Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	45,294	A→G	N372S (AAC→AGC)	fixC →	putative oxidoreductase FixC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	45,294	0	A	G	100.0%	191.7 / NA	54	N372S (AAC→AGC)	fixC	putative oxidoreductase FixC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/28);  total (26/28)

CAGCAAAAACCGTGCTTTCAGCGATGAAAAGCGACGATTTCAGTAAGCAAAAACTGGCGGAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAACCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATC  >  NC_000913/45159‑45428                                                                                                                                        |                                                                                                                                       cagcaAAAACCGTGCTTTCAGCGATGAAAAGCGACGATTTCAGTAAGCAAAAACTGGCGGAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCa                                                                                                                                    <  1:188276/140‑1 (MQ=255)  agcaAAAACCGTGCTTTCAGCGATGAAAAGCGACGATTTCAGTAAGCAAAAACTGGCGGAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCAc                                                                                                                                   >  2:926153/1‑140 (MQ=255)   gcaAAAACCGTGCTTTCAGCGATGAAAAGCGACGATTTCAGTAAGCAAAAACTGGCGGAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACg                                                                                                                                  <  1:607142/140‑1 (MQ=255)            gTGCTTTCAGCGATGAAAAGCGACGATTTCAGTAAGCAAAAACTGGCGGAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAg                                                                                                                         <  2:878571/140‑1 (MQ=255)            gTGCTTTCAGCGATGAAAAGCGACGATTTCAGTAAGCAAAAACTGGCGGAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAg                                                                                                                         <  1:975617/140‑1 (MQ=255)                 ttCAGCGATGAAAAGCGACGATTTCAGTAAGCAAAAACTGGCGGAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCt                                                                                                                    >  2:551843/1‑140 (MQ=255)                              aGCGACGATTTCAGTAAGCAAAAACTGGCGGAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGc                                                                                                       >  1:933112/1‑140 (MQ=255)                                 gacgaTTTCAGTAAGCAAAAACTGGCGGAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCggt                                                                                                    <  2:933112/140‑1 (MQ=255)                                 gacgaTTTCAGTAAGCAAAAACTGGCGGAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCggt                                                                                                    <  1:926153/140‑1 (MQ=255)                                 gacgaTTTCAGTAAGCAAAAACTGGCGGAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCggc                                                                                                    <  2:233258/140‑2 (MQ=255)                                      tttCAGTAAGCAAAAACTGGCGGAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCggtgggtg                                                                                               <  2:643962/140‑1 (MQ=255)                                       ttCAGTAAGCAAAAACTGGCGGAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGgtgt                                                                                              >  2:807457/1‑140 (MQ=255)                                            tAAGCAAAAACTGGCGGAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGgcgc                                                                                         <  1:1002593/140‑1 (MQ=255)                                                cAAAAACTGGCGGAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGAGGCGCGTGa                                                                                     >  2:453742/1‑140 (MQ=255)                                                 aaaaaCTGGCGGAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGAc                                                                                    <  2:606374/140‑1 (MQ=255)                                                      cTGGCGGAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGtt                                                                               <  2:1034142/140‑1 (MQ=255)                                                          cGGAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCAcc                                                                           <  1:551843/140‑1 (MQ=255)                                                             aaTATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCAtt                                                                        >  2:748370/1‑140 (MQ=255)                                                                          tCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAgcgcgc                                                           <  1:83724/140‑1 (MQ=255)                                                                                  gTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTg                                                   >  2:36273/1‑140 (MQ=255)                                                                                   tGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGa                                                  >  1:782908/1‑140 (MQ=255)                                                                                   tGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGa                                                  >  1:413395/1‑140 (MQ=255)                                                                                     gTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAg                                                                >  1:323653/1‑124 (MQ=255)                                                                                     gTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAg                                                                <  2:323653/124‑1 (MQ=255)                                                                                     gTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATg                                                <  1:898563/140‑1 (MQ=255)                                                                                     gTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATg                                                >  1:663596/1‑140 (MQ=255)                                                                                     gTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATg                                                >  1:971383/1‑140 (MQ=255)                                                                                     gTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATg                                                >  2:547672/1‑140 (MQ=255)                                                                                     gTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATg                                                >  2:449816/1‑140 (MQ=255)                                                                                     gTCCGCTGCGCGATATGCATATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGGAGCGCGCCGGGACTGATg                                                >  2:639707/1‑140 (MQ=255)                                                                                      tCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCgg                                                        <  2:340887/131‑1 (MQ=255)                                                                                      tCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCgg                                                        >  1:340887/1‑131 (MQ=255)                                                                                         gCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCa                                            <  1:36273/140‑1 (MQ=255)                                                                                               cgATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGaaaa                                      >  2:251253/1‑140 (MQ=255)                                                                                                  tatGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAAtcc                                   <  2:782908/140‑1 (MQ=255)                                                                                                       gTATGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGc                              <  1:95517/140‑1 (MQ=255)                                                                                                         aTGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCa                            <  2:2196/140‑1 (MQ=255)                                                                                                         aTGTACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCa                            <  2:118366/140‑1 (MQ=255)                                                                                                            tACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAGATCCTCCGCCACgg                         >  2:661043/1‑140 (MQ=255)                                                                                                            tACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACgg                         >  2:249190/1‑140 (MQ=255)                                                                                                            tACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACgg                         >  2:629544/1‑140 (MQ=255)                                                                                                            tACCAGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTAACCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACgg                         <  2:971383/140‑1 (MQ=255)                                                                                                                aGAAACTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGGGCGGCGCGTGACCCATTCACCATTGATGGCAGCGCGCCGGCACTGATGCGCAAGAAAATCCCCCGCCACGGCCAg                     >  2:725200/1‑140 (MQ=255)                                                                                                                    aCTACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAg                 <  2:838218/140‑1 (MQ=255)                                                                                                                      tACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTg               >  1:768235/1‑140 (MQ=255)                                                                                                                      tACCGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTg               >  1:239586/1‑140 (MQ=255)                                                                                                                        ccGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTggg             >  2:238110/1‑140 (MQ=255)                                                                                                                        ccGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTggg             <  1:629544/140‑1 (MQ=255)                                                                                                                         cGGCGTTCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGc            >  1:364772/1‑140 (MQ=255)                                                                                                                              ttCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTtcat       <  2:802548/140‑1 (MQ=255)                                                                                                                              ttCCTTGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGAGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTtcat       >  2:75729/1‑140 (MQ=255)                                                                                                                                  ttGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAAt   <  2:228484/140‑1 (MQ=255)                                                                                                                                  ttGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAAt   <  1:251253/140‑1 (MQ=255)                                                                                                                                   tGATAGCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATc  <  2:102961/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       CAGCAAAAACCGTGCTTTCAGCGATGAAAAGCGACGATTTCAGTAAGCAAAAACTGGCGGAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAACCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATC  >  NC_000913/45159‑45428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑