Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,467,176	A→G	V24A (GTG→GCG)	treR ←	DNA‑binding transcriptional repressor TreR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,467,176	0	A	G	100.0%	264.5 / NA	73	V24A (GTG→GCG)	treR	DNA‑binding transcriptional repressor TreR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/41);  total (32/41)

ACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCAGCAATGGG  >  NC_000913/4467041‑4467312                                                                                                                                        |                                                                                                                                         aCAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCg                                                                                                                                      <  1:220807/140‑1 (MQ=255)        tgGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACt                                                                                                                               >  1:991235/1‑140 (MQ=255)        tgGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACt                                                                                                                               >  2:413368/1‑140 (MQ=255)         ggCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTg                                                                                                                              >  2:805289/1‑140 (MQ=255)           cGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTa                                                                                                                            >  1:591595/1‑140 (MQ=255)             aCCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCg                                                                                                                                      >  1:300514/1‑128 (MQ=255)             aCCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCg                                                                                                                                      <  2:300514/128‑1 (MQ=255)             aCCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCAGGGAAACTGTAGa                                                                                                                          >  1:911723/1‑140 (MQ=255)                 cTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTc                                                                                                                      >  1:213948/1‑140 (MQ=255)                   ttATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTccc                                                                                                                    <  2:1015507/140‑1 (MQ=255)                    tATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTcccc                                                                                                                   >  2:703265/1‑140 (MQ=255)                     aTCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCa                                                                                                                  <  2:423113/140‑1 (MQ=255)                     aTCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCa                                                                                                                  <  2:591595/140‑1 (MQ=255)                            tGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCt                                                                                                           >  2:478399/1‑140 (MQ=255)                            tGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCt                                                                                                           >  2:141437/1‑140 (MQ=255)                                cACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAg                                                                                                       <  1:860497/140‑1 (MQ=255)                                   gCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAgcgc                                                                                                    >  2:845960/1‑140 (MQ=255)                                    cATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAgcgcg                                                                                                   <  1:1044548/140‑1 (MQ=255)                                        gcgcgcgcAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGat                                                                                               >  1:801086/1‑140 (MQ=255)                                        gcgcgcgcAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGat                                                                                               >  2:111688/1‑140 (MQ=255)                                          gcgcgcAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGatat                                                                                             <  2:834102/140‑1 (MQ=255)                                             cgcAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAgcgcgc                                                                                                  <  1:742043/132‑1 (MQ=255)                                             cgcAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCtt                                                                                          >  2:144645/1‑140 (MQ=255)                                             cgcAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCAGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAgcgcgc                                                                                                  >  2:742043/1‑132 (MQ=255)                                                            ggAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGAt                                                                           >  2:704305/1‑140 (MQ=255)                                                            ggAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGAt                                                                           >  1:848268/1‑140 (MQ=255)                                                             gAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGAtt                                                                          <  1:667100/140‑1 (MQ=255)                                                                aaTCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTg                                                                       >  1:145437/1‑140 (MQ=255)                                                                  tCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCa                                                                     <  1:585462/140‑1 (MQ=255)                                                                    cATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATc                                                                   <  1:434103/140‑1 (MQ=255)                                                                    cATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATc                                                                   <  1:1043982/140‑1 (MQ=255)                                                                    cATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATc                                                                   <  2:848268/140‑1 (MQ=255)                                                                     aTGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATcc                                                                  <  2:902711/140‑1 (MQ=255)                                                                       gCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCtg                                                                <  1:845960/140‑1 (MQ=255)                                                                       gCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCtg                                                                <  1:495809/140‑1 (MQ=255)                                                                       gCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCtg                                                                <  1:738931/140‑1 (MQ=255)                                                                       gCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCtg                                                                <  1:111688/140‑1 (MQ=255)                                                                         tGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCtgtt                                                              <  2:237712/140‑1 (MQ=255)                                                                            ttCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTc                                                           <  2:846406/140‑1 (MQ=255)                                                                                 caCTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTa                                                      <  2:801086/140‑1 (MQ=255)                                                                                      cTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACgtg                                                 >  2:924739/1‑140 (MQ=255)                                                                                      cTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACgtg                                                 <  2:991235/140‑1 (MQ=255)                                                                                      cTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACgtg                                                 <  1:365388/140‑1 (MQ=255)                                                                                      cTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACgtg                                                 >  1:56770/1‑140 (MQ=255)                                                                                              cGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTaa                                         >  1:337788/1‑140 (MQ=255)                                                                                                cTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGtgttgt                                             <  2:736557/134‑1 (MQ=255)                                                                                                cTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGtgttgt                                             >  1:736557/1‑134 (MQ=255)                                                                                                cTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAatt                                       >  2:903079/1‑140 (MQ=255)                                                                                                cTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAatt                                       >  2:700808/1‑140 (MQ=255)                                                                                                 tCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAatta                                      >  1:401528/1‑140 (MQ=255)                                                                                                 tCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAatta                                      >  2:546567/1‑140 (MQ=255)                                                                                                   gcgGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAattatt                                    <  1:924739/140‑1 (MQ=255)                                                                                                    cgGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTAttt                                   <  2:401528/140‑1 (MQ=255)                                                                                                        tGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGc                               <  1:413368/140‑1 (MQ=255)                                                                                                        tGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGc                               <  2:56770/140‑1 (MQ=255)                                                                                                        tGAGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGc                               <  1:288997/140‑1 (MQ=255)                                                                                                          aGCTGGCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCt                             >  2:346057/1‑140 (MQ=255)                                                                                                             tGGCTCACGCCGCTTTCGTTATTCAGCGCCAGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAAc                          <  2:911723/140‑1 (MQ=255)                                                                                                               gCTCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGt                        >  2:1002722/1‑140 (MQ=255)                                                                                                                 tCACGCCGCTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTa                      <  1:700808/140‑1 (MQ=255)                                                                                                                    cgccgcTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTAccc                   >  1:692674/1‑140 (MQ=255)                                                                                                                    cgccgcTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTAccc                   <  1:630404/140‑1 (MQ=255)                                                                                                                    cgccgcTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTAccc                   >  1:988247/1‑140 (MQ=255)                                                                                                                      ccgcTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGt                 <  2:337788/140‑1 (MQ=255)                                                                                                                      ccgcTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGt                 <  1:927908/140‑1 (MQ=255)                                                                                                                         cTTTCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCa              >  1:41049/1‑140 (MQ=255)                                                                                                                           ttCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGt                 <  1:972468/135‑1 (MQ=255)                                                                                                                           ttCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGt                 >  2:972468/1‑135 (MQ=255)                                                                                                                           ttCGTTATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCAtt            <  1:550150/140‑1 (MQ=255)                                                                                                                               ttATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCAGc        <  1:805289/140‑1 (MQ=255)                                                                                                                                tATTCAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCAGCa       <  2:145437/140‑1 (MQ=255)                                                                                                                                    cAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCa          >  2:766848/1‑133 (MQ=255)                                                                                                                                    cAGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCa          <  1:766848/133‑1 (MQ=255)                                                                                                                                     aGCGCCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCAGCAATggg  <  1:903079/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         ACAATGATGGCGACCACTTTATCGCTTTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCCGGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCAGCAATGGG  >  NC_000913/4467041‑4467312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑