Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,782,378	C→A	A328A (GCC→GCA)	ydiS →	putative oxidoreductase with FAD/NAD(P)‑binding domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,782,378	0	C	A	100.0%	178.7 / NA	51	A328A (GCC→GCA)	ydiS	putative oxidoreductase with FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/24);  total (27/24)

CTGGCAATGGTGCCGCAACTGGTTAACGAGGGCGTGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCCACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGATATGCAGCATTTTCGCAAGATCCCGGCG  >  NC_000913/1782250‑1782501                                                                                                                                 |                                                                                                                            cTGGCAATGGTGCCGCAACTGGTTAACGAGGGCGTGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGAt                                                                                                                  <  2:414359/140‑1 (MQ=255)     cAATGGTGCCGCAACTGGTTAACGAGGGCGTGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATcgcc                                                                                                              <  2:778349/140‑1 (MQ=255)       aTGGTGCCGCAACTGGTTAACGAGGGCGTGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATcgccgc                                                                                                            >  2:995080/1‑140 (MQ=255)                  aCTGGTTAACGAGGGCGTGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAAcgcg                                                                                                 >  2:843449/1‑140 (MQ=255)                    tGGTTAACGAGGGCGTGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCa                                                                                                          <  1:217509/129‑1 (MQ=255)                    tGGTTAACGAGGGCGTGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCa                                                                                                          >  2:217509/1‑129 (MQ=255)                        tAACGAGGGCGTGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGAtt                                                                                           >  1:219136/1‑140 (MQ=255)                        tAACGAGGGCGTGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGAtt                                                                                           >  1:855765/1‑140 (MQ=255)                             aGGGCGTGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTcc                                                                                      <  1:995080/140‑1 (MQ=255)                                gCGTGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGcc                                                                                   >  1:795846/1‑140 (MQ=255)                                 cGTGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGcta                                                                                  >  1:867425/1‑138 (MQ=255)                                 cGTGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCca                                                                                  >  2:474041/1‑140 (MQ=255)                                   tgatgaTCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCcagc                                                                                >  2:852715/1‑140 (MQ=255)                                     atgatCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCcagcag                                                                              >  1:326093/1‑140 (MQ=255)                                          cGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTgg                                                                         <  2:494662/140‑1 (MQ=255)                                          cGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTgg                                                                         >  2:986504/1‑140 (MQ=255)                                          cGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTgg                                                                         >  2:529054/1‑140 (MQ=255)                                            ttGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCTAGCAGTCTGgcg                                                                       <  2:867425/140‑1 (MQ=255)                                                 gACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATa                                                                  <  1:660418/140‑1 (MQ=255)                                                 gACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATa                                                                  <  1:810941/140‑1 (MQ=255)                                                   cgccgcAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGgcg                                                                       >  1:683704/1‑133 (MQ=255)                                                   cgccgcAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGgcg                                                                       <  2:683704/133‑1 (MQ=255)                                                   cgccgcAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACa                                                                >  2:745563/1‑140 (MQ=255)                                                   cgccgcAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACa                                                                <  1:129575/140‑1 (MQ=255)                                                      cgcAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAAc                                                             <  1:745563/140‑1 (MQ=255)                                                         aGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTg                                                          >  2:815875/1‑140 (MQ=255)                                                                 gCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGaa                                                  <  2:68367/140‑1 (MQ=255)                                                                       aTTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGc                                            <  1:986504/140‑1 (MQ=255)                                                                           gggTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCg                                        <  1:744969/140‑1 (MQ=255)                                                                           gggTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCg                                        <  2:1031649/140‑1 (MQ=255)                                                                             gTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTc                                      <  1:628727/140‑1 (MQ=255)                                                                             gTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTc                                      <  1:529054/140‑1 (MQ=255)                                                                                 tACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGc                                  >  1:285299/1‑140 (MQ=255)                                                                                    ggTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTg                               <  2:326093/140‑1 (MQ=255)                                                                                      tCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGat                             >  1:932659/1‑140 (MQ=255)                                                                                        cgcgGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGatat                           >  2:280167/1‑140 (MQ=255)                                                                                          cgGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGATATgc                         >  1:1041492/1‑140 (MQ=255)                                                                                               tGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGATATGCAGCAt                    <  2:219136/140‑1 (MQ=255)                                                                                                    ttAGCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGATATGCAGCATTTTCg               <  2:285299/140‑1 (MQ=255)                                                                                                       gCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGATATGCAGCATTTTCGCaa            <  1:474041/140‑1 (MQ=255)                                                                                                       gCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGATATGCAGCATTTTCGCaa            >  2:635147/1‑140 (MQ=255)                                                                                                       gCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGATATGCAGCATTTTCGCaa            <  1:815875/140‑1 (MQ=255)                                                                                                       gCCATTGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGATATGCAGCATTTTCGCaa            >  1:729722/1‑140 (MQ=255)                                                                                                           ttGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGATATGCAGCATTTTCGCAAGATc        >  2:691420/1‑140 (MQ=255)                                                                                                            tGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGATATGCAGCATTTTCGCAAGATcc       >  1:607861/1‑140 (MQ=255)                                                                                                            tGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGATATGCAGCATTTTCGCAAGATcc       >  2:1069815/1‑140 (MQ=255)                                                                                                            tGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGATATGCAGCATTTTCGCAAGATcc       >  1:3806/1‑140 (MQ=255)                                                                                                            tGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGATATGCAGCATTTTCGCAAGATcc       >  1:214273/1‑140 (MQ=255)                                                                                                            tGCATCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGATATGCAGCATTTTCGCAAGATcc       >  1:205892/1‑140 (MQ=255)                                                                                                               aTCGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGATATGCAGCATTTTCGCAAGATCCCgg    <  1:1081270/140‑1 (MQ=255)                                                                                                                 cGGCTCAGGCTGCCGCAACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGATATGCAGCATTTTCGCAAGATCCCGgcg  <  1:280167/140‑1 (MQ=255)                                                                                                                             ccgcaACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGATATGCAGCATTTTCGCAAGATCCCg     <  2:65975/125‑1 (MQ=255)                                                                                                                             ccgcaACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGATATGCAGCATTTTCGCAAGATCCCg     >  1:65975/1‑125 (MQ=255)                                                                                                                                 |                                                                                                                            CTGGCAATGGTGCCGCAACTGGTTAACGAGGGCGTGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCCACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTCTCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGATATGCAGCATTTTCGCAAGATCCCGGCG  >  NC_000913/1782250‑1782501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑