Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,791,142	T→C	T35A (ACC→GCC)	ydiU ←	UPF0061 family protein YdiU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,791,142	0	T	C	100.0%	212.8 / NA	59	T35A (ACC→GCC)	ydiU	UPF0061 family protein YdiU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/31);  total (28/31)

CCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACGGTTACTC  >  NC_000913/1791007‑1791278                                                                                                                                        |                                                                                                                                         cccAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGcatta                                                                                                                                      >  1:609537/1‑140 (MQ=255)            aaCTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAg                                                                                                                           >  1:1069412/1‑140 (MQ=255)                gatgaCCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCgg                                                                                                                       >  1:413258/1‑140 (MQ=255)                   gaCCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCa                                                                                                                    <  1:739586/140‑1 (MQ=255)                   gaCCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCa                                                                                                                    <  1:951852/140‑1 (MQ=255)                   gaCCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCa                                                                                                                    <  2:413258/140‑1 (MQ=255)                   gaCCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCa                                                                                                                    <  1:361988/140‑1 (MQ=255)                         tGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTAttt                                                                                                              >  2:890536/1‑140 (MQ=255)                           tAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTaa                                                                                                            <  1:890536/140‑1 (MQ=255)                                 tGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGt                                                                                                      >  2:117741/1‑140 (MQ=255)                                   cgcCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAg                                                                                                    >  1:177465/1‑140 (MQ=255)                                   cgcCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAg                                                                                                    >  2:17030/1‑140 (MQ=255)                                   cgcCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAg                                                                                                    >  1:131508/1‑140 (MQ=255)                                   cgcCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAg                                                                                                    >  1:902815/1‑140 (MQ=255)                                    gcCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAgg                                                                                                   <  1:117741/140‑1 (MQ=255)                                          ggtgACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAg                                                                                             <  2:131508/140‑1 (MQ=255)                                          ggtgACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAg                                                                                             <  2:609537/140‑1 (MQ=255)                                            tgACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTg                                                                                           <  1:17030/140‑1 (MQ=255)                                            tgACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTg                                                                                           <  2:177465/140‑1 (MQ=255)                                                  aCCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGtat                                                                                     <  1:1034680/140‑1 (MQ=255)                                                   ccAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGtata                                                                                    >  1:618721/1‑140 (MQ=255)                                                      ggCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTg                                                                                           >  1:1059119/1‑130 (MQ=255)                                                      ggCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTg                                                                                           <  2:1059119/130‑1 (MQ=255)                                                        cAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGttt                                                                               >  1:775228/1‑140 (MQ=255)                                                          gTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGAAAAGTGCTGTATAGGTTTCt                                                                             >  1:996578/1‑140 (MQ=255)                                                           tAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAg                                                                                             <  2:336363/123‑1 (MQ=255)                                                           tAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAg                                                                                             >  1:336363/1‑123 (MQ=255)                                                           tAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTg                                                                            >  2:1077424/1‑140 (MQ=255)                                                           tAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTg                                                                            <  2:121504/140‑1 (MQ=255)                                                            aGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTgg                                                                           >  1:584619/1‑140 (MQ=255)                                                               gCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCaa                                                                        <  2:1069412/140‑1 (MQ=255)                                                                     ccgccCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATc                                                                  >  1:982189/1‑140 (MQ=255)                                                                        ccccAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATcgcg                                                               >  1:1024360/1‑140 (MQ=255)                                                                                acCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAATTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCggg                                                       <  1:384908/140‑1 (MQ=255)                                                                                acCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCggg                                                       >  1:897546/1‑140 (MQ=255)                                                                                  cTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTa                                                     >  2:522289/1‑140 (MQ=255)                                                                                   tGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTaa                                                    >  2:625027/1‑140 (MQ=255)                                                                                        cATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACaaaa                                               >  1:473918/1‑140 (MQ=255)                                                                                         aTTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAg                                              >  2:161873/1‑140 (MQ=255)                                                                                          tttttAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGa                                             >  1:38271/1‑140 (MQ=255)                                                                                          tttttAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGa                                             <  1:161873/140‑1 (MQ=255)                                                                                           ttttAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGAc                                            <  1:522289/140‑1 (MQ=255)                                                                                            tttAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACa                                           <  2:38271/140‑1 (MQ=255)                                                                                            tttAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACa                                           <  2:631648/140‑1 (MQ=255)                                                                                              tAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAgg                                         <  1:750441/140‑1 (MQ=255)                                                                                                 aCAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTc                                      <  1:625027/140‑1 (MQ=255)                                                                                                     cgacgaTGGAATACTCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAg                                  >  2:833900/1‑140 (MQ=255)                                                                                                                   tCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGa                    <  2:897546/140‑1 (MQ=255)                                                                                                                   tCAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGa                    <  1:789228/140‑1 (MQ=255)                                                                                                                    cAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGAt                   <  2:473918/140‑1 (MQ=255)                                                                                                                    cAGCGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGAt                   <  1:666391/140‑1 (MQ=255)                                                                                                                       cGTGTTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAgt                >  2:787052/1‑140 (MQ=255)                                                                                                                          gtTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTa             <  2:982189/140‑1 (MQ=255)                                                                                                                          gtTAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTa             <  1:138367/140‑1 (MQ=255)                                                                                                                            tAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGa           >  2:440533/1‑140 (MQ=255)                                                                                                                            tAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGa           >  2:193448/1‑140 (MQ=255)                                                                                                                            tAGCCAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGa           <  1:440533/140‑1 (MQ=255)                                                                                                                               ccAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACgg        >  2:143220/1‑140 (MQ=255)                                                                                                                               ccAGTTCGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACgg        <  1:727314/140‑1 (MQ=255)                                                                                                                                     cGGCATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACGGTTActc  <  2:680806/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACGGTTACTC  >  NC_000913/1791007‑1791278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑