Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	9,074	C→T	S279S (TCC→TCT)	talB →	transaldolase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	9,074	0	C	T	100.0%	258.5 / NA	72	S279S (TCC→TCT)	talB	transaldolase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/44);  total (28/44)

GGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGG  >  NC_000913/8945‑9210                                                                                                                                  |                                                                                                                                         ggCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCtgt                                                                                                                                <  1:317031/140‑1 (MQ=255)  gCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCtgtg                                                                                                                               >  2:76821/1‑140 (MQ=255)      gCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGgcag                                                                                                                           >  1:799501/1‑140 (MQ=255)      gCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGgcag                                                                                                                           <  1:869249/140‑1 (MQ=255)        tGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGgcagca                                                                                                                         <  1:595629/140‑1 (MQ=255)         gCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGcac                                                                                                                        <  2:952669/140‑1 (MQ=255)            aCCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAAc                                                                                                                     <  1:589344/140‑1 (MQ=255)              cGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCa                                                                                                                   <  2:386964/140‑1 (MQ=255)              cGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCa                                                                                                                   <  1:886689/140‑1 (MQ=255)               gTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAg                                                                                                                  <  1:986883/140‑1 (MQ=255)               gTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAg                                                                                                                  <  2:727259/140‑1 (MQ=255)                tCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAgg                                                                                                                 >  2:552164/1‑140 (MQ=255)                tCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAgg                                                                                                                 >  1:1015035/1‑140 (MQ=255)                  tGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGAt                                                                                                               <  1:552164/140‑1 (MQ=255)                      cATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCaa                                                                                                           <  2:1036214/140‑1 (MQ=255)                      cATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCaa                                                                                                           <  1:879548/140‑1 (MQ=255)                         cGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCaa                                                                                                           <  2:447493/137‑1 (MQ=255)                         cGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCaa                                                                                                           >  1:447493/1‑137 (MQ=255)                         cGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATgg                                                                                                        <  2:993575/140‑1 (MQ=255)                                gCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGa                                                                                                 >  1:439779/1‑140 (MQ=255)                                   ctgctgAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGc                                                                                                       <  2:245395/131‑1 (MQ=255)                                   ctgctgAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGCCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGc                                                                                                       >  1:245395/1‑131 (MQ=255)                                    tgctgAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATaaa                                                                                             <  2:760208/140‑1 (MQ=255)                                     gctgAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAAc                                                                                            <  1:979052/140‑1 (MQ=255)                                      ctgAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGc                                                                                                       <  1:69753/128‑1 (MQ=255)                                      ctgAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGc                                                                                                       >  2:69753/1‑128 (MQ=255)                                      ctgAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACt                                                                                           >  2:172374/1‑140 (MQ=255)                                        gAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTgg                                                                                         >  2:524941/1‑140 (MQ=255)                                        gAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTgg                                                                                         <  1:947927/140‑1 (MQ=255)                                          aaGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCg                                                                                       <  2:1015035/140‑1 (MQ=255)                                            gagCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGa                                                                                     <  2:242716/140‑1 (MQ=255)                                            gagCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGa                                                                                     <  1:174527/140‑1 (MQ=255)                                            gagCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGa                                                                                     <  2:47327/140‑1 (MQ=255)                                              gCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGTGGAAg                                                                                   >  1:762246/1‑140 (MQ=255)                                              gCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAg                                                                                   <  1:524941/140‑1 (MQ=255)                                                tGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGt                                                                                 >  1:588183/1‑140 (MQ=255)                                                tGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGt                                                                                 <  2:588183/140‑1 (MQ=255)                                                tGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGt                                                                                 >  1:846378/1‑140 (MQ=255)                                                      agagCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGt                                                                           <  1:997069/140‑1 (MQ=255)                                                      agagCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGt                                                                           <  2:199830/140‑1 (MQ=255)                                                        agCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTaa                                                                         >  1:980850/1‑140 (MQ=255)                                                           gAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGtt                                                                      <  2:980850/140‑1 (MQ=255)                                                             aGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTg                                                                    >  1:552171/1‑140 (MQ=255)                                                                ggCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTa                                                                 <  1:857986/140‑1 (MQ=255)                                                                   tATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTg                                                              >  2:565844/1‑140 (MQ=255)                                                                    aTCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGa                                                             <  1:565844/140‑1 (MQ=255)                                                                           gTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGaa                                                      >  2:807695/1‑140 (MQ=255)                                                                                cTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACt                                                 <  2:552171/140‑1 (MQ=255)                                                                                   tCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGa                                              >  2:1074102/1‑140 (MQ=255)                                                                                   tCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGa                                              >  1:657471/1‑140 (MQ=255)                                                                                      tACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGaaaa                                           >  1:551567/1‑140 (MQ=255)                                                                                       acacCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGaaaaa                                          <  2:551567/140‑1 (MQ=255)                                                                                        cacCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGaaaaaa                                         >  2:1084060/1‑140 (MQ=255)                                                                                           cGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGa                                      >  1:983674/1‑140 (MQ=255)                                                                                             gCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATc                                    >  1:564330/1‑140 (MQ=255)                                                                                             gCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATc                                    >  1:789766/1‑140 (MQ=255)                                                                                                  gTGAAAGCGCGTCCGGCGCGTATCACTGAGTCTGAATTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGa                               <  2:294706/140‑1 (MQ=255)                                                                                                       aGCGCGTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATctgc                          >  1:824348/1‑140 (MQ=255)                                                                                                         cgcgTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATctgctg                        <  2:983674/140‑1 (MQ=255)                                                                                                         cgcgTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATctgctg                        <  2:657471/140‑1 (MQ=255)                                                                                                           cgTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTa                      <  2:41456/140‑1 (MQ=255)                                                                                                           cgTCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTa                      >  1:305377/1‑140 (MQ=255)                                                                                                             tCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAAt                    <  2:148819/140‑1 (MQ=255)                                                                                                             tCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAAt                    >  1:797449/1‑140 (MQ=255)                                                                                                             tCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAAt                    <  1:535413/140‑1 (MQ=255)                                                                                                             tCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAAt                    <  1:86075/140‑1 (MQ=255)                                                                                                             tCCGGCGCGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCCGCTGTAAt                    <  2:564330/140‑1 (MQ=255)                                                                                                                 gcgcGTATCACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCAtt                <  1:498370/140‑1 (MQ=255)                                                                                                                         cACTGAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGt        <  1:1074102/140‑1 (MQ=255)                                                                                                                             gAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGAcc    <  2:824348/140‑1 (MQ=255)                                                                                                                             gAGTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGAcc    <  2:799501/140‑1 (MQ=255)                                                                                                                               gTCTGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCgg  >  2:517556/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                         GGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGG  >  NC_000913/8945‑9210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑