Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,134,819	A→G	T31A (ACT→GCT)	flgG →	flagellar basal‑body rod protein FlgG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,134,819	0	A	G	100.0%	299.3 / NA	82	T31A (ACT→GCT)	flgG	flagellar basal‑body rod protein FlgG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/38);  total (44/38)

AGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGT  >  NC_000913/1134685‑1134952                                                                                                                                       |                                                                                                                                      agaagaCATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAAc                                                                                                                                  >  2:643881/1‑140 (MQ=255)  gaagaCATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACg                                                                                                                                 >  1:1076403/1‑140 (MQ=255)         tatGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTaa                                                                                                                          >  2:388013/1‑140 (MQ=255)            gCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAAc                                                                                                                                  <  2:955070/129‑1 (MQ=255)            gCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAAc                                                                                                                                  >  1:955070/1‑129 (MQ=255)            gCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCg                                                                                                                       >  1:598933/1‑140 (MQ=255)             cACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGt                                                                                                                      >  1:45368/1‑140 (MQ=255)             cACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGAAAACGTCAGTGCTAACGGTTTTAAGCGt                                                                                                                      >  1:910122/1‑140 (MQ=255)                tttGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAg                                                                                                                   >  1:173758/1‑140 (MQ=255)                tttGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAg                                                                                                                   >  2:512913/1‑140 (MQ=255)                tttGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAg                                                                                                                   >  2:151743/1‑140 (MQ=255)                   gTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAgcgc                                                                                                                <  1:600799/140‑1 (MQ=255)                   gTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAgcgc                                                                                                                <  2:646019/140‑1 (MQ=255)                    tCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAgcgcg                                                                                                               <  2:682659/140‑1 (MQ=255)                    tCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAgcgcg                                                                                                               >  1:7161/1‑140 (MQ=255)                     cACTAATCCACTACAGGACATTTTGTGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAgcgcgc                                                                                                              <  2:908473/140‑1 (MQ=255)                     cACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAgcgcgc                                                                                                              <  2:173758/140‑1 (MQ=255)                      aCTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGcgcgcg                                                                                                             <  1:388013/140‑1 (MQ=255)                      aCTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGcgcgcg                                                                                                             <  1:643881/140‑1 (MQ=255)                        tAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGgt                                                                                                           <  2:168628/140‑1 (MQ=255)                          aTCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAgcgcg                                                                                                               >  2:11364/1‑134 (MQ=255)                          aTCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAgcgcg                                                                                                               <  1:11364/134‑1 (MQ=255)                              aCTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGcgcgcg                                                                                                             >  2:384311/1‑132 (MQ=255)                              aCTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGcgcgcg                                                                                                             <  1:384311/132‑1 (MQ=255)                              aCTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGa                                                                                                     >  2:137069/1‑140 (MQ=255)                                tACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAg                                                                                                   >  1:584775/1‑140 (MQ=255)                                     gACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAgcgcg                                                                                                               <  1:1074593/123‑1 (MQ=255)                                     gACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAgcgcg                                                                                                               >  2:1074593/1‑123 (MQ=255)                                       cATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCt                                                                                            <  2:882678/140‑1 (MQ=255)                                          tttATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTa                                                                                         >  2:667041/1‑140 (MQ=255)                                          tttATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTa                                                                                         >  2:338237/1‑140 (MQ=255)                                            tATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATc                                                                                       >  2:377776/1‑140 (MQ=255)                                              tGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCaa                                                                                     >  1:161613/1‑140 (MQ=255)                                                  cAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCa                                                                                 >  2:372339/1‑140 (MQ=255)                                                  cAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCa                                                                                 >  2:980030/1‑140 (MQ=255)                                                  cAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCa                                                                                 <  1:338237/140‑1 (MQ=255)                                                  cAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCa                                                                                 <  1:602619/140‑1 (MQ=255)                                                    gTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCAtt                                                                               >  2:435229/1‑140 (MQ=255)                                                    gTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCAtt                                                                               >  1:878966/1‑140 (MQ=255)                                                    gTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCAtt                                                                               >  1:839195/1‑140 (MQ=255)                                                    gTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCAtt                                                                               >  1:702998/1‑140 (MQ=255)                                                     ttCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTc                                                                              >  1:654302/1‑140 (MQ=255)                                                     ttCATTATGGATCGCCAAAACGGGCCTAGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTc                                                                              >  1:481198/1‑140 (MQ=255)                                                         ttATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCa                                                                          <  2:839195/140‑1 (MQ=255)                                                         ttATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCa                                                                          <  1:512913/140‑1 (MQ=255)                                                          tATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAg                                                                         >  2:880087/1‑140 (MQ=255)                                                           aTGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGc                                                                        <  1:743692/140‑1 (MQ=255)                                                             ggATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCg                                                                      <  2:7161/140‑1 (MQ=255)                                                             ggATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCg                                                                      >  2:781811/1‑140 (MQ=255)                                                               aTCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCggg                                                                    >  1:744473/1‑140 (MQ=255)                                                                 cGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCgg                                                                     >  1:815626/1‑137 (MQ=255)                                                                 cGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCgg                                                                     <  2:815626/137‑1 (MQ=255)                                                                  gCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGc                                                                 >  2:69376/1‑140 (MQ=255)                                                                  gCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGc                                                                 >  1:634704/1‑140 (MQ=255)                                                                   ccAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGca                                                                <  1:980030/140‑1 (MQ=255)                                                                   ccAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGca                                                                <  1:164140/140‑1 (MQ=255)                                                                   ccAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGca                                                                >  1:914463/1‑140 (MQ=255)                                                                   ccAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGca                                                                <  1:372339/140‑1 (MQ=255)                                                                     aaaaCGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGcaca                                                              <  1:69376/140‑1 (MQ=255)                                                                            gCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTcc                                                       >  2:491500/1‑140 (MQ=255)                                                                               ttGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGaa                                                    <  2:584775/140‑1 (MQ=255)                                                                                  aCGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACaa                                                 >  2:625773/1‑140 (MQ=255)                                                                                   cGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACaaa                                                <  1:761438/140‑1 (MQ=255)                                                                                   cGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACaaa                                                <  2:1076403/140‑1 (MQ=255)                                                                                   cGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACaaa                                                <  1:137069/140‑1 (MQ=255)                                                                                   cGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGAGCACAGTCTTCCGAACaaa                                                <  2:654302/140‑1 (MQ=255)                                                                                      ccAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAacca                                             <  2:45368/140‑1 (MQ=255)                                                                                             aCCAATATGGACGTCATTGACAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTAcc                                      <  2:498764/140‑1 (MQ=255)                                                                                                aaTATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTc                                   <  1:435229/140‑1 (MQ=255)                                                                                                aaTATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTc                                   >  1:859496/1‑140 (MQ=255)                                                                                                            aTTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAAt                       >  1:437681/1‑140 (MQ=255)                                                                                                            aTTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAAt                       >  2:793271/1‑140 (MQ=255)                                                                                                            aTTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAAt                       <  2:281721/140‑1 (MQ=255)                                                                                                            aTTGCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAAt                       >  1:317296/1‑140 (MQ=255)                                                                                                               gCCAACAACCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCgg                    <  2:317296/140‑1 (MQ=255)                                                                                                                  aacaacCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCAc                 <  2:925856/140‑1 (MQ=255)                                                                                                                   acaacCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACg                <  2:598933/140‑1 (MQ=255)                                                                                                                    caacCTGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACgg               >  2:31128/1‑140 (MQ=255)                                                                                                                         tGGCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTa          <  2:859496/140‑1 (MQ=255)                                                                                                                          ggCAAACGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTAc         >  1:1077033/1‑140 (MQ=255)                                                                                                                                cGTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCgg   >  2:304998/1‑140 (MQ=255)                                                                                                                                 gTCAGTGCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGt  <  2:910122/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      AGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGT  >  NC_000913/1134685‑1134952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑