Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,193,012	A→G	G254G (GGT→GGC)	mnmA ←	tRNA‑specific 2‑thiouridylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,193,012	0	A	G	100.0%	122.7 / NA	35	G254G (GGT→GGC)	mnmA	tRNA‑specific 2‑thiouridylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/18);  total (17/18)

TTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGGC  >  NC_000913/1192880‑1193142                                                                                                                                     |                                                                                                                                   ttCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCcttctt                                                                                                                             >  2:957458/1‑140 (MQ=255)          gACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCAc                                                                                                                    <  2:140266/140‑1 (MQ=255)                gTGCAACTGCTGGGCAATCAACCCGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATAc                                                                                                              >  1:16064/1‑140 (MQ=255)                     aCTGCTGGGCAATCAACCCGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGa                                                                                                         <  2:623834/140‑1 (MQ=255)                      ctgctgGGCAATCAACCCGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGAc                                                                                                        >  1:618304/1‑140 (MQ=255)                           gggCAATCAACCCGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGATTGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTa                                                                                                   >  2:751121/1‑140 (MQ=255)                                  cAACCCGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGAc                                                                                            <  2:618304/140‑1 (MQ=255)                                  cAACCCGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGAc                                                                                            <  1:933852/140‑1 (MQ=255)                                   aaCCCGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGAcc                                                                                           <  1:361661/140‑1 (MQ=255)                                       cGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCgaga                                                                                       <  2:834982/140‑1 (MQ=255)                                               aTCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATa                                                                               >  1:85934/1‑140 (MQ=255)                                                  aGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACAt                                                                            <  1:957458/140‑1 (MQ=255)                                                   gCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATc                                                                           >  2:591611/1‑140 (MQ=255)                                                         ggTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGcccc                                                                     <  1:591611/140‑1 (MQ=255)                                                                    cccTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCAcc                                                          >  2:167310/1‑140 (MQ=255)                                                                     ccTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCa                                                         >  2:525142/1‑140 (MQ=255)                                                                       tGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAAt                                                       >  1:630630/1‑140 (MQ=255)                                                                        gAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGcccc                                                                     >  2:532434/1‑125 (MQ=255)                                                                        gAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGcccc                                                                     <  1:532434/125‑1 (MQ=255)                                                                        gAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAAtt                                                      >  1:407585/1‑140 (MQ=255)                                                                         aGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGc                                                               >  1:288256/1‑130 (MQ=255)                                                                         aGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGc                                                               <  2:288256/130‑1 (MQ=255)                                                                         aGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAAttt                                                     >  2:649050/1‑140 (MQ=255)                                                                                aCCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCGcc                                              <  1:649050/140‑1 (MQ=255)                                                                                              ttCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCGCCATCGACGGTAATGa                                <  1:531723/140‑1 (MQ=255)                                                                                              ttCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCGCCATCGACGGTAATGa                                <  2:407585/140‑1 (MQ=255)                                                                                                         tGTCCACCACATACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCGCCATCGACGGTAATGATTTTGCCCGGt                     <  2:85934/140‑1 (MQ=255)                                                                                                                 acaTACCACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCgg             <  1:118321/140‑1 (MQ=255)                                                                                                                       cACGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATa       <  2:690200/140‑1 (MQ=255)                                                                                                                         cGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATaa      >  2:721487/1‑139 (MQ=255)                                                                                                                         cGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATaa      <  1:721487/139‑1 (MQ=255)                                                                                                                         cGGTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAAc     <  1:305672/140‑1 (MQ=255)                                                                                                                          ggTTCTTCGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACg    >  1:724797/1‑140 (MQ=255)                                                                                                                            ttcttcGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGGc  >  2:786074/1‑140 (MQ=255)                                                                                                                            ttcttcGGTGCCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGGc  >  1:828660/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                   TTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAATTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGGC  >  NC_000913/1192880‑1193142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑