Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,167,064	T→C	V327A (GTT→GCT)	ndh →	NADH:quinone oxidoreductase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,167,064	0	T	C	100.0%	184.3 / NA	52	V327A (GTT→GCT)	ndh	NADH:quinone oxidoreductase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/36);  total (16/36)

CTGAAAGATATCGGTGGTCTTGAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTATCAGTATAAAGATCATGGTTCGCTGGTATCGCTGTCGAACTT  >  NC_000913/1166928‑1167196                                                                                                                                         |                                                                                                                                     cTGAAAGATATCGGTGGTCTTGAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTcc                                                                                                                                   <  1:925644/140‑1 (MQ=255)     aaGATATCGGTGGTCTTGAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTccgccg                                                                                                                               <  1:510221/140‑1 (MQ=255)            cGGTGGTCTTGAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTc                                                                                                                        <  1:938724/140‑1 (MQ=255)             ggtggtCTGGAAACTAACCGTAGCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCCCCCGAGTTCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCa                                                                                                                       <  2:1039198/140‑1 (MQ=255)              gtggtCTTGAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGc                                                                                                                          >  1:799887/1‑136 (MQ=255)              gtggtCTTGAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGc                                                                                                                          <  2:799887/136‑1 (MQ=255)               tggtCTTGAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAgg                                                                                                                     <  2:552104/140‑1 (MQ=255)               tggtCTTGAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAgg                                                                                                                     <  1:435420/140‑1 (MQ=255)                 gtCTTGAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTc                                                                                                                        <  2:316017/135‑1 (MQ=255)                 gtCTTGAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTc                                                                                                                        >  1:316017/1‑135 (MQ=255)                 gtCTTGAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCt                                                                                                                   <  2:917593/140‑1 (MQ=255)                       aaaCTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCacac                                                                                                             <  1:502918/140‑1 (MQ=255)                               cGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGc                                                                                                     <  2:738033/140‑1 (MQ=255)                                       ccAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTgcg                                                                                             <  2:471114/140‑1 (MQ=255)                                       ccAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTgcg                                                                                             >  1:471114/1‑140 (MQ=255)                                         aGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTg                                                                                               >  2:1002861/1‑136 (MQ=255)                                         aGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTg                                                                                               <  1:1002861/136‑1 (MQ=255)                                         aGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCa                                                                                           >  1:975475/1‑140 (MQ=255)                                         aGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCa                                                                                           >  2:293894/1‑140 (MQ=255)                                         aGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCa                                                                                           <  1:894852/140‑1 (MQ=255)                                         aGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCa                                                                                           <  1:611780/140‑1 (MQ=255)                                          gCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGGCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCaa                                                                                          >  2:118666/1‑140 (MQ=255)                                                     aaCCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACAtt                                                                               >  2:290841/1‑140 (MQ=255)                                                      aCCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTc                                                                              <  1:855032/140‑1 (MQ=255)                                                      aCCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTc                                                                              <  1:293894/140‑1 (MQ=255)                                                         gACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTgg                                                                           >  1:234475/1‑140 (MQ=255)                                                              tGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGTGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAg                                                                      <  2:744353/140‑1 (MQ=255)                                                              tGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGGCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAg                                                                      <  1:118666/140‑1 (MQ=255)                                                                cAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCTGAAGGGGGCTTTGCTCCGCCGCGTGCCCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGAt                                                                    <  2:128640/140‑1 (MQ=255)                                                                 aaaCCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATg                                                                   <  1:375043/140‑1 (MQ=255)                                                                       cccGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCTGATGAACGGt                                                             <  1:768977/140‑1 (MQ=255)                                                                             aTCTAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCg                                                       <  2:535386/140‑1 (MQ=255)                                                                                        tACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTa                                            >  1:102972/1‑140 (MQ=255)                                                                                        tACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTa                                            >  2:444002/1‑140 (MQ=255)                                                                                         aCGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCGGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTAt                                           <  2:400804/140‑1 (MQ=255)                                                                                         aCGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTAt                                           <  1:328538/140‑1 (MQ=255)                                                                                         aCGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTAt                                           <  1:683101/140‑1 (MQ=255)                                                                                         aCGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTAt                                           <  1:244537/140‑1 (MQ=255)                                                                                         aCGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTAGCGCAGATGAACGGTAAGCCGCTGAAAAATTAt                                           <  2:851632/140‑1 (MQ=255)                                                                                               ttGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTAt                                           <  1:337103/134‑1 (MQ=255)                                                                                               ttGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTAt                                           >  2:337103/1‑134 (MQ=255)                                                                                               ttGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTATCAGtat                                     <  1:332558/140‑1 (MQ=255)                                                                                               ttGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTATCAGtat                                     <  1:932163/140‑1 (MQ=255)                                                                                               ttGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTATCAGtat                                     >  1:801172/1‑140 (MQ=255)                                                                                                       tGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTATCAGTATAAAGATCa                             <  2:1040896/140‑1 (MQ=255)                                                                                                         cgcgTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTAt                                           <  2:443835/124‑1 (MQ=255)                                                                                                         cgcgTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTAt                                           >  1:443835/1‑124 (MQ=255)                                                                                                         cgcgTCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTATCAGTATAAAGATCATg                           >  1:308008/1‑140 (MQ=255)                                                                                                             tCATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTATCAGTATAAAGATCATGGTTc                       <  1:444002/140‑1 (MQ=255)                                                                                                              cATGCCCGCGTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTATCAGTATAAAGATCATGGTTCg                      <  2:234475/140‑1 (MQ=255)                                                                                                                       gTCCGGAAGGGGGCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTATCAGTATAAAGATCATGGTTCGCTGGTATCg             <  1:1074939/140‑1 (MQ=255)                                                                                                                                  ggCTTTGCTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTATCAGTATAAAGATCATGGTTCGCTGGTATCGCTGTCGAACtt  >  1:805489/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     CTGAAAGATATCGGTGGTCTTGAAACTAACCGTATCAACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCGCGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGCGCAGATGAACGGTAAGCCGCTGAAAAATTATCAGTATAAAGATCATGGTTCGCTGGTATCGCTGTCGAACTT  >  NC_000913/1166928‑1167196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑