Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,321,873	C→T	E358E (GAG→GAA)	trpE ←	anthranilate synthase subunit TrpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,321,873	0	C	T	100.0%	186.3 / NA	53	E358E (GAG→GAA)	trpE	anthranilate synthase subunit TrpE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/24);  total (29/24)

ACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATA  >  NC_000913/1321736‑1322007                                                                                                                                          |                                                                                                                                       aCTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGttc                                                                                                                                      <  2:222950/140‑1 (MQ=255)      gcgAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTAt                                                                                                                                 <  1:1024901/140‑1 (MQ=255)                aGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACg                                                                                                                       >  1:101062/1‑140 (MQ=255)                aGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACg                                                                                                                       >  2:742888/1‑140 (MQ=255)                    gCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCAtt                                                                                                                   >  2:716960/1‑140 (MQ=255)                          caTAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGt                                                                                                             >  1:133418/1‑140 (MQ=255)                             aGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGc                                                                                                                      <  1:640642/128‑1 (MQ=255)                             aGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGc                                                                                                                      >  2:640642/1‑128 (MQ=255)                             aGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTc                                                                                                                 >  2:327560/1‑133 (MQ=255)                             aGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTc                                                                                                                 <  1:327560/133‑1 (MQ=255)                             aGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCa                                                                                                          >  2:272586/1‑140 (MQ=255)                                   aaCGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACgg                                                                                                    <  2:853351/140‑1 (MQ=255)                                    aCGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGc                                                                                                   >  1:213666/1‑140 (MQ=255)                                    aCGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGc                                                                                                   >  2:27622/1‑140 (MQ=255)                                     cGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCt                                                                                                  >  2:8906/1‑140 (MQ=255)                                       gTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGt                                                                                                >  1:378629/1‑140 (MQ=255)                                         cAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCg                                                                                              >  2:1034437/1‑140 (MQ=255)                                          aaCTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCga                                                                                             >  1:1076026/1‑140 (MQ=255)                                          aaCTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCga                                                                                             <  1:190667/140‑1 (MQ=255)                                            cTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCgaga                                                                                           <  1:335746/140‑1 (MQ=255)                                               tGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGAtct                                                                                        <  1:672538/140‑1 (MQ=255)                                                  tGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTg                                                                                     <  1:272586/140‑1 (MQ=255)                                                      aTCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCA‑CAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTg                                                                                     >  1:740568/1‑135 (MQ=255)                                                      aTCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCA‑CAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTg                                                                                     <  2:740568/135‑1 (MQ=255)                                                       tCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAg                                                                                <  1:27622/140‑1 (MQ=255)                                                           cGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGaa                                                                            >  1:212590/1‑140 (MQ=255)                                                              cGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCa                                                                         <  2:529941/140‑1 (MQ=255)                                                                tAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATc                                                                       >  2:590491/1‑140 (MQ=255)                                                                tAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATc                                                                       <  2:213666/140‑1 (MQ=255)                                                                    ggCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGgcg                                                                   <  1:716960/140‑1 (MQ=255)                                                                          cGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGcgacga                                                             <  1:590491/140‑1 (MQ=255)                                                                                  cAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTgg                                                     >  2:874003/1‑140 (MQ=255)                                                                                    aaTGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGc                                                   <  1:19861/140‑1 (MQ=255)                                                                                                aTCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCaa                                       >  1:744562/1‑140 (MQ=255)                                                                                                     tACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCggg                                  <  2:744562/140‑1 (MQ=255)                                                                                                       cGGGCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTa                                >  1:41802/1‑140 (MQ=255)                                                                                                         ggCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGa                              >  1:328776/1‑140 (MQ=255)                                                                                                         ggCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGa                              >  1:431705/1‑140 (MQ=255)                                                                                                          gCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGAt                             >  2:1051835/1‑140 (MQ=255)                                                                                                          gCGAGATCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGAt                             >  2:639769/1‑140 (MQ=255)                                                                                                             agaTCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATc                                     <  1:406927/129‑1 (MQ=255)                                                                                                             agaTCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATc                                     >  2:406927/1‑129 (MQ=255)                                                                                                             agaTCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGAtctc                          >  2:446787/1‑140 (MQ=255)                                                                                                               aTCAACCAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCaa                        >  2:440118/1‑140 (MQ=255)                                                                                                                    ccAGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTgg                   <  2:946184/140‑1 (MQ=255)                                                                                                                      aGCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTggcg                 <  2:133418/140‑1 (MQ=255)                                                                                                                       gCATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTggcgg                <  1:742888/140‑1 (MQ=255)                                                                                                                        cATCAGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTggcggc               >  2:389284/1‑140 (MQ=255)                                                                                                                            aGATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCtggt           <  2:263542/140‑1 (MQ=255)                                                                                                                             gATGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCtggtg          >  1:76840/1‑140 (MQ=255)                                                                                                                              aTGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCtggtgg         <  2:378629/140‑1 (MQ=255)                                                                                                                              aTGTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCtggtgg         <  2:328776/140‑1 (MQ=255)                                                                                                                                gTTCAGACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGca       <  1:440118/140‑1 (MQ=255)                                                                                                                                     gACAGTTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATa  >  1:832383/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       ACTACGCGAGAGACGAGGTGCATCACATAGGAATAACGGTCAACTTTGGTGAGATCGGCGACGTAGCGGCTGCCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATA  >  NC_000913/1321736‑1322007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑