Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	437,593	A→G	S181S (AGT→AGC)	yajO ←	1‑deoxyxylulose‑5‑phosphate synthase YajO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	437,593	0	A	G	100.0%	183.5 / NA	51	S181S (AGT→AGC)	yajO	1‑deoxyxylulose‑5‑phosphate synthase YajO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/20);  total (31/20)

TCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCTTTCCCGGCTTTTACCACGTCGTTGAGGGCTTCCAGCGTCTCT  >  NC_000913/437464‑437728                                                                                                                                  |                                                                                                                                        tcCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACaaa                                                                                                                                 <  2:654949/138‑1 (MQ=255) tcCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACaaa                                                                                                                                 >  1:654949/1‑138 (MQ=255)               tCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGc                                                                                                                 <  1:447984/140‑1 (MQ=255)                        cccTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTgg                                                                                                        >  1:372813/1‑140 (MQ=255)                             gCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTc                                                                                                   >  1:925913/1‑140 (MQ=255)                              ccAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTcc                                                                                                  <  2:618573/140‑1 (MQ=255)                                   gggCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGc                                                                                             <  1:559544/140‑1 (MQ=255)                                      cTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGa                                                                                                       <  1:99879/127‑1 (MQ=255)                                      cTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGa                                                                                                       >  2:99879/1‑127 (MQ=255)                                       tCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGa                                                                                                       >  2:697754/1‑126 (MQ=255)                                       tCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGa                                                                                                       <  1:697754/126‑1 (MQ=255)                                       tCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTg                                                                                              <  2:1041038/135‑1 (MQ=255)                                       tCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTg                                                                                              >  1:1041038/1‑135 (MQ=255)                                           tGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCaa                                                                                     >  2:152148/1‑140 (MQ=255)                                                  aCCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGa                                                                              >  2:980444/1‑140 (MQ=255)                                                      ccACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCg                                                                          <  1:152148/140‑1 (MQ=255)                                                      ccACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCg                                                                          <  2:407608/140‑1 (MQ=255)                                                        aCGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTg                                                                        >  2:655532/1‑140 (MQ=255)                                                                     aaCACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGAcgcg                                                           <  1:655532/140‑1 (MQ=255)                                                                      acacAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAg                                                                            <  2:777820/122‑1 (MQ=255)                                                                      acacAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAg                                                                            >  1:777820/1‑122 (MQ=255)                                                                          aGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGat                                                      >  1:167507/1‑140 (MQ=255)                                                                          aGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGat                                                      >  2:303418/1‑140 (MQ=255)                                                                            tGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGatat                                                    >  2:14039/1‑140 (MQ=255)                                                                             ggTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGAcgcg                                                           <  2:38481/132‑1 (MQ=255)                                                                             ggTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGAcgcg                                                           >  1:38481/1‑132 (MQ=255)                                                                                 gCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAAcgc                                               >  1:650153/1‑140 (MQ=255)                                                                                  cATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAAcgcg                                              >  2:618018/1‑140 (MQ=255)                                                                                    tctcGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGGTATAACGCGCt                                            >  1:525369/1‑140 (MQ=255)                                                                                    tctcGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCt                                            >  1:801673/1‑140 (MQ=255)                                                                                      tcGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCttt                                          >  2:306641/1‑140 (MQ=255)                                                                                      tcGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCttt                                          >  2:381890/1‑140 (MQ=255)                                                                                       cgcgCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCTTTc                                         <  1:306641/140‑1 (MQ=255)                                                                                        gcgcTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCACTTTcc                                        >  1:739037/1‑140 (MQ=255)                                                                                             cttcttCACGATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGCGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCTTTCCCGGCt                                   >  2:733639/1‑140 (MQ=255)                                                                                                      gATAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCTTTCCCGGCTTTTACCAcg                          <  1:14039/140‑1 (MQ=255)                                                                                                       aTAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCTTTCCCGGCTTTTACCAcgt                         <  1:618018/140‑1 (MQ=255)                                                                                                        tAAATCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCTTTCCCGGCTTTTACCAcgtc                        >  1:314265/1‑140 (MQ=255)                                                                                                          aaTCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGGTATAACGCGCTTTCCCGGCTTTTACCAcgtcgt                      <  2:525369/140‑1 (MQ=255)                                                                                                          aaTCAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCTTTCCCGGCTTTTACCAcgtcgt                      >  1:59519/1‑140 (MQ=255)                                                                                                             cAGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTT‑GAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCTTTCCCGGCTTTTACCACGTCGTTGAg                  <  2:644622/140‑1 (MQ=255)                                                                                                              aGATTGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCTTTCCCGGCTTTTACCACGTCGTTGAg                  <  2:739037/140‑1 (MQ=255)                                                                                                                  tGTAGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCTTTCCCGGCTTTTACCACGTCGTTGAGGGCt              >  2:792458/1‑140 (MQ=255)                                                                                                                     aGTGATCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCTTTCCCGGCTTTTACCACGTCGTTGAGGGCTTcc           >  1:93378/1‑140 (MQ=255)                                                                                                                         aTCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCTTTCCCGGCTTTTACCACGTCGTTGAGGGCTTCCAGCg       <  1:699293/140‑1 (MQ=255)                                                                                                                         aTCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCTTTCCCGGCTTTTACCACGTCGTTGAGGGCTTCCAGCg       >  2:699293/1‑140 (MQ=255)                                                                                                                          tCCTGCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCTTTCCCGGCTTTTACCACGTCGTTGAGGGCTTCCAGCGt      >  1:387394/1‑140 (MQ=255)                                                                                                                              gCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCTTTCCCGGCTTTTACCACGTCGTTGAGGGCTTCCAGCGTctct  >  2:1081537/1‑140 (MQ=255)                                                                                                                              gCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCTTTCCCGGCTTTTACCACGTCGTTGAGGGCTTCCAGCGTctct  >  2:449760/1‑140 (MQ=255)                                                                                                                              gCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCTTTCCCGGCTTTTACCACGTCGTTGAGGGCTTCCAGCGTctct  >  1:805752/1‑140 (MQ=255)                                                                                                                              gCATGCTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCTTTCCCGGCTTTTACCACGTCGTTGAGGGCTTCCAGCGTctct  <  2:372813/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                        TCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACGCGCTTTCCCGGCTTTTACCACGTCGTTGAGGGCTTCCAGCGTCTCT  >  NC_000913/437464‑437728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑