Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,013,628	T→C	S124P (TCT→CCT)	pqiB →	intermembrane transport protein PqiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,013,628	0	T	C	100.0%	136.5 / NA	39	S124P (TCT→CCT)	pqiB	intermembrane transport protein PqiB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/19);  total (20/19)

GATTTGACGCACGTTGAAATCAAAGCGCGGCTGAATTCCGGTATGGAAAAATTGCTGCATAAAGACACCGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGG  >  NC_000913/1013496‑1013761                                                                                                                                     |                                                                                                                                      gatTTGACGCACGTTGAAATCAAAGCGCGGCTGAATTCCGGTATGGAAAAATTGCTGCATAAAGACACCGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGt                                                                                                                                <  1:562868/140‑1 (MQ=255)     tGACGCACGTTGAAATCAAAGCGCGGCTGAATTCCGGTATGGAAAAATTGCTGCATAAAGACACCGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTtat                                                                                                                            >  1:947032/1‑140 (MQ=255)           aCGTTGAAATCAAAGCGCGGCTGAATTCCGGTATGGAAAAATTGCTGCATAAAGACACCGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGaa                                                                                                                      >  2:632591/1‑140 (MQ=255)                  aaTCAAAGCGCGGCTGAATTCCGGTATGGAAAAATTGCTGCATAAAGACACCGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGc                                                                                                               <  1:256557/140‑1 (MQ=255)                  aaTCAAAGCGCGGCTGAATTCCGGTATGGAAAAATTGCTGCATAAAGACACCGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGc                                                                                                               <  2:118861/140‑1 (MQ=255)                       aaGCGCGGCTGAATTCCGGTATGGAAAAATTGCTGCATAAAGACACCGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGgc                                                                                                          >  2:699843/1‑140 (MQ=255)                       aaGCGCGGCTGAATTCCGGTATGGAAAAATTGCTGCATAAAGACACCGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGgc                                                                                                          >  1:853729/1‑140 (MQ=255)                          cgcgGCTGAATTCCGGTATGGAAAAATTGCTGCATAAAGACACCGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGcgcg                                                                                                       <  1:632591/140‑1 (MQ=255)                          cgcgGCTGAATTCCGGTATGGAAAAATTGCTGCATAAAGACACCGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGcgcg                                                                                                       <  2:619147/140‑1 (MQ=255)                           gcgGCTGAATTCCGGTATGGAAAAATTGCTGCATAAAGACACCGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGa                                                                                                      >  1:784197/1‑140 (MQ=255)                             ggCTGAATTCCGGTATGGAAAAATTGCTGCATAAAGACACCGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGTCAGGCGCGaaa                                                                                                    >  2:650025/1‑140 (MQ=255)                                  aaTTCCGGTATGGAAAAATTGCTGCATAAAGACACCGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGgcag                                                                                               <  2:852940/140‑1 (MQ=255)                                  aaTTCCGGTATGGAAAAATTGCTGCATAAAGACACCGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGgcag                                                                                               <  1:641876/140‑1 (MQ=255)                                                                  cacCGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGc                                                               <  2:947032/140‑1 (MQ=255)                                                                       tctcttGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGc                                                               >  2:185829/5‑135 (MQ=255)                                                                       tctcTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGc                                                               <  1:185829/135‑1 (MQ=255)                                                                       tCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCg                                                          >  1:673602/1‑140 (MQ=255)                                                                       tCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCg                                                          <  1:699843/140‑1 (MQ=255)                                                                         ttttGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGtt                                                        >  1:560023/1‑140 (MQ=255)                                                                          tttGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCg                                                                <  1:91207/131‑1 (MQ=255)                                                                          tttGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCg                                                                >  2:91207/1‑131 (MQ=255)                                                                                 ggtgAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGc                                                >  2:496281/1‑140 (MQ=255)                                                                                  gtgAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGcc                                               >  1:539893/1‑140 (MQ=255)                                                                                  gtgAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGcc                                               >  1:260782/1‑140 (MQ=255)                                                                                  gtgAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGcc                                               <  2:673602/140‑1 (MQ=255)                                                                                   tgAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCt                                              <  1:508346/140‑1 (MQ=255)                                                                                   tgAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCt                                              >  2:508346/1‑140 (MQ=255)                                                                                    gAAACAGAAGATGGGTCGCGAAGGGATTAGCGGCCTGGGAACCCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTCCTGCACTCGCCACCGTTCGCCCCGCCTg                                             <  2:668478/140‑1 (MQ=255)                                                                                       aCCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATg                                          <  2:784197/140‑1 (MQ=255)                                                                                         cGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCg                                        <  2:185722/140‑1 (MQ=255)                                                                                             gATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAg                                    >  2:34190/1‑140 (MQ=255)                                                                                                     gcgAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCgt                            >  1:27270/1‑140 (MQ=255)                                                                                                      cgAAGGGATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCgtg                           <  1:934074/140‑1 (MQ=255)                                                                                                           ggATTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGAtt                      <  2:260782/140‑1 (MQ=255)                                                                                                             aTTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCgtg                           <  2:76190/133‑1 (MQ=255)                                                                                                             aTTAGCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCgtg                           >  1:76190/1‑133 (MQ=255)                                                                                                                 gCGGCCTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGTCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGAt                <  1:650025/140‑1 (MQ=255)                                                                                                                     ccTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCa            >  1:862796/1‑140 (MQ=255)                                                                                                                      cTGGGAACGCTGCTGCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCaa           >  2:422176/1‑140 (MQ=255)                                                                                                                               ctgctgCCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCgg  >  2:433007/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      GATTTGACGCACGTTGAAATCAAAGCGCGGCTGAATTCCGGTATGGAAAAATTGCTGCATAAAGACACCGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGG  >  NC_000913/1013496‑1013761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑