Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	750,625	T→C	T515T (ACA→ACG)	ybgQ ←	putative fimbrial usher protein YbgQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	750,625	0	T	C	100.0%	303.1 / NA	85	T515T (ACA→ACG)	ybgQ	putative fimbrial usher protein YbgQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/46);  total (39/46)

ACCACCGTTACCGAAGGGGAGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCTGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCG  >  NC_000913/750490‑750760                                                                                                                                        |                                                                                                                                        accaccGTTACCGAAGGGGAGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGa                                                                                                                                     >  2:526488/1‑140 (MQ=255)  ccaccGTTACCGAAGGGGAGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGAt                                                                                                                                    >  1:144977/1‑140 (MQ=255)  ccaccGTTACCGAAGGGGAGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGAt                                                                                                                                    >  2:547596/1‑140 (MQ=255)        ttACCGAAGGGGAGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGc                                                                                                                              >  1:33665/1‑140 (MQ=255)        ttACCGAAGGGGAGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGc                                                                                                                              <  1:13802/140‑1 (MQ=255)        ttACCGAAGGGGAGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGc                                                                                                                              <  1:831777/140‑1 (MQ=255)        ttACCGAAGGGGAGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGc                                                                                                                              <  2:797252/140‑1 (MQ=255)        ttACCGAAGGGGAGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGc                                                                                                                              <  1:974195/140‑1 (MQ=255)            cGAAGGGGAGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTc                                                                                                                          <  2:638601/140‑1 (MQ=255)               aGGGGAGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTg                                                                                                                       >  2:878038/1‑140 (MQ=255)                ggggAGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGtt                                                                                                                                 <  2:186329/129‑1 (MQ=255)                ggggAGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGtt                                                                                                                                 >  1:186329/1‑129 (MQ=255)                ggggAGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTgg                                                                                                                      >  2:218510/1‑140 (MQ=255)                ggggAGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTgg                                                                                                                      >  1:683072/1‑140 (MQ=255)                 gggAGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGa                                                                                                                     <  2:834677/140‑1 (MQ=255)                   gAGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAgg                                                                                                                   <  2:190396/140‑1 (MQ=255)                    aGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGc                                                                                                                              <  2:257756/128‑1 (MQ=255)                    aGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGc                                                                                                                              >  1:257756/1‑128 (MQ=255)                      cGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGt                                                                                                                >  2:726152/1‑140 (MQ=255)                      cGAAATCGACAGATAAATCTGGTTGTCGCGAACTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGt                                                                                                                >  2:177340/1‑140 (MQ=255)                            cGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCAt                                                                                                          <  2:144977/140‑1 (MQ=255)                            cGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCAt                                                                                                          <  2:565293/140‑1 (MQ=255)                             gACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCAtt                                                                                                         <  1:726152/140‑1 (MQ=255)                                aGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTcca                                                                                                      <  2:402126/140‑1 (MQ=255)                                aGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTcca                                                                                                      <  2:703278/140‑1 (MQ=255)                                 gATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTccac                                                                                                     <  1:878038/140‑1 (MQ=255)                                 gATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTccac                                                                                                     <  1:627099/140‑1 (MQ=255)                                  aTAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTccacc                                                                                                    <  2:432422/140‑1 (MQ=255)                                          ggTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGa                                                                                            <  1:301352/140‑1 (MQ=255)                                              gTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTa                                                                                        <  1:268361/140‑1 (MQ=255)                                                   gATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAgg                                                                                   <  1:370843/140‑1 (MQ=255)                                                         tATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAGTATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCg                                                                             >  2:750853/1‑140 (MQ=255)                                                               cGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGa                                                                       >  1:646598/1‑140 (MQ=255)                                                               cGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGa                                                                       >  2:1018064/1‑140 (MQ=255)                                                               cGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGa                                                                       >  1:162298/1‑140 (MQ=255)                                                                 tAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGAtt                                                                     <  1:633487/140‑1 (MQ=255)                                                                  aGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCAAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTg                                                                    <  1:796089/140‑1 (MQ=255)                                                                     gggTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGtt                                                                                 <  2:817272/124‑1 (MQ=255)                                                                     gggTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGtt                                                                                 >  1:817272/1‑124 (MQ=255)                                                                       gTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGtt                                                               >  1:326787/1‑140 (MQ=255)                                                                        tCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGttt                                                              >  1:244431/1‑140 (MQ=255)                                                                        tCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGttt                                                              >  1:879992/1‑140 (MQ=255)                                                                         cGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTa                                                             >  1:74244/1‑140 (MQ=255)                                                                           tATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGt                                                           >  2:129391/1‑140 (MQ=255)                                                                           tATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGt                                                           <  2:316088/140‑1 (MQ=255)                                                                           tATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGt                                                           >  2:991584/1‑140 (MQ=255)                                                                            aTTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTg                                                          <  2:326787/140‑1 (MQ=255)                                                                              tGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGt                                                           >  1:1076122/1‑137 (MQ=255)                                                                              tGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGt                                                           <  2:1076122/137‑1 (MQ=255)                                                                                aaTGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGAtt                                                                     >  1:248496/1‑125 (MQ=255)                                                                                aaTGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGAtt                                                                     <  2:248496/125‑1 (MQ=255)                                                                                 aTGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTa                                                     <  2:74244/140‑1 (MQ=255)                                                                                  tGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTaa                                                    >  1:937451/1‑140 (MQ=255)                                                                                  tGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTaa                                                    >  2:223931/1‑140 (MQ=255)                                                                                  tGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAAGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTaa                                                    >  1:1065342/1‑140 (MQ=255)                                                                                   gACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAAt                                                   <  1:129391/140‑1 (MQ=255)                                                                                     cGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAg                                                            <  2:753897/129‑1 (MQ=255)                                                                                     cGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAg                                                            >  1:753897/1‑129 (MQ=255)                                                                                     cGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCg                                                 >  1:76570/1‑140 (MQ=255)                                                                                       tCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGt                                               <  2:244431/140‑1 (MQ=255)                                                                                       tCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGt                                               <  2:838746/140‑1 (MQ=255)                                                                                       tCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGt                                               <  2:646598/140‑1 (MQ=255)                                                                                       tCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGt                                               >  1:505283/1‑140 (MQ=255)                                                                                       tCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGt                                               <  1:526488/140‑1 (MQ=255)                                                                                       tCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGt                                               <  1:547596/140‑1 (MQ=255)                                                                                       tCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGt                                               <  2:33665/140‑1 (MQ=255)                                                                                       tCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGt                                               >  2:29716/1‑140 (MQ=255)                                                                                        cGAAATCGAGATATCTCTCCAGTCTCCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGtt                                              <  1:231916/140‑1 (MQ=255)                                                                                          aaaTCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTgg                                            >  1:196052/1‑140 (MQ=255)                                                                                            aTCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGcc                                          <  2:92267/140‑1 (MQ=255)                                                                                              cGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCa                                        <  2:321800/140‑1 (MQ=255)                                                                                              cGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCa                                        <  2:879992/140‑1 (MQ=255)                                                                                                  atatCTCTCCAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGa                                    <  1:365354/140‑1 (MQ=255)                                                                                                          ccAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGa                            >  1:885735/1‑140 (MQ=255)                                                                                                           cAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGAt                           >  2:280893/1‑140 (MQ=255)                                                                                                           cAGTCACCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGAt                           <  1:223931/140‑1 (MQ=255)                                                                                                                aCCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCt                      >  2:272283/1‑140 (MQ=255)                                                                                                                aCCAATATCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCt                      >  1:154745/1‑140 (MQ=255)                                                                                                                   aaTATCAACATTAAAACCTTCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGtt                   >  1:720727/1‑140 (MQ=255)                                                                                                                     tatCAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGtttt                 <  1:218510/140‑1 (MQ=255)                                                                                                                        cAACATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCg              >  1:59940/1‑140 (MQ=255)                                                                                                                          aCATTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTc            <  2:1065342/140‑1 (MQ=255)                                                                                                                            aTTAAAACCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCt          >  1:706234/1‑140 (MQ=255)                                                                                                                               aaaaCCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTgcg       <  1:272283/140‑1 (MQ=255)                                                                                                                                  aCCTGCCGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCAt    <  2:885735/140‑1 (MQ=255)                                                                                                                                    ctgccgTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCg  <  1:589755/140‑1 (MQ=255)                                                                                                                                    ctgccgTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCg  <  2:12710/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        ACCACCGTTACCGAAGGGGAGCGAAATCGACAGATAAATCTGGTTGTCGCGATCTTTATCTTCGTAGTGGGTCGTATTGAATGACGTCGAAATCGAGATATCTCTCCAGTCACCAATATCAACATTAAAACCTGCTGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCG  >  NC_000913/750490‑750760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑