ=255)                                                                                                                             gCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCt              >  2:375782/1‑133 (MQ=255)                                                                                                                             gCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCa       >  2:185641/1‑140 (MQ=255)                                                                                                                             gCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCa       >  1:589057/1‑140 (MQ=255)                                                                                                                             gCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCa       >  2:68129/1‑140 (MQ=255)                                                                                                                             gCCAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTATTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCa       >  1:950627/1‑140 (MQ=255)                                                                                                                               cAGGAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCAGa     <  1:68129/140‑1 (MQ=255)                                                                                                                                 ggAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCAGATg   <  2:265237/140‑1 (MQ=255)                                                                                                                                 ggAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCAGATg   <  2:864476/140‑1 (MQ=255)                                                                                                                                 ggAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCAGATg   >  2:24541/1‑140 (MQ=255)                                                                                                                                 ggAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCAGATg   >  2:94524/1‑140 (MQ=255)                                                                                                                                  gAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCAGATGc  >  2:238164/1‑140 (MQ=255)                                                                                                                                  gAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCAGATGc  >  2:148996/1‑140 (MQ=255)                                                                                                                                  gAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAAGACCGGACCAGTCCTGCGCCCAGATGc  >  1:758818/1‑140 (MQ=255)                                                                                                                                  gAACTGCGCGCCCTCCTGATTCCACTGCTTAATGCATGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCAGATGc  >  2:561663/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     GCCGCCGTAAAACTCGCCAAACTCCACCAGATAGTCACCGCCAGAGGCATCTTTTGCCAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCACGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCAGATGC  >  NC_000913/4068158‑4068426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑