Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,551,142 C→A L228M (CTG→ATG)  fdnH → formate dehydrogenase N subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,551,1420CA100.0% 147.1 / NA 42L228M (CTG→ATG) fdnHformate dehydrogenase N subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/16);  total (26/16)

TCCACTTCGGCACCAAGAAGGAGATGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGTTGAT  >  NC_000913/1551011‑1551278
                                                                                                                                   |                                                                                                                                        
tCCACTTCGGCACCAAGAAGGAGATGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCa                                                                                                                                      >  2:94175/1‑136 (MQ=255)
tCCACTTCGGCACCAAGAAGGAGATGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCa                                                                                                                                      <  1:94175/136‑1 (MQ=255)
tCCACTTCGGCACCAAGAAGGAGATGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCAc                                                                                                                                  <  1:445782/140‑1 (MQ=255)
     ttCGGCACCAAGAAGGAGATGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGa                                                                                                                             >  1:461775/1‑140 (MQ=255)
        ggCACCAAGAAGGAGATGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCa                                                                                                                          >  1:321824/1‑140 (MQ=255)
        ggCACCAAGAAGGAGATGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCa                                                                                                                          >  1:465364/1‑140 (MQ=255)
          cACCAAGAAGGAGATGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGc                                                                                                                        <  2:906234/140‑1 (MQ=255)
          cACCAAGAAGGAGATGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAA‑GGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGcc                                                                                                                       >  2:110413/1‑140 (MQ=255)
          cACCAAGAAGGAGATGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAA‑GGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGcc                                                                                                                       >  2:705821/1‑140 (MQ=255)
                      gaTGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAA‑GGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCa                                                                                                           <  1:110413/140‑1 (MQ=255)
                      gaTGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAA‑GGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCa                                                                                                           <  1:705821/140‑1 (MQ=255)
                       aTGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCa                                                                                                           >  2:208261/1‑140 (MQ=255)
                       aTGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCa                                                                                                           >  1:69133/1‑140 (MQ=255)
                         gCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACg                                                                                                         >  2:326107/1‑140 (MQ=255)
                           tGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGt                                                                                                       >  2:1068014/1‑140 (MQ=255)
                           tGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGt                                                                                                       <  2:461775/140‑1 (MQ=255)
                               gCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGc                                                                                                   >  2:346986/1‑140 (MQ=255)
                                          aGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCg                                                                                        <  1:208261/140‑1 (MQ=255)
                                             gcgTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAg                                                                                     >  1:152091/1‑140 (MQ=255)
                                                     aaaCTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATTGacac                                                                             <  2:69133/140‑1 (MQ=255)
                                                     aaaCTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGacac                                                                             <  2:321824/140‑1 (MQ=255)
                                                     aaaCTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGacac                                                                             <  2:465364/140‑1 (MQ=255)
                                                           aaaGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGt                                                                       <  2:152091/140‑1 (MQ=255)
                                                            aaGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTa                                                                      <  1:1056674/140‑1 (MQ=255)
                                                             aGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTaa                                                                     <  2:220065/140‑1 (MQ=255)
                                                             aGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTaa                                                                     >  1:193024/1‑140 (MQ=255)
                                                             aGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTaa                                                                     >  1:27892/1‑140 (MQ=255)
                                                                   tGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGCCtgt                                                               >  2:1042991/1‑140 (MQ=255)
                                                                              aTGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGcgcg                                                    >  1:56314/1‑140 (MQ=255)
                                                                              aTGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGcgcg                                                    >  1:306868/1‑140 (MQ=255)
                                                                                gCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGtt                                                  >  1:840011/1‑140 (MQ=255)
                                                                                gCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGtt                                                  >  2:655/1‑140 (MQ=255)
                                                                                        cTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGc                                          >  1:1053913/1‑140 (MQ=255)
                                                                                                 ggAAGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCgg                                 >  1:428881/1‑140 (MQ=255)
                                                                                                    aGGGGTCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCggccg                              >  2:629603/1‑138 (MQ=255)
                                                                                                         tCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCggctggct                           <  1:304358/138‑1 (MQ=255)
                                                                                                         tCGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCggctggct                           >  2:304358/1‑138 (MQ=255)
                                                                                                          cGGTGGTACGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTa                        <  2:306868/140‑1 (MQ=255)
                                                                                                                  cGCACGTTATGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACt                >  1:992918/1‑140 (MQ=255)
                                                                                                                          aTGTACGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCACTGGTAGCGGCTGGCTTTATTGCCACTTTTGCCgg        >  2:664774/1‑140 (MQ=255)
                                                                                                                               cGTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCACTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGTTGa   <  1:664774/140‑1 (MQ=255)
                                                                                                                                gTGATGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGTTGAt  >  2:799672/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                                        
TCCACTTCGGCACCAAGAAGGAGATGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGTTGAT  >  NC_000913/1551011‑1551278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑