Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,745,969	T→C	K53K (AAA→AAG)	ydhQ ←	adhesin‑like autotransporter YdhQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,745,969	0	T	C	100.0%	275.1 / NA	76	K53K (AAA→AAG)	ydhQ	adhesin‑like autotransporter YdhQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (50/26);  total (50/26)

CCGTAGCGAGCGTAGAGAGACTAATTGCGCCTCCCTCGGACTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTAACAATTTGCACGTTCCCGTCGCTCATC  >  NC_000913/1745836‑1746104                                                                                                                                      |                                                                                                                                        ccGTAGCGAGCGTAGAGAGACTAATTGCGCCTCCCTCGGACTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAAc                                                                                                                                   <  2:541300/140‑1 (MQ=255)    tAGCGAGCGTAGAGAGACTAATTGCGCCTCCCTCGGACTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAAccag                                                                                                                                >  2:783724/1‑140 (MQ=255)         agcgTAGAGAGACTAATTGCGCCTCCCTCGGACTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACcagccagc                                                                                                                           >  2:1016992/1‑140 (MQ=255)           cgTAGAGAGACTAATTGCGCCTCCCTCGGACTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGccc                                                                                                                         >  2:1053912/1‑140 (MQ=255)            gTAGAGAGACTAATTGCGCCTCCCTCGGACTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGcccc                                                                                                                        >  1:968039/1‑140 (MQ=255)                    aCTAATTGCGCCTCCCTCGGACTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCGTTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCa                                                                                                                >  2:256239/1‑140 (MQ=255)                    aCTAATTGCGCCTCCCTCGGACTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCa                                                                                                                >  1:422947/1‑140 (MQ=255)                     cTAATTGCGCCTCCCTCGGACTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCAc                                                                                                               >  1:322387/1‑140 (MQ=255)                        aTTGCGCCTCCCTCGGACTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGt                                                                                                            >  1:1008615/1‑140 (MQ=255)                        aTTGCGCCTCCCTCGGACTGAACAATATCTGTGGCAATGGCACCATCGCTGACCAATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGt                                                                                                            >  2:768300/1‑140 (MQ=255)                              cctccctcGGACTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTc                                                                                                      >  2:836154/1‑140 (MQ=255)                              cctccctcGGACTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTc                                                                                                      >  1:67487/1‑140 (MQ=255)                               ctccctcGGACTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCg                                                                                                     <  2:37994/140‑1 (MQ=255)                                  cctcGGACTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGct                                                                                                  >  2:83679/1‑140 (MQ=255)                                  cctcGGACTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGct                                                                                                  >  2:605524/1‑140 (MQ=255)                                        aCTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCg                                                                                            <  2:309949/140‑1 (MQ=255)                                         cTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCg                                                                                            <  2:813338/139‑1 (MQ=255)                                         cTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCg                                                                                            >  1:813338/1‑139 (MQ=255)                                         cTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGc                                                                                           <  2:322387/140‑1 (MQ=255)                                            aaCAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAg                                                                                        >  2:952257/1‑140 (MQ=255)                                            aaCAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAg                                                                                        >  1:817201/1‑140 (MQ=255)                                            aaCAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCA‑TTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGc                                                                                       >  2:390202/1‑140 (MQ=255)                                               aaTATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCtt                                                                                     <  1:1053912/140‑1 (MQ=255)                                                      gtgGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCg                                                                                            <  2:139356/126‑1 (MQ=255)                                                      gtgGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCg                                                                                            >  1:139356/1‑126 (MQ=255)                                                      gtgGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAAt                                                                              >  1:388113/1‑140 (MQ=255)                                                          cAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAAc                                                                          >  1:1011426/1‑140 (MQ=255)                                                          cAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAAc                                                                          >  1:47733/1‑140 (MQ=255)                                                           aaTGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACt                                                                         >  2:252509/1‑140 (MQ=255)                                                              ggCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGc                                                                      <  1:783724/140‑1 (MQ=255)                                                              ggCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGc                                                                      <  1:83679/140‑1 (MQ=255)                                                               gCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGcc                                                                     <  1:212721/140‑1 (MQ=255)                                                               gCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGcc                                                                     <  1:1016992/140‑1 (MQ=255)                                                                 aCCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCtt                                                                   <  2:388113/140‑1 (MQ=255)                                                                    aTCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCg                                                                       <  2:398302/133‑1 (MQ=255)                                                                    aTCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCg                                                                       >  1:398302/1‑133 (MQ=255)                                                                     tCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGc                                                                      >  2:570786/1‑133 (MQ=255)                                                                     tCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGc                                                                      <  1:570786/133‑1 (MQ=255)                                                                               aTAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTc                                                     >  2:949118/1‑140 (MQ=255)                                                                                tAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTcg                                                    >  2:714417/1‑140 (MQ=255)                                                                                              tACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCg                                      >  1:216629/1‑140 (MQ=255)                                                                                              tACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCg                                      <  2:499913/140‑1 (MQ=255)                                                                                               aCCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGc                                     <  1:768300/140‑1 (MQ=255)                                                                                               aCCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGc                                     <  1:952257/140‑1 (MQ=255)                                                                                                ccGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGcc                                    <  1:605524/140‑1 (MQ=255)                                                                                                   tACTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGt                                 >  2:329785/1‑140 (MQ=255)                                                                                                    aCTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGcc                                    >  2:535498/1‑136 (MQ=255)                                                                                                    aCTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGcc                                    <  1:535498/136‑1 (MQ=255)                                                                                                    aCTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCC‑TCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCt                               >  2:374894/1‑140 (MQ=255)                                                                                                     cTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGc                                     <  1:333529/134‑1 (MQ=255)                                                                                                     cTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGc                                     >  2:333529/1‑134 (MQ=255)                                                                                                     cTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCt                               >  2:700020/1‑140 (MQ=255)                                                                                                     cTGTGTGCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCt                               >  1:17254/1‑140 (MQ=255)                                                                                                       gtgtgCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCTCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGcc                                    >  2:1002983/1‑133 (MQ=255)                                                                                                       gtgtgCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATTACCTCGCCGGTCTTa                             >  1:477757/1‑140 (MQ=255)                                                                                                       gtgtgCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGcc                                    <  1:1002983/133‑1 (MQ=255)                                                                                                       gtgtgCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTa                             >  2:147828/1‑140 (MQ=255)                                                                                                       gtgtgCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTa                             >  1:30717/1‑140 (MQ=255)                                                                                                       gtgtgCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTa                             >  1:944009/1‑140 (MQ=255)                                                                                                       gtgtgCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTa                             >  1:94674/1‑140 (MQ=255)                                                                                                        tgtgCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTaa                            >  2:46775/1‑140 (MQ=255)                                                                                                        tgtgCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTaa                            <  2:422947/140‑1 (MQ=255)                                                                                                          tgCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTAACa                          >  1:218844/1‑140 (MQ=255)                                                                                                          tgCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTAACa                          >  2:622014/1‑140 (MQ=255)                                                                                                          tgCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTAACa                          >  2:685870/1‑140 (MQ=255)                                                                                                          tgCATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTAACa                          >  2:71430/1‑140 (MQ=255)                                                                                                            cATTTGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCC‑TCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTAACAAtt                       <  1:374894/140‑1 (MQ=255)                                                                                                                tGGCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTAACAATTTGc                    >  2:40397/1‑140 (MQ=255)                                                                                                                 ggCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATTACCTCGCCGGTCTTAACAATTTGCa                   <  2:477757/140‑1 (MQ=255)                                                                                                                 ggCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTAACAATTTGCa                   <  1:71430/140‑1 (MQ=255)                                                                                                                 ggCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTAACAATTTGCa                   <  1:622014/140‑1 (MQ=255)                                                                                                                  gCGATCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTAACAATTTGCAc                  >  1:220758/1‑140 (MQ=255)                                                                                                                      tCCCACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGATCTTAACAATTTGCACGTTc              >  2:1023573/1‑140 (MQ=255)                                                                                                                       cccACCGGTTGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTAACAATTTGCACGTTcc             >  1:247250/1‑140 (MQ=255)                                                                                                                                tGCCACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTGACAATTTGCACGTTCCCGTCGCtca    >  2:131341/1‑140 (MQ=255)                                                                                                                                  ccACCTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTAACAATTTGCACGTTCCCGTCGCtcatc  >  1:903896/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        CCGTAGCGAGCGTAGAGAGACTAATTGCGCCTCCCTCGGACTGAACAATATCTGTGGCAATGGCACCATCGCTGACCGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTAAGTTGCGTCGCGCCAATGACCTCGCCGGTCTTAACAATTTGCACGTTCCCGTCGCTCATC  >  NC_000913/1745836‑1746104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑