Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	597,709	T→C	S77S (AGT→AGC)	cusB →	copper/silver export system membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	597,709	0	T	C	100.0%	171.2 / NA	48	S77S (AGT→AGC)	cusB	copper/silver export system membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/20);  total (28/20)

CCGCAGAACGTAAAATCTTATTCTGGTACGACCCAATGTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCA  >  NC_000913/597588‑597845                                                                                                                          |                                                                                                                                         ccGCAGAACGTAAAATCTTATTCTGGTACGACCCAATGTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTgcgc                                                                                                                        <  2:144741/140‑1 (MQ=255)                          gTACGACCCAATGTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGa                                                                                                              >  1:437545/1‑125 (MQ=255)                          gTACGACCCAATGTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGa                                                                                                              <  2:437545/125‑1 (MQ=255)                          gTACGACCCAATGTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATc                                                                                               >  1:342045/1‑140 (MQ=255)                                    aTGTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACg                                                                                                      >  1:1036805/1‑123 (MQ=255)                                    aTGTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACg                                                                                                      <  2:1036805/123‑1 (MQ=255)                                          cccAATACGCGGTTCGTTAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGc                                                                               >  2:989861/1‑140 (MQ=255)                                          cccAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGc                                                                               >  2:36400/1‑140 (MQ=255)                                          cccAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGc                                                                               >  2:188265/1‑140 (MQ=255)                                          cccAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGc                                                                               >  1:739583/1‑140 (MQ=255)                                             aaTACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTAc                                                                            >  2:217720/1‑140 (MQ=255)                                                      ttCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCAcgcg                                                                   >  2:518545/1‑140 (MQ=255)                                                      ttCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCAcgcg                                                                   >  2:138376/1‑140 (MQ=255)                                                        cGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCAcgcgcg                                                                 >  1:197131/1‑140 (MQ=255)                                                                     aaaTCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACttt                                                    >  2:952535/1‑140 (MQ=255)                                                                     aaaTCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACttt                                                    >  1:801363/1‑140 (MQ=255)                                                                       aTCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAGGAGAGCTCTGCGTCTGGCGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGAGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTg                                                  >  2:128470/1‑140 (MQ=255)                                                                       aTCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTg                                                  >  2:687190/1‑140 (MQ=255)                                                                        tCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGc                                                 <  2:342045/140‑1 (MQ=255)                                                                        tCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGc                                                 >  2:703435/1‑140 (MQ=255)                                                                             gTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACt                                                      <  2:2023/130‑1 (MQ=255)                                                                             gTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACt                                                      >  1:911040/1‑130 (MQ=255)                                                                             gTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACt                                                      >  1:2023/1‑130 (MQ=255)                                                                             gTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACt                                                      <  2:911040/130‑1 (MQ=255)                                                                                        tggatCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCg                                 <  1:952535/140‑1 (MQ=255)                                                                                        tggatCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCg                                 >  2:748177/1‑140 (MQ=255)                                                                                        tggatCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCg                                 >  1:196984/1‑140 (MQ=255)                                                                                        tggatCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCg                                 >  2:255741/1‑140 (MQ=255)                                                                                         ggatCTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGa                                >  2:121010/1‑140 (MQ=255)                                                                                             cTGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGt                            >  1:512071/1‑140 (MQ=255)                                                                                              tGGTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTc                           <  1:217720/140‑1 (MQ=255)                                                                                               ggTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCa                          >  2:902808/1‑140 (MQ=255)                                                                                               ggTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCa                          >  1:913559/1‑140 (MQ=255)                                                                                                gTGCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAg                         >  1:785670/1‑140 (MQ=255)                                                                                                  gCCGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGtt                       <  1:188265/140‑1 (MQ=255)                                                                                                   ccGAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTa                      <  1:138376/140‑1 (MQ=255)                                                                                                     gAAATATGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACa                    <  2:512071/140‑1 (MQ=255)                                                                                                           tGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGa                <  1:80000/138‑1 (MQ=255)                                                                                                           tGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGa                >  2:80000/1‑138 (MQ=255)                                                                                                           tGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGt              <  1:518545/140‑1 (MQ=255)                                                                                                           tGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGt              <  1:36400/140‑1 (MQ=255)                                                                                                           tGCCGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGt              >  2:659793/1‑140 (MQ=255)                                                                                                             ccGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTAt            <  1:989861/140‑1 (MQ=255)                                                                                                             ccGATGAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTAt            <  1:121010/140‑1 (MQ=255)                                                                                                                  gAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTa       >  1:885622/1‑140 (MQ=255)                                                                                                                  gAAGAGAGCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTa       <  1:687190/140‑1 (MQ=255)                                                                                                                       gagCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCa  <  2:801363/140‑1 (MQ=255)                                                                                                                       gagCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCa  <  1:659793/140‑1 (MQ=255)                                                                                                                       gagCTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCa  <  1:386392/140‑1 (MQ=255)                                                                                                                          |                                                                                                                                         CCGCAGAACGTAAAATCTTATTCTGGTACGACCCAATGTATCCCAATACGCGGTTCGATAAACCAGGTAAATCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCTGGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACGCGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAGTATGCCA  >  NC_000913/597588‑597845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑