Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,543,321	A→G	G69G (GGA→GGG)	fimA →	type 1 fimbriae major subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,543,321	0	A	G	100.0%	312.8 / NA	88	G69G (GGA→GGG)	fimA	type 1 fimbriae major subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (51/37);  total (51/37)

CTGCCACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGAGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCA  >  NC_000913/4543185‑4543449                                                                                                                                         |                                                                                                                                 cTGCCACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCa                                                                                                                               >  2:892142/1‑140 (MQ=255)      acgacgGTTAATGGTGGGACCGTTCACTTTAAAGGGAAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAg                                                                                                                          <  2:442851/140‑1 (MQ=255)              tAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTctgctg                                                                                                                  <  1:33170/140‑1 (MQ=255)              tAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTctgctg                                                                                                                  >  1:464090/1‑140 (MQ=255)                 tggtggGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCg                                                                                                               >  1:843725/1‑140 (MQ=255)                    tggGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCGGGCGCAGGAAGGGGCAACCAGTTCTGCTGTCGGtt                                                                                                            >  2:62256/1‑140 (MQ=255)                      ggACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGtttt                                                                                                          >  2:322399/1‑140 (MQ=255)                      ggACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGtttt                                                                                                          >  1:1073724/1‑140 (MQ=255)                      ggACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGtttt                                                                                                          >  1:488589/1‑140 (MQ=255)                         ccGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAAc                                                                                                       >  1:647229/1‑140 (MQ=255)                         ccGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAAc                                                                                                       <  2:422076/140‑1 (MQ=255)                           gTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACAt                                                                                                     >  1:18546/1‑140 (MQ=255)                            ttCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTCGGTCAGGGTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACAtt                                                                                                    >  2:948528/1‑140 (MQ=255)                            ttCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTc                                                                                                                >  2:837256/1‑128 (MQ=255)                            ttCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTc                                                                                                                <  1:837256/128‑1 (MQ=255)                            ttCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACAtt                                                                                                    >  2:128599/1‑140 (MQ=255)                            ttCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACAtt                                                                                                    <  1:265996/140‑1 (MQ=255)                             tCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTc                                                                                                   >  2:488106/1‑140 (MQ=255)                              cACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTc                                                                                                                >  1:777394/1‑126 (MQ=255)                              cACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTc                                                                                                                <  2:777394/126‑1 (MQ=255)                              cACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCgg                                                                                                              <  2:704635/128‑1 (MQ=255)                              cACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCgg                                                                                                              >  1:704635/1‑128 (MQ=255)                                   tAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGa                                                                                             >  1:158047/1‑140 (MQ=255)                                      aGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATg                                                                                          >  2:4128/1‑140 (MQ=255)                                      aGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATg                                                                                          >  1:843801/1‑140 (MQ=255)                                           aaGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGc                                                                                     >  2:349675/1‑140 (MQ=255)                                             gttgttAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGa                                                                                   <  2:158047/140‑1 (MQ=255)                                                  tAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCa                                                                              <  1:488106/140‑1 (MQ=255)                                                       ccgcTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGtt                                                                         >  2:1040496/1‑140 (MQ=255)                                                        cgcTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTg                                                                        <  1:128599/140‑1 (MQ=255)                                                        cgcTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTg                                                                        <  1:1040496/140‑1 (MQ=255)                                                        cgcTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTCTCGGTTTTAACATTCAGCTAAATGATTGCGATACCAATGTTg                                                                        >  2:1006123/1‑140 (MQ=255)                                                          cTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCa                                                                      >  1:353768/1‑140 (MQ=255)                                                                       tGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTg                                                         >  1:897035/1‑140 (MQ=255)                                                                         cAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGtt                                                       >  2:753950/1‑140 (MQ=255)                                                                          aGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTg                                                      <  1:4128/140‑1 (MQ=255)                                                                          aGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTg                                                      <  2:347517/140‑1 (MQ=255)                                                                           ggCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCg                                                            >  1:1084771/1‑133 (MQ=255)                                                                           ggCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCg                                                            >  1:653341/1‑133 (MQ=255)                                                                           ggCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCg                                                            <  2:1084771/133‑1 (MQ=255)                                                                           ggCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCg                                                            <  2:653341/133‑1 (MQ=255)                                                                           ggCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGc                                                     >  1:389689/1‑140 (MQ=255)                                                                           ggCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGc                                                     >  1:593749/1‑140 (MQ=255)                                                                             ctctGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTg                                                         >  2:116985/1‑134 (MQ=255)                                                                             ctctGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTg                                                         <  1:116985/134‑1 (MQ=255)                                                                                tGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCtttt                                                <  2:18546/140‑1 (MQ=255)                                                                                 gTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCttttt                                               <  2:593749/140‑1 (MQ=255)                                                                                  ttGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTa                                              >  2:932466/1‑140 (MQ=255)                                                                                      tCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTa                                          >  2:1056614/1‑140 (MQ=255)                                                                                       cAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTAc                                         <  2:488589/140‑1 (MQ=255)                                                                                       cAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTAc                                         >  2:77870/1‑140 (MQ=255)                                                                                       cAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTAc                                         <  2:843801/140‑1 (MQ=255)                                                                                          aCCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGc                                      >  2:100216/1‑140 (MQ=255)                                                                                          aCCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGc                                      >  1:108560/1‑140 (MQ=255)                                                                                            cGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGa                                    >  2:971926/1‑140 (MQ=255)                                                                                             gTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGAt                                   <  1:753950/140‑1 (MQ=255)                                                                                               tCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTg                                 >  1:228091/1‑140 (MQ=255)                                                                                                 aGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGAt                               <  1:684413/140‑1 (MQ=255)                                                                                                 aGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGAt                               <  1:451545/140‑1 (MQ=255)                                                                                                  gTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATg                              <  2:897035/140‑1 (MQ=255)                                                                                                  gTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATg                              <  2:843725/140‑1 (MQ=255)                                                                                                  gTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATg                              <  2:108560/140‑1 (MQ=255)                                                                                                  gTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATg                              <  1:892142/140‑1 (MQ=255)                                                                                                  gTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATg                              <  1:932466/140‑1 (MQ=255)                                                                                                  gTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATg                              <  1:971926/140‑1 (MQ=255)                                                                                                      ggACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGAt                               <  1:1084903/135‑1 (MQ=255)                                                                                                      ggACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGAt                               >  2:1084903/1‑135 (MQ=255)                                                                                                      ggACAGGTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCggg                          <  1:1056614/140‑1 (MQ=255)                                                                                                            gTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGAt                                   >  1:1033497/1‑125 (MQ=255)                                                                                                            gTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGAt                                   <  2:1033497/125‑1 (MQ=255)                                                                                                            gTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATAc                    >  1:200243/1‑140 (MQ=255)                                                                                                            gTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATAc                    >  2:710300/1‑140 (MQ=255)                                                                                                            gTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATAc                    >  2:525103/1‑140 (MQ=255)                                                                                                            gTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATAc                    >  2:1076748/1‑140 (MQ=255)                                                                                                            gTTCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTAGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATAc                    >  2:244045/1‑140 (MQ=255)                                                                                                             ttCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATAcc                   >  2:300080/1‑140 (MQ=255)                                                                                                             ttCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATAcc                   >  1:623391/1‑140 (MQ=255)                                                                                                             ttCGTACCGCATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATAcc                   >  2:26311/1‑140 (MQ=255)                                                                                                                      cATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGt                         >  1:289174/1‑125 (MQ=255)                                                                                                                      cATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGt                         <  2:289174/125‑1 (MQ=255)                                                                                                                      cATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTg          >  2:183897/1‑140 (MQ=255)                                                                                                                      cATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAACGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCaa                 <  2:65511/133‑1 (MQ=255)                                                                                                                      cATCGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAACGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCaa                 >  1:65511/1‑133 (MQ=255)                                                                                                                         cGCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGct       >  1:819122/1‑140 (MQ=255)                                                                                                                          gCTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGctc      >  2:6422/1‑140 (MQ=255)                                                                                                                           cTGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGctct     >  2:210346/1‑140 (MQ=255)                                                                                                                            tGGCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTg    <  1:300080/140‑1 (MQ=255)                                                                                                                              gCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCa  <  1:210346/140‑1 (MQ=255)                                                                                                                              gCACAGGAAGGGGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCa  <  1:525103/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 CTGCCACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCACAGGAAGGAGCAACCAGTTCTGCTGTCGGTTTTAACATTCAGCTGAATGATTGCGATACCAATGTTGCATCTAAAGCCGCTGTTGCCTTTTTAGGTACGGCGATTGATGCGGGTCATACCAACGTTCTGGCTCTGCA  >  NC_000913/4543185‑4543449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑