Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 413,307 T→C D816G (GAC→GGC)  sbcC ← ATP dependent, structure specific DNA nuclease ‑ SbcC subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913413,3070TC100.0% 189.1 / NA 53D816G (GAC→GGC) sbcCATP dependent, structure specific DNA nuclease ‑ SbcC subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/23);  total (30/23)

GCTGACGGTTATCTGCATCCTGCTTCAGCTGCTGGCGAATCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGTCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCG  >  NC_000913/413170‑413442
                                                                                                                                         |                                                                                                                                       
gCTGACGGTTATCTGCATCCTGCTTCAGCTGCTGGCGAATCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCa                                                                                                                                       >  2:843818/1‑140 (MQ=255)
   gACGGTTATCTGCATCCTGCTTCAGCTGCTGGCGAATCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGt                                                                                                                                    <  1:382377/140‑1 (MQ=255)
     cGGTTATCTGCATCCTGCTTCAGCTGCTGGCGAATCTCACCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCg                                                                                                                                  <  2:1001216/140‑1 (MQ=255)
       gTTATCTGCATCCTGCTTCAGCTGCTGGCGAATCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGgt                                                                                                                                <  1:277302/140‑1 (MQ=255)
       gTTATCTGCATCCTGCTTCAGCTGCTCGCGAATCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGgt                                                                                                                                <  1:758145/140‑1 (MQ=255)
        ttATCTGCATCCTGCTTCAGCTGCTGGCGAATCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGgtg                                                                                                                               <  1:293628/140‑1 (MQ=255)
           tCTGCATCCTGCTTCAGCTGCTGGCGAATCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCg                                                                                                                                  <  2:693699/134‑1 (MQ=255)
           tCTGCATCCTGCTTCAGCTGCTGGCGAATCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCg                                                                                                                                  >  1:693699/1‑134 (MQ=255)
                aTCCTGCTTCAGCTGCTGGCGAATCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGAt                                                                                                                       <  2:86411/140‑1 (MQ=255)
                  ccTGCTTCAGCTGCTGGCGAATCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGc                                                                                                                     >  1:1080545/1‑140 (MQ=255)
                    tGCTTCAGCTGCTGGCGAATCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCtg                                                                                                                   >  2:785019/1‑140 (MQ=255)
                    tGCTTCAGCTGCTGGCGAATCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCtg                                                                                                                   >  1:720064/1‑140 (MQ=255)
                      cTTCAGCTGCTGGCGAATCTCGCCTTGACTCATGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCtgtg                                                                                                                 >  1:1046250/1‑140 (MQ=255)
                            ctgctgGCGAATCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCg                                                                                                           >  1:81016/1‑140 (MQ=255)
                            ctgctgGCGAATCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCg                                                                                                           >  2:637690/1‑140 (MQ=255)
                                tgGCGAATCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTc                                                                                                       <  1:801327/140‑1 (MQ=255)
                                 ggCGAATCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTct                                                                                                      >  1:956856/1‑140 (MQ=255)
                                     aaTCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCg                                                                                                           >  2:604361/1‑131 (MQ=255)
                                     aaTCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCg                                                                                                           <  1:604361/131‑1 (MQ=255)
                                     aaTCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTctgctg                                                                                                  <  2:956856/140‑1 (MQ=255)
                                                gacaggtggtgTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGAcc                                                                                       <  1:465193/135‑1 (MQ=255)
                                                gACTCGTGGTGTTTTCACGCATCTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGAcc                                                                                       >  2:768672/1‑140 (MQ=255)
                                                gACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGAcc                                                                                       >  1:970144/1‑140 (MQ=255)
                                                     gtggtgTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGt                                                                                  >  1:995579/1‑140 (MQ=255)
                                                          gtTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTgcg                                                                             <  1:468031/140‑1 (MQ=255)
                                                           ttttCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTgcgc                                                                            >  1:11338/1‑140 (MQ=255)
                                                                     aCTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGgcgc                                                                  >  1:417142/1‑140 (MQ=255)
                                                                     aCTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGgcgc                                                                  >  1:159609/1‑140 (MQ=255)
                                                                             tGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTc                                                          <  2:417142/140‑1 (MQ=255)
                                                                                   tGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGAtt                                                    <  1:461947/140‑1 (MQ=255)
                                                                                   tGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGAtt                                                    >  2:116098/1‑140 (MQ=255)
                                                                                        tAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCt                                               <  2:655332/140‑1 (MQ=255)
                                                                                           ctctTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGa                                            <  2:696018/140‑1 (MQ=255)
                                                                                           ctctTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGa                                            <  1:785019/140‑1 (MQ=255)
                                                                                            tctTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAg                                           <  2:1080545/140‑1 (MQ=255)
                                                                                                    aaTCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTcc                                    >  2:186854/1‑139 (MQ=255)
                                                                                                    aaTCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTcc                                    <  1:186854/139‑1 (MQ=255)
                                                                                                    aaTCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCa                                   >  2:1032550/1‑140 (MQ=255)
                                                                                                     aTCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAg                                  <  1:116098/140‑1 (MQ=255)
                                                                                                          cTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCt                                <  2:182700/137‑1 (MQ=255)
                                                                                                          cTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCt                                >  1:182700/1‑137 (MQ=255)
                                                                                                          cTCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCg                             >  2:458830/1‑140 (MQ=255)
                                                                                                           tCCACCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGt                            >  1:147879/1‑140 (MQ=255)
                                                                                                           ccccccGTTACAGTGAGAGCCAAACCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTTGGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGt                            <  2:716359/136‑1 (MQ=255)
                                                                                                              aCCGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAg                         >  1:1075037/1‑140 (MQ=255)
                                                                                                               ccGTCACAGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAgt                        >  1:130238/1‑140 (MQ=255)
                                                                                                                    acaGTGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGtgtttg                   >  2:534009/1‑140 (MQ=255)
                                                                                                                        tGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCa               >  1:193625/1‑140 (MQ=255)
                                                                                                                        tGAGAGCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCa               <  1:1003630/140‑1 (MQ=255)
                                                                                                                            agCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCa           >  1:429891/1‑140 (MQ=255)
                                                                                                                            agCCAACCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCa           >  2:845447/1‑140 (MQ=255)
                                                                                                                                aaCCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAg       <  2:720064/140‑1 (MQ=255)
                                                                                                                                aaCCCGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAg       >  1:462396/1‑140 (MQ=255)
                                                                                                                                  cccGTCGCCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAgcg     >  1:359524/1‑140 (MQ=255)
                                                                                                                                     gtcgccAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGcgccg  <  1:1032550/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GCTGACGGTTATCTGCATCCTGCTTCAGCTGCTGGCGAATCTCGCCTTGACTCGTGGTGTTTTCACGCAACTTTTGGTGAGTTTGCGCTAACTCTTGCTGAATCTGCTCCACCGTCACAGTGAGAGCCAACCCGTCGTCAGGTCGGTGTTGTTGATGCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCG  >  NC_000913/413170‑413442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑