Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	808,482	G→T	A259D (GCC→GAC)	bioA ←	adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	808,482	0	G	T	100.0%	81.4 / NA	24	A259D (GCC→GAC)	bioA	adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/11);  total (13/11)

TTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCC  >  NC_000913/808365‑808602                                                                                                                      |                                                                                                                         ttACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGAcc                                                                                                    <  2:581820/140‑1 (MQ=255)       aTGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATcc                                                                                              >  1:352594/1‑140 (MQ=255)         ggTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAg                                                                                            <  1:646712/140‑1 (MQ=255)            ttCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTgg                                                                                         >  1:1057987/1‑140 (MQ=255)                    ccTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCtcg                                                                                 >  1:397271/1‑140 (MQ=255)                        gcgcgTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCgg                                                                             <  2:397271/140‑1 (MQ=255)                         cgcgTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGc                                                                            <  2:352594/140‑1 (MQ=255)                                    tgtgGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGa                                                                 <  2:163662/140‑1 (MQ=255)                                        gCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATAcc                                                             >  1:369935/1‑140 (MQ=255)                                         cGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCt                                                            >  2:767174/1‑140 (MQ=255)                                                  tCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCg                                                   >  1:1068838/1‑140 (MQ=255)                                                          ccgccgGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATAtttt                                           >  1:734444/1‑140 (MQ=255)                                                                gTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGAt                                     <  2:20894/140‑1 (MQ=255)                                                                          tACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAAc                           >  2:97271/1‑140 (MQ=255)                                                                              gtggCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCAtt                       <  2:734444/138‑1 (MQ=255)                                                                                ggCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTcc                     >  2:51049/1‑140 (MQ=255)                                                                                   acaAAATGTCCGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGa                  <  1:964290/140‑1 (MQ=255)                                                                                   acaAAATGTCCGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGa                  <  1:97271/140‑1 (MQ=255)                                                                                           tCCGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACAt          >  2:300975/1‑140 (MQ=255)                                                                                            ccGGCGCGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGCACATg         <  2:369935/140‑1 (MQ=255)                                                                                               gcgcGATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATgcgc      >  1:100971/1‑140 (MQ=255)                                                                                                   gATTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCTGATGGTACATGCGCATcc  >  1:669043/1‑140 (MQ=255)                                                                                                    aTTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCAt    <  1:937605/137‑1 (MQ=255)                                                                                                    aTTTCTGCATGTTCACAGTCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCAt    >  2:937605/1‑137 (MQ=255)                                                                                                                      |                                                                                                                         TTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCC  >  NC_000913/808365‑808602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑