Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,424,836	G→A	intergenic (‑45/‑282)	yjfY ← / → rpsF	DUF1471 domain‑containing protein YjfY/30S ribosomal subunit protein S6

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,424,836	0	G	A	100.0%	269.7 / NA	75	intergenic (‑45/‑282)	yjfY/rpsF	DUF1471 domain‑containing protein YjfY/30S ribosomal subunit protein S6
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/33);  total (42/33)

TGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGTGATGAACTTCAAATCAGCGTGTTAGAGGTTAATTGCGAAAGGGG  >  NC_000913/4424704‑4424969                                                                                                                                     |                                                                                                                                       tGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTGTTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAAtcttct                                                                                                                                 >  2:958129/1‑140 (MQ=255) tGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAAtcttct                                                                                                                                 >  2:196188/1‑140 (MQ=255)  ggCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTg                                                                                                                                >  2:197980/1‑140 (MQ=255)  ggCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTg                                                                                                                                >  2:127464/1‑140 (MQ=255)  ggCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTg                                                                                                                                >  1:612480/1‑140 (MQ=255)  ggCCTGGCGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTATCAAAAGCACAGCCAGAGTTGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTg                                                                                                                                >  2:1007482/1‑140 (MQ=255)     cTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGAtt                                                                                                                             >  1:465076/1‑140 (MQ=255)       ggtggtTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGAttta                                                                                                                           >  1:641668/1‑140 (MQ=255)        gtggtTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTTGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGAtttaa                                                                                                                          >  2:582997/1‑140 (MQ=255)        gtggtTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGAtttaa                                                                                                                          >  2:580534/1‑140 (MQ=255)             tattaATTTCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGAtttaatttta                                                                                                                     >  2:638080/1‑140 (MQ=255)              attaATTTCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGAtttaattttaa                                                                                                                    >  2:702002/1‑140 (MQ=255)                taATTTCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATc                                                                                                                  <  1:264347/140‑1 (MQ=255)                  aTTTCAATGGCTGCCCATGTATTTGCACTTAGCAATAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCaa                                                                                                                <  2:746692/140‑1 (MQ=255)                  aTTTCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCaa                                                                                                                <  1:196188/140‑1 (MQ=255)                   tttCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAAt                                                                                                               <  2:612480/140‑1 (MQ=255)                   tttCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAAt                                                                                                               >  2:614783/1‑140 (MQ=255)                   tttCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAAt                                                                                                               >  2:89243/1‑140 (MQ=255)                   tttCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAAt                                                                                                               <  2:641668/140‑1 (MQ=255)                     tCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGa                                                                                                             >  2:172869/1‑140 (MQ=255)                     tCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGa                                                                                                             >  1:531147/1‑140 (MQ=255)                        aTGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATaa                                                                                                          <  1:846363/140‑1 (MQ=255)                                    gTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATgg                                                                                              <  2:465680/140‑1 (MQ=255)                                     tATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGt                                                                                             <  2:911660/140‑1 (MQ=255)                                        ttGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGAc                                                                                          <  1:702002/140‑1 (MQ=255)                                         tGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGATCATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGAcc                                                                                         >  1:5799/1‑140 (MQ=255)                                         tGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGAcc                                                                                         >  1:138805/1‑140 (MQ=255)                                             cTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGAcc                                                                                         <  1:241253/136‑1 (MQ=255)                                             cTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGAcc                                                                                         >  2:241253/1‑136 (MQ=255)                                               tAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGAc                                                                                          >  2:947419/1‑133 (MQ=255)                                               tAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGAc                                                                                          <  1:947419/133‑1 (MQ=255)                                               tAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCt                                                                                   >  1:294675/1‑140 (MQ=255)                                               tAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCt                                                                                   >  2:270646/1‑140 (MQ=255)                                                aGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATg                                                                                               >  1:31569/1‑127 (MQ=255)                                                aGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATg                                                                                               <  2:31569/127‑1 (MQ=255)                                                 gCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGa                                                                                 <  1:580534/140‑1 (MQ=255)                                                        cacaGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAgtt                                                                          >  1:126310/1‑140 (MQ=255)                                                          caGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAgttgt                                                                        >  1:958099/1‑140 (MQ=255)                                                           aGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAgttgtt                                                                       <  2:488748/140‑1 (MQ=255)                                                           aGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAgttgtt                                                                       <  1:127013/140‑1 (MQ=255)                                                                 aaGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑aaaaa                                                                >  1:835257/1‑140 (MQ=255)                                                                   gggCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑aaaaaaa                                                              <  2:958099/140‑1 (MQ=255)                                                                   gggCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑aaaaaaa                                                              <  2:919740/140‑1 (MQ=255)                                                                     gCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAaca                                                            <  1:24595/140‑1 (MQ=255)                                                                     gCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAaca                                                            <  2:531147/140‑1 (MQ=255)                                                                      cTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAacac                                                           >  2:850505/1‑140 (MQ=255)                                                                       tAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACAcc                                                          >  1:825609/1‑140 (MQ=255)                                                                         aaaCACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCat                                                        >  1:318347/1‑140 (MQ=255)                                                                            cacGACTGATCATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCatgat                                                     <  2:5799/140‑1 (MQ=255)                                                                               gACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGaa                                                  >  1:640243/1‑140 (MQ=255)                                                                               gACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGaa                                                  >  1:942023/1‑140 (MQ=255)                                                                               gACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGaa                                                  <  1:127464/140‑1 (MQ=255)                                                                                aCTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAg                                                 >  1:813850/1‑140 (MQ=255)                                                                                 cTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAgt                                                >  1:513178/1‑140 (MQ=255)                                                                                    aaCATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGtgtg                                             >  1:34069/1‑140 (MQ=255)                                                                                     aCATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGtga                                            >  2:1058506/1‑140 (MQ=255)                                                                                       aTAGATACCTCCTTGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGtgatg                                          <  1:582997/140‑1 (MQ=255)                                                                                       aTAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGtgatg                                          <  2:411967/140‑1 (MQ=255)                                                                                       aTAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGtgatg                                          <  2:318347/140‑1 (MQ=255)                                                                                       aTAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGtgatg                                          <  1:89243/140‑1 (MQ=255)                                                                                       aTAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGtgatg                                          <  1:614783/140‑1 (MQ=255)                                                                                           aTACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGTGATGAACt                                      >  2:574306/1‑140 (MQ=255)                                                                                              cctcctCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGTGATGAACTTCa                                   >  1:853016/1‑140 (MQ=255)                                                                                              cctcctCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGTGATGAACTTCa                                   >  1:407667/1‑140 (MQ=255)                                                                                                tcctcGACGGCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGTGATGAACTTCaaa                                 >  1:376545/1‑140 (MQ=255)                                                                                                   tcGACGGCTGGCTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGAGGGTGACCATTTCTGATGCAGTTGTTCAAAAAAAACACCATGATGAAGTGTGATGAACTTCAAATc                               >  2:46008/1‑140 (MQ=255)                                                                                                         gCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGTGATGAACTTCAAATCAGCgtgt                        >  2:626571/1‑140 (MQ=255)                                                                                                         gCTGACTTTGTGTGCTCTCCTCTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGTGATGAACTTCAAATCAGCgtgt                        <  1:861492/140‑1 (MQ=255)                                                                                                                         ctcctcTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGTGATGAACTTCAAATCAGCGTGTTa                      <  2:156807/126‑1 (MQ=255)                                                                                                                         ctcctcTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGTGATGAACTTCAAATCAGCGTGTTa                      >  1:156807/1‑126 (MQ=255)                                                                                                                          tcctcTGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGTGATGAACTTCAAATCAGCGTGTTAGAGGTTAATTGCGaa       <  2:624194/140‑1 (MQ=255)                                                                                                                            ctctGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGTGATGAACTTCAAATCAGCGTGTTAGAGGTTAATTGCGAAAg     >  1:30712/1‑140 (MQ=255)                                                                                                                              ctGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGTGATGAACTTCAAATCAGCGTGTTAGAGGTTAATTGCGAAAggg   <  2:640243/140‑1 (MQ=255)                                                                                                                              ctGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGTGATGAACTTCAAATCAGCGTGTTAGAGGTTAATTGCGAAAggg   <  1:171298/140‑1 (MQ=255)                                                                                                                              ctGTGATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGTGATGAACTTCAAATCAGCGTGTTAGAGGTTAATTGCGAAAggg   <  2:126310/140‑1 (MQ=255)                                                                                                                               tgtgATAATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGTGATGAACTTCAAATCAGCGTGTTAGAGGTTAATTGCGAAAgggg  <  2:825609/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       TGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTTGTTC‑AAAAAAACACCATGATGAAGTGTGATGAACTTCAAATCAGCGTGTTAGAGGTTAATTGCGAAAGGGG  >  NC_000913/4424704‑4424969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑