Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,429,354	A→G	A102A (GCA→GCG)	stfR →	Rac prophage; putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,429,354	0	A	G	100.0%	142.4 / NA	41	A102A (GCA→GCG)	stfR	Rac prophage; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/20);  total (21/20)

CGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCG  >  NC_000913/1429225‑1429483                                                                                                                                  |                                                                                                                                  cGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGtt                                                                                                                         >  1:110264/1‑140 (MQ=255) cGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGtt                                                                                                                         >  2:427738/1‑140 (MQ=255)      ttCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGaa                                                                                                                    >  1:73202/1‑140 (MQ=255)      ttCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGaa                                                                                                                    >  2:1085458/1‑140 (MQ=255)      ttCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGaa                                                                                                                    >  1:523644/1‑140 (MQ=255)      ttCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGCCCGGAGGCGCTGCGCCGTTTTGaa                                                                                                                    >  2:253037/1‑140 (MQ=255)        cTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACt                                                                                                                  <  1:159279/140‑1 (MQ=255)        cTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACt                                                                                                                  <  1:616769/140‑1 (MQ=255)         tGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTg                                                                                                                 <  1:802104/140‑1 (MQ=255)           ttGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGAt                                                                                                               <  1:186567/140‑1 (MQ=255)            tggtggAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGCCCGGAGGCGCTGCGCCGTTTTGAACTGAtg                                                                                                              <  1:253037/140‑1 (MQ=255)                   aGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAag                                                                                                       <  1:430107/140‑1 (MQ=255)                             ccgTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGcgcg                                                                                             >  1:441349/1‑140 (MQ=255)                                 cacaTGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAAc                                                                                         >  1:831003/1‑140 (MQ=255)                                        cGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCcg                                                                                  <  1:430551/140‑1 (MQ=255)                                             ccATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTg                                                                             >  1:264549/1‑140 (MQ=255)                                                ttACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGca                                                                          >  2:1061674/1‑140 (MQ=255)                                                   ccGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAg                                                                       <  1:1085458/140‑1 (MQ=255)                                                     gtgtATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGaa                                                                     >  1:796619/1‑140 (MQ=255)                                                      tgtATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGc                                                                           <  1:645952/133‑1 (MQ=255)                                                      tgtATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGc                                                                           >  2:645952/1‑133 (MQ=255)                                                              gATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGc                                                            <  1:427738/140‑1 (MQ=255)                                                                ttCTCAACCCGGTACGCTGAATGATTTTCTCGGTACCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCcg                                                          >  2:411574/1‑140 (MQ=255)                                                                        ccGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGaagaag                                                  <  2:110264/140‑1 (MQ=255)                                                                         cGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGt                                                 >  2:811614/1‑140 (MQ=255)                                                                                 tGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGt                                         >  1:45/1‑140 (MQ=255)                                                                                    aTGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGAt                                      <  2:264549/140‑1 (MQ=255)                                                                                    aTGATTTTCTCGGTACCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGAt                                      <  1:411574/140‑1 (MQ=255)                                                                                      gATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGc                                    <  1:811614/140‑1 (MQ=255)                                                                                      gATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGc                                    <  2:831003/140‑1 (MQ=255)                                                                                              cGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACAt                            <  1:209723/140‑1 (MQ=255)                                                                                              cGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACAt                            <  2:45/140‑1 (MQ=255)                                                                                                 tGCCATGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAg                         >  1:1022382/1‑140 (MQ=255)                                                                                                      tGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGAt                                      <  1:519982/122‑1 (MQ=255)                                                                                                      tGACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGAt                                      >  2:519982/1‑122 (MQ=255)                                                                                                       gACGGAGGATGATGCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTg                   <  2:441349/140‑1 (MQ=255)                                                                                                           gaggaTGATGCCCGTCCGGAGGCGCTGCGCCTTTCTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAggc               <  1:1055381/140‑1 (MQ=255)                                                                                                                  atgCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCaa          <  2:790548/138‑1 (MQ=255)                                                                                                                  atgCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCaa          >  1:790548/1‑138 (MQ=255)                                                                                                                   tgCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAAccc       >  1:495325/1‑140 (MQ=255)                                                                                                                    gCCCGTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCa      >  2:606831/1‑140 (MQ=255)                                                                                                                        gTCCGGAGGCGCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCg  >  2:108580/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                  CGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCG  >  NC_000913/1429225‑1429483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑