Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,303,471	T→C	R191R (CGT→CGC)	oppB →	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,303,471	0	T	C	100.0%	185.9 / NA	52	R191R (CGT→CGC)	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/31);  total (21/31)

GCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATG  >  NC_000913/1303337‑1303606                                                                                                                                       |                                                                                                                                        gCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTAc                                                                                                                                    >  1:220760/1‑140 (MQ=255)   aCCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTAccc                                                                                                                                  <  2:256229/140‑1 (MQ=255)    ccATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCg                                                                                                                                 <  1:511593/140‑1 (MQ=255)     cATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGt                                                                                                                                <  2:778203/140‑1 (MQ=255)     cATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGt                                                                                                                                <  2:564422/140‑1 (MQ=255)     cATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGt                                                                                                                                <  2:334096/140‑1 (MQ=255)     cATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCCCCAGTATTGCGCGCATTACCCGt                                                                                                                                <  1:294257/140‑1 (MQ=255)           taGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGctct                                                                                                                          <  1:1022485/140‑1 (MQ=255)           taGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGctct                                                                                                                          <  1:776360/140‑1 (MQ=255)              tCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATg                                                                                                                       >  2:736598/1‑140 (MQ=255)               cATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTAt                                                                                                                        <  2:971452/138‑1 (MQ=255)               cATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTAt                                                                                                                        >  1:971452/1‑138 (MQ=255)               cATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGa                                                                                                                      >  1:529372/1‑140 (MQ=255)               cATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGa                                                                                                                      >  2:710962/1‑140 (MQ=255)                aTGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGAt                                                                                                                     <  2:529372/140‑1 (MQ=255)                aTGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGAt                                                                                                                     <  1:710962/140‑1 (MQ=255)                 tGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGAtt                                                                                                                    >  2:472520/1‑140 (MQ=255)                  gATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTg                                                                                                                   <  2:967267/140‑1 (MQ=255)                    tatTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGaa                                                                                                                 <  2:220760/140‑1 (MQ=255)                        tGCGATCATTTTGCATTGGCTGCCGGGCTGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGAt                                                                                                                     >  2:29955/1‑132 (MQ=255)                        tGCGATCATTTTGCATTGGCTGCCGGGCTGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGAt                                                                                                                     <  1:29955/132‑1 (MQ=255)                        tGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTAt                                                                                                             >  2:872564/1‑140 (MQ=255)                          cGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTa                                                                                                           <  2:592201/140‑1 (MQ=255)                            aTCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTaca                                                                                                         >  2:804577/1‑140 (MQ=255)                                       ttGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTa                                                                                              <  2:349909/140‑1 (MQ=255)                                       ttGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTa                                                                                              <  1:736598/140‑1 (MQ=255)                                               cGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAAt                                                                                                  <  1:216455/128‑1 (MQ=255)                                               cGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAAt                                                                                                  >  2:216455/1‑128 (MQ=255)                                                        gCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTTCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCg                                                                             <  1:914757/140‑1 (MQ=255)                                                        gCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCg                                                                             <  1:604583/140‑1 (MQ=255)                                                          tGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGaa                                                                           <  2:187885/140‑1 (MQ=255)                                                                   ggggCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTAcc                                                                  >  1:1018126/1‑140 (MQ=255)                                                                       cgcTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATg                                                              >  1:162873/1‑140 (MQ=255)                                                                                    gATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCAttt                                                 >  2:502563/1‑140 (MQ=255)                                                                                        ttGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACg                                             <  2:1018126/140‑1 (MQ=255)                                                                                                ggtggCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCAt                                     >  1:259557/1‑140 (MQ=255)                                                                                                ggtggCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCAt                                     >  2:556708/1‑140 (MQ=255)                                                                                                         gTCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTg                            <  2:87490/140‑1 (MQ=255)                                                                                                          tCACTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGc                           >  1:569198/1‑140 (MQ=255)                                                                                                            aCTCGCTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGctc                         >  2:301268/1‑140 (MQ=255)                                                                                                                 cTTATATCGCCAGTATTGCGCGCGTTACCCGTGGCTCTATGATTGAAGTAATACACTCCAATTTTATTCGTACTGCCCCGGATAAAGGGTTACCTATGCGGCGGATGATTTTACGTCACGCATTAATACATGCTCTGTTa                    >  2:717078/1‑140 (MQ=255)                                                                                                                 cTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCt                             <  1:156711/131‑1 (MQ=255)                                                                                                                 cTTATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCt                             >  2:156711/1‑131 (MQ=255)                                                                                                                  ttATATCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTAc                   <  1:502563/140‑1 (MQ=255)                                                                                                                       tCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTg              <  2:569198/140‑1 (MQ=255)                                                                                                                       tCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTg              <  2:16857/140‑1 (MQ=255)                                                                                                                       tCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTg              <  2:162873/140‑1 (MQ=255)                                                                                                                       tCGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTg              <  2:152509/140‑1 (MQ=255)                                                                                                                        cGCCAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGc             <  1:717078/140‑1 (MQ=255)                                                                                                                          ccAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGctc           >  2:309102/1‑140 (MQ=255)                                                                                                                          ccAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGAATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGctc           >  2:722879/1‑140 (MQ=255)                                                                                                                           cAGTATTGCGCGCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGctct          <  1:192464/140‑1 (MQ=255)                                                                                                                                   cgcgCATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATg  >  2:370755/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        GCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATG  >  NC_000913/1303337‑1303606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑