Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,258,763	A→G	intergenic (‑250/‑28)	pth ← / → ychH	peptidyl‑tRNA hydrolase/stress‑induced protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,258,763	0	A	G	100.0%	312.5 / NA	86	intergenic (‑250/‑28)	pth/ychH	peptidyl‑tRNA hydrolase/stress‑induced protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/42);  total (44/42)

TAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTT  >  NC_000913/1258629‑1258898                                                                                                                                       |                                                                                                                                        tAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAAcc                                                                                                                                    >  2:981372/1‑140 (MQ=255) tAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAAcc                                                                                                                                    >  2:946806/1‑140 (MQ=255)      aGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGaa                                                                                                                               >  1:951619/1‑140 (MQ=255)      aGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGaa                                                                                                                               <  1:981372/140‑1 (MQ=255)        tCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAg                                                                                                                             >  2:554697/1‑140 (MQ=255)        tCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAg                                                                                                                             >  1:675622/1‑140 (MQ=255)          aaGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGtt                                                                                                                           <  1:1040699/140‑1 (MQ=255)          aaGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGtt                                                                                                                           >  1:315930/1‑140 (MQ=255)          aaGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGtt                                                                                                                           <  1:986509/140‑1 (MQ=255)          aaGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGtt                                                                                                                           >  2:512448/1‑140 (MQ=255)           aGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTc                                                                                                                          >  2:816424/1‑140 (MQ=255)                ttCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAg                                                                                                                     <  1:1057101/140‑1 (MQ=255)                        tttATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGt                                                                                                                   <  2:400781/134‑1 (MQ=255)                        tttATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGt                                                                                                                   >  1:400781/1‑134 (MQ=255)                          tATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGt                                                                                                                   >  1:646262/1‑132 (MQ=255)                          tATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGt                                                                                                                   <  2:646262/132‑1 (MQ=255)                          tATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATg                                                                                                           >  2:822170/1‑140 (MQ=255)                             ttGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGaaa                                                                                                        <  1:527762/140‑1 (MQ=255)                                          ttgAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCtt                                                                                           <  2:766192/140‑1 (MQ=255)                                          ttgAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCtt                                                                                           >  1:357620/1‑140 (MQ=255)                                            gAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCg                                                                                         <  1:326161/140‑1 (MQ=255)                                            gAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCg                                                                                         <  2:707043/140‑1 (MQ=255)                                                       tGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTaa                                                                              <  1:822170/140‑1 (MQ=255)                                                       tGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTaa                                                                              >  2:708301/1‑140 (MQ=255)                                                         tAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGtt                                                                                       <  1:890114/129‑1 (MQ=255)                                                         tAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGtt                                                                                       >  2:890114/1‑129 (MQ=255)                                                         tAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACg                                                                            >  2:356329/1‑140 (MQ=255)                                                         tAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACg                                                                            <  1:946806/140‑1 (MQ=255)                                                         tAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACg                                                                            <  1:808552/140‑1 (MQ=255)                                                           aTTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTg                                                                          <  2:951619/140‑1 (MQ=255)                                                           aTTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTg                                                                          <  2:315930/140‑1 (MQ=255)                                                              gtgATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACg                                                                            <  1:25545/135‑1 (MQ=255)                                                              gtgATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACg                                                                            >  2:25545/1‑135 (MQ=255)                                                               tgATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCa                                                                      >  2:143655/1‑140 (MQ=255)                                                                gATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAAc                                                                             >  1:381829/1‑132 (MQ=255)                                                                gATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAAc                                                                             <  2:381829/132‑1 (MQ=255)                                                                gATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCAt                                                                     >  1:917730/1‑140 (MQ=255)                                                                      gCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGtt                                                               >  1:553619/1‑140 (MQ=255)                                                                      gCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGtt                                                               >  1:365394/1‑140 (MQ=255)                                                                         cGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTggg                                                            >  1:973054/1‑140 (MQ=255)                                                                          gCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGt                                                           <  1:816424/140‑1 (MQ=255)                                                                            acaTAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCt                                                         <  2:917730/140‑1 (MQ=255)                                                                             caTAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCtg                                                        <  2:553619/140‑1 (MQ=255)                                                                                aaCCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCtggtg                                                     <  2:365394/140‑1 (MQ=255)                                                                                      cTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAAtg                                               >  2:482272/1‑140 (MQ=255)                                                                                      cTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAAtg                                               >  1:202469/1‑140 (MQ=255)                                                                                      cTGGGTGTTGTCCATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAAtg                                               >  2:717366/1‑140 (MQ=255)                                                                                        gggTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCt                                                         >  2:503886/1‑128 (MQ=255)                                                                                        gggTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCt                                                         <  1:503886/128‑1 (MQ=255)                                                                                           tgttgtCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATggtggt                                          <  1:59095/140‑1 (MQ=255)                                                                                           tgttgtCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATggtggt                                          <  1:482272/140‑1 (MQ=255)                                                                                           tgttgtCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATggtggt                                          <  1:135345/140‑1 (MQ=255)                                                                                           tgttgtCCATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATggtggt                                          <  1:717366/140‑1 (MQ=255)                                                                                             ttgtCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCg                                        >  1:1041579/1‑140 (MQ=255)                                                                                             ttgtCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCg                                        >  1:840749/1‑140 (MQ=255)                                                                                                tCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCg                                     <  2:927721/140‑1 (MQ=255)                                                                                                 cTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGt                                    <  1:554697/140‑1 (MQ=255)                                                                                                    taCTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTggg                                 <  1:708301/140‑1 (MQ=255)                                                                                                     aCTTTACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTgggg                                >  1:617482/1‑140 (MQ=255)                                                                                                         tACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGc                                      <  1:515603/130‑1 (MQ=255)                                                                                                         tACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGc                                      >  2:515603/1‑130 (MQ=255)                                                                                                         tACACATAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTAtt                            >  2:696850/1‑140 (MQ=255)                                                                                                          acacATAAGGAAGAGGGGAATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTc                           >  2:290918/1‑140 (MQ=255)                                                                                                            acaTAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGc                                      >  1:959911/1‑127 (MQ=255)                                                                                                            acaTAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGt                                    >  2:523848/1‑129 (MQ=255)                                                                                                            acaTAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGt                                    <  1:523848/129‑1 (MQ=255)                                                                                                            acaTAAGGAAGAGCGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGc                                      <  2:959911/127‑1 (MQ=255)                                                                                                              aTAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTAtt                            >  2:44651/1‑135 (MQ=255)                                                                                                              aTAAGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTAtt                            <  1:44651/135‑1 (MQ=255)                                                                                                                aaGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCt                     >  1:20349/1‑140 (MQ=255)                                                                                                                 aGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTATTGGTGGTCGGCGTGGGGTATTCAATCCTc                    >  2:678962/1‑140 (MQ=255)                                                                                                                 aGGAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTc                    >  1:435357/1‑140 (MQ=255)                                                                                                                   gAAGAGGGGTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCaa                  <  2:1041579/140‑1 (MQ=255)                                                                                                                         gggTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGtt            >  2:67189/1‑140 (MQ=255)                                                                                                                          ggTATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTa           >  2:652267/1‑140 (MQ=255)                                                                                                                            tATTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTAcc         <  1:868883/140‑1 (MQ=255)                                                                                                                             aTTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACca        <  2:973054/140‑1 (MQ=255)                                                                                                                             aTTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACca        <  2:395728/140‑1 (MQ=255)                                                                                                                             aTTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACca        <  1:290918/140‑1 (MQ=255)                                                                                                                             aTTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACca        <  1:578675/140‑1 (MQ=255)                                                                                                                             aTTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACca        <  2:645619/140‑1 (MQ=255)                                                                                                                             aTTCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACca        >  1:643340/1‑140 (MQ=255)                                                                                                                              ttCCCTGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACcac       >  1:1054233/1‑140 (MQ=255)                                                                                                                                   tGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGttt  >  1:269820/1‑140 (MQ=255)                                                                                                                                   tGTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGttt  >  2:97226/1‑140 (MQ=255)                                                                                                                                    gTTGCAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACca        <  1:587061/133‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTT  >  NC_000913/1258629‑1258898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑