Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,289,676	A→G	A144A (GCA→GCG)	rssA →	putative patatin‑like phospholipase RssA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,289,676	0	A	G	100.0%	237.2 / NA	67	A144A (GCA→GCG)	rssA	putative patatin‑like phospholipase RssA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/32);  total (35/32)

GAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCACCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGACCTGCAGCACGATGCTCATTTGATGCAACA  >  NC_000913/1289551‑1289810                                                                                                                              |                                                                                                                                       gAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATaa                                                                                                                          <  1:431064/140‑1 (MQ=255)       tGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTa                                                                                                                    <  1:532296/140‑1 (MQ=255)         ttCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATaa                                                                                                                          >  2:197584/1‑132 (MQ=255)         ttCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATaa                                                                                                                          <  1:197584/132‑1 (MQ=255)           cccGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTActgg                                                                                                                >  2:465048/1‑140 (MQ=255)           cccGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTActgg                                                                                                                <  1:698218/140‑1 (MQ=255)             cGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTActggct                                                                                                              <  1:957410/140‑1 (MQ=255)              gTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTActggctg                                                                                                             >  1:435971/1‑140 (MQ=255)              gTCGCTTTGCGACTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTActggctg                                                                                                             >  2:230225/1‑140 (MQ=255)                         gCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGc                                                                                                  >  2:129809/1‑140 (MQ=255)                                        ttAAGTACGGGACGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAAt                                                                                   <  1:921070/140‑1 (MQ=255)                                                 ggACGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTc                                                                          >  2:772743/1‑140 (MQ=255)                                                  gACGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCaa                                                                                    <  2:577910/129‑1 (MQ=255)                                                  gACGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCaa                                                                                    >  1:577910/1‑129 (MQ=255)                                                  gACGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTcc                                                                         >  2:58712/1‑140 (MQ=255)                                                  gACGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTcc                                                                         >  2:696083/1‑140 (MQ=255)                                                    cGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCt                                                                       <  2:166674/140‑1 (MQ=255)                                                    cGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCt                                                                       <  2:435971/140‑1 (MQ=255)                                                    cGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCt                                                                       <  1:465048/140‑1 (MQ=255)                                                      tGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCa                                                                     >  2:96420/1‑140 (MQ=255)                                                       gAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCAc                                                                    <  1:818724/140‑1 (MQ=255)                                                              ggTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCa                                                             >  2:28826/1‑140 (MQ=255)                                                                tttACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCAtt                                                           <  2:151840/140‑1 (MQ=255)                                                                tttACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCAtt                                                           <  1:96420/140‑1 (MQ=255)                                                                tttACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCAtt                                                           <  1:129809/140‑1 (MQ=255)                                                                 ttACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTg                                                          <  1:468954/140‑1 (MQ=255)                                                                 ttACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTg                                                          <  1:58712/140‑1 (MQ=255)                                                                  tACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCAt                                                            <  1:486661/137‑1 (MQ=255)                                                                  tACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCAt                                                            >  2:486661/1‑137 (MQ=255)                                                                    cTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCGGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTgggg                                                       <  2:382867/140‑1 (MQ=255)                                                                      gAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTCCCCTCACGCGTGCATTGGGGGc                                                     <  1:558094/140‑1 (MQ=255)                                                                       aaGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCt                                                    <  1:383533/140‑1 (MQ=255)                                                                         ggCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTggggg                                                      >  1:633494/1‑136 (MQ=255)                                                                         ggCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTggggg                                                      <  2:633494/136‑1 (MQ=255)                                                                          gCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTggggg                                                      <  2:1019766/135‑1 (MQ=255)                                                                          gCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTggggg                                                      >  1:1019766/1‑135 (MQ=255)                                                                              tctcCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATtg                                             >  1:771113/1‑140 (MQ=255)                                                                              tctcCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATtg                                             >  1:556305/1‑140 (MQ=255)                                                                              tctcCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATtg                                             >  1:114613/1‑140 (MQ=255)                                                                              tctcCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATtg                                             >  2:26645/1‑140 (MQ=255)                                                                                 ccATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATTGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCt                                                    <  1:688055/130‑1 (MQ=255)                                                                                 ccATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATTGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCt                                                    >  2:688055/1‑130 (MQ=255)                                                                                    tCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAg                                       >  1:536413/1‑140 (MQ=255)                                                                                     cTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGc                                      >  2:317473/1‑140 (MQ=255)                                                                                         cTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGtt                                  >  1:1062727/1‑140 (MQ=255)                                                                                          tATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTg                                 <  1:230225/140‑1 (MQ=255)                                                                                          tATTCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTg                                 >  2:869845/1‑140 (MQ=255)                                                                                            ttCGCGCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGAc                               <  2:1062727/140‑1 (MQ=255)                                                                                              cgcgCATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGACCt                             >  1:582677/1‑140 (MQ=255)                                                                                                cgcATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGAc                               >  1:417399/1‑136 (MQ=255)                                                                                                cgcATCATGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGAc                               <  2:417399/136‑1 (MQ=255)                                                                                                    tcatGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGTTGATATTGTGATAGCGGTTGACCTgcagca                       <  1:316657/140‑1 (MQ=255)                                                                                                    tcatGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGACCTgcagca                       <  1:26645/140‑1 (MQ=255)                                                                                                       tGCAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAACAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGACCTGCAGCACGa                    >  1:684811/1‑140 (MQ=255)                                                                                                         cAGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGACCTGCAGCACGATg                  <  2:582677/140‑1 (MQ=255)                                                                                                          aGTATTCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGACCTGCAGCACGATGc                 <  2:462647/140‑1 (MQ=255)                                                                                                               tCCAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGACCTGCAGCACGATGCTCAtt            >  1:758686/1‑140 (MQ=255)                                                                                                                 cAGGACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGACCTGCAGCACGATGCTCATTTg          >  2:314152/1‑140 (MQ=255)                                                                                                                    gACTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGACCTGCAGCACGATGCTCATTTGATg       <  1:314152/140‑1 (MQ=255)                                                                                                                      cTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGACCTGCAGCACGATGCTCATTTGATGca     >  1:1000113/1‑140 (MQ=255)                                                                                                                      cTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGACCTGCAGCACGATGCTCATTTGATGca     >  1:426888/1‑140 (MQ=255)                                                                                                                      cTCATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGACCTGCAGCACGATGCTCATTTGATGca     >  2:140236/1‑140 (MQ=255)                                                                                                                        cATGGCGCCTGTTGCACATAACGGCTTCTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGACCTGCAGCACGATGCTCATTTGATGcaac   <  2:35076/140‑1 (MQ=255)                                                                                                                        cATGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGACCTGCAGCACGATGCTCATTTGATGcaac   >  1:160418/1‑140 (MQ=255)                                                                                                                         aTGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGACCTGCAGCACGATGCTCATTTGATGcaaca  >  2:264688/1‑140 (MQ=255)                                                                                                                         aTGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGACCTGCAGCACGATGCTCATTTGATGcaaca  >  1:595061/1‑140 (MQ=255)                                                                                                                         aTGGCGCCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGACCTGCAGCACGATGCTCATTTGATGcaaca  >  1:616072/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                       GAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTCGCGCATCATGCAGTATTCCAGGACTCATGGCACCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAACCCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGACCTGCAGCACGATGCTCATTTGATGCAACA  >  NC_000913/1289551‑1289810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑