Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,480,758	T→C	G343G (GGT→GGC)	yjgN →	conserved inner membrane protein YjgN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,480,758	0	T	C	100.0%	268.4 / NA	75	G343G (GGT→GGC)	yjgN	conserved inner membrane protein YjgN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/38);  total (37/38)

ACGGTGTCTTTGCGAAACCATTTTATGAGCAACCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGTCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATT  >  NC_000913/4480621‑4480890                                                                                                                                          |                                                                                                                                     aCGGTGTCTTTGCGAAACCATTTTATGAGCAACCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCt                                                                                                                                    <  2:865755/140‑1 (MQ=255)       tCTTTGCGAAACCATTTTATGAGCAACCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCt                                                                                                                                    >  2:275918/1‑134 (MQ=255)       tCTTTGCGAAACCATTTTATGAGCAACCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCt                                                                                                                                    <  1:352587/134‑1 (MQ=255)       tCTTTGCGAAACCATTTTATGAGCAACCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCt                                                                                                                                    >  2:352587/1‑134 (MQ=255)       tCTTTGCGAAACCATTTTATGAGCAACCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTa                                                                                                                              >  2:956671/1‑140 (MQ=255)       tCTTTGCGAAACCATTTTATGAGCAACCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTa                                                                                                                              >  1:825656/1‑140 (MQ=255)         tttGCGAAACCATTTTATGAGCAACCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATc                                                                                                                            <  2:1054261/140‑1 (MQ=255)         tttGCGAAACCATTTTATGAGCAACCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATc                                                                                                                            <  2:174504/140‑1 (MQ=255)         tttGCGAAACCATTTTATGAGCAACCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATc                                                                                                                            <  2:321329/140‑1 (MQ=255)           tGCGAAACCATTTTATGAGCATCCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCa                                                                                                                          <  2:987321/140‑1 (MQ=255)                aaCCATTTTATGAGCAACCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCActgct                                                                                                                     <  1:1048348/140‑1 (MQ=255)                aaCCATTTTATGAGCAACCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGACTACGGGCGGCCTGGCTTATCCActgct                                                                                                                     <  2:47416/140‑1 (MQ=255)                         aTGAGCAACCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATg                                                                                                            <  1:270900/140‑1 (MQ=255)                           gAGCAACCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGga                                                                                                          <  2:123798/140‑1 (MQ=255)                           gAGCAACCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGga                                                                                                          <  1:164513/140‑1 (MQ=255)                               aaCCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGga                                                                                                          >  2:780073/1‑136 (MQ=255)                               aaCCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGga                                                                                                          <  1:780073/136‑1 (MQ=255)                                aCCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGAtt                                                                                                     >  2:282584/1‑140 (MQ=255)                                           aaTGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTggcaggc                                                                                          >  2:163289/1‑140 (MQ=255)                                           aaTGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTggcagg                                                                                           <  2:951033/139‑1 (MQ=255)                                           aaTGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTggcagg                                                                                           >  1:951033/1‑139 (MQ=255)                                               atgGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTggca                                                                                             <  1:612812/133‑1 (MQ=255)                                               atgGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTggca                                                                                             >  2:612812/1‑133 (MQ=255)                                               atgGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCaaaa                                                                                      >  2:419261/1‑140 (MQ=255)                                               atgGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCaaaa                                                                                      >  2:318569/1‑140 (MQ=255)                                               atgGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCaaaa                                                                                      >  2:469216/1‑140 (MQ=255)                                               atgGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCaaaa                                                                                      >  1:252914/1‑140 (MQ=255)                                               atgGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCaaaa                                                                                      >  1:151671/1‑140 (MQ=255)                                                   gCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATa                                                                                  <  1:666860/140‑1 (MQ=255)                                                   gCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATa                                                                                  >  2:666860/1‑140 (MQ=255)                                                      tATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGt                                                                               <  1:282584/140‑1 (MQ=255)                                                      tATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGt                                                                               <  2:1050914/140‑1 (MQ=255)                                                       aTTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTa                                                                              >  2:578561/1‑140 (MQ=255)                                                             tttCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCt                                                                        >  2:823991/1‑140 (MQ=255)                                                                cGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTggg                                                                     <  2:1054221/140‑1 (MQ=255)                                                                   ttAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGa                                                                  <  1:407972/140‑1 (MQ=255)                                                                          tAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGAt                                                           <  2:1068756/140‑1 (MQ=255)                                                                          tAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGAt                                                           <  1:163289/140‑1 (MQ=255)                                                                          tAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGAt                                                           <  1:956671/140‑1 (MQ=255)                                                                             cGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGAc                                                        <  2:285242/140‑1 (MQ=255)                                                                              gTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGAcc                                                       <  2:373607/140‑1 (MQ=255)                                                                              gTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGAcc                                                       <  2:63742/140‑1 (MQ=255)                                                                              gTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGAcc                                                       <  2:322336/140‑1 (MQ=255)                                                                              gTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGAcc                                                       <  1:471055/140‑1 (MQ=255)                                                                              gTACCACGGTATGCTTTATCGCATGTCTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGAcc                                                       <  1:758956/140‑1 (MQ=255)                                                                                 ccACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGAccttc                                                    >  1:502218/1‑140 (MQ=255)                                                                                 ccACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGAccttc                                                    >  1:663661/1‑140 (MQ=255)                                                                                 ccACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTACTTGCTGGGCGATTTGGATGAccttc                                                    >  2:615069/1‑140 (MQ=255)                                                                                  cACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGAccttcc                                                   >  2:364775/1‑140 (MQ=255)                                                                                   aCGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGAccttcct                                                  <  1:419261/140‑1 (MQ=255)                                                                                       tATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTaa                                              >  2:730705/1‑140 (MQ=255)                                                                                          gCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCa                                           <  2:825656/140‑1 (MQ=255)                                                                                               aTCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATaaa                                      >  1:561390/1‑140 (MQ=255)                                                                                               aTCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATaaa                                      <  2:1055067/140‑1 (MQ=255)                                                                                                   cATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAaagaag                                  >  1:473053/1‑140 (MQ=255)                                                                                                     tgtgtgCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAgaagaa                                >  2:80953/1‑140 (MQ=255)                                                                                                     tgtgtgCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAgaagaa                                >  1:126141/1‑140 (MQ=255)                                                                                                     tgtgtgCGTTGGTGGTGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAgaagaa                                <  2:561390/140‑1 (MQ=255)                                                                                                               ggtggtGATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATa                      >  2:913724/1‑140 (MQ=255)                                                                                                                 tggtgATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATaaa                    <  1:730705/140‑1 (MQ=255)                                                                                                                 tggtgATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATaaa                    <  1:80953/140‑1 (MQ=255)                                                                                                                 tggtgATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATaaa                    >  1:451680/1‑140 (MQ=255)                                                                                                                   gtgATATCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAgg                  >  2:560114/1‑140 (MQ=255)                                                                                                                       tatCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAGGcttc              <  1:560114/140‑1 (MQ=255)                                                                                                                       tatCCGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAGGcttc              >  2:190383/1‑140 (MQ=255)                                                                                                                          ccGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGaacaac                            >  1:716221/1‑123 (MQ=255)                                                                                                                          ccGGGATTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGaacaac                            <  2:716221/123‑1 (MQ=255)                                                                                                                               aTTACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAg      <  1:823991/140‑1 (MQ=255)                                                                                                                                 tACGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTa    >  2:1064906/1‑140 (MQ=255)                                                                                                                                  aCGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTAt   <  1:913724/140‑1 (MQ=255)                                                                                                                                   cGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTAtt  >  2:166225/1‑140 (MQ=255)                                                                                                                                   cGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTAtt  >  2:895452/1‑140 (MQ=255)                                                                                                                                   cGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTAtt  >  2:231506/1‑140 (MQ=255)                                                                                                                                   cGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTAtt  >  1:964205/1‑140 (MQ=255)                                                                                                                                   cGGGCGGCCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCCTCTTAGCCAGTAtt  >  1:364778/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     ACGGTGTCTTTGCGAAACCATTTTATGAGCAACCTGTCACTGAATGATGGGCGTATTCGTTTTCGCTTAACTTTAACGTACCACGGTATGCTTTATCGCATGTGTGCGTTGGTGGTGATATCCGGGATTACGGGCGGTCTGGCTTATCCACTGCTGAAAATATGGATGATTGACTGGCAGGCAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATT  >  NC_000913/4480621‑4480890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑