Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,837,283	G→A	L24L (CTG→CTA)	ynjC →	putative ABC transporter membrane subunit YnjC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,837,283	0	G	A	100.0%	212.2 / NA	61	L24L (CTG→CTA)	ynjC	putative ABC transporter membrane subunit YnjC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/28);  total (33/28)

CGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTGATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATT  >  NC_000913/1837148‑1837417                                                                                                                                        |                                                                                                                                       cGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATcc                                                                                                                                    >  2:885114/1‑140 (MQ=255)   cAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCg                                                                                                                                  >  2:315075/1‑140 (MQ=255)           gccgccGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGctct                                                                                                                          <  1:1051624/140‑1 (MQ=255)           gccgccGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGctct                                                                                                                          <  1:607713/140‑1 (MQ=255)                         cTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACg                                                                                                                      <  2:81245/130‑1 (MQ=255)                         cTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACg                                                                                                                      >  1:81245/1‑130 (MQ=255)                             aCCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGc                                                                                                        >  1:692419/1‑140 (MQ=255)                                gcacgcaGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCttt                                                                                                     <  1:602723/140‑1 (MQ=255)                                 cacgcaGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCATCTCTCACg                                                                                                                      >  1:759971/1‑122 (MQ=255)                                 cacgcaGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACg                                                                                                                      <  2:759971/122‑1 (MQ=255)                                 cacgcaGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTg                                                                                                    >  1:919415/1‑140 (MQ=255)                                  acgcaGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGt                                                                                                   <  1:803796/140‑1 (MQ=255)                                  acgcaGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGt                                                                                                   >  2:487827/1‑140 (MQ=255)                                      aGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGtt                                                                                               <  2:688403/140‑1 (MQ=255)                                                aaTGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTgg                                                                                     <  2:919415/140‑1 (MQ=255)                                                 aTGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGc                                                                                    <  2:431999/140‑1 (MQ=255)                                                  tGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCa                                                                                   >  2:486868/1‑140 (MQ=255)                                                  tGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCa                                                                                   >  2:266987/1‑140 (MQ=255)                                                  tGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCa                                                                                   >  1:791730/1‑140 (MQ=255)                                                  tGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCa                                                                                   >  1:579000/1‑140 (MQ=255)                                                  tGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCa                                                                                   >  1:86041/1‑140 (MQ=255)                                                      cTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCg                                                                               >  1:43984/1‑140 (MQ=255)                                                       tGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGt                                                                              >  1:965452/1‑140 (MQ=255)                                                             aaGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTg                                                                        <  2:786236/140‑1 (MQ=255)                                                             aaGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTg                                                                        <  1:486868/140‑1 (MQ=255)                                                                 aTGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGa                                                                    >  1:135456/1‑140 (MQ=255)                                                                     cTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCca                                                                >  1:751746/1‑140 (MQ=255)                                                                     cTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCca                                                                <  2:86041/140‑1 (MQ=255)                                                                         acCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGt                                                            <  2:1040622/140‑1 (MQ=255)                                                                         acCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGt                                                            >  1:1040622/1‑140 (MQ=255)                                                                             ttACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTAcc                                                        <  2:965452/140‑1 (MQ=255)                                                                             ttACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTAcc                                                        >  1:483264/1‑140 (MQ=255)                                                                             ttACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTAcc                                                        >  1:369966/1‑140 (MQ=255)                                                                              tACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCca                                                                <  1:694780/131‑1 (MQ=255)                                                                              tACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCca                                                                >  2:750452/1‑131 (MQ=255)                                                                              tACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCca                                                                >  2:694780/1‑131 (MQ=255)                                                                              tACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCca                                                                <  1:750452/131‑1 (MQ=255)                                                                              tACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTAccc                                                       >  2:288475/1‑139 (MQ=255)                                                                                 ggTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAg                                                    <  2:579000/140‑1 (MQ=255)                                                                                   tACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGc                                                  >  1:181043/1‑140 (MQ=255)                                                                                     cGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCAt                                                <  2:483264/140‑1 (MQ=255)                                                                                      gCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGt                                                            >  2:337722/1‑127 (MQ=255)                                                                                      gCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGt                                                            <  1:337722/127‑1 (MQ=255)                                                                                       cATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTa                                              >  1:742962/1‑140 (MQ=255)                                                                                       cATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTa                                              >  1:871142/1‑140 (MQ=255)                                                                                       cATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTa                                              <  2:692419/140‑1 (MQ=255)                                                                                               tttttGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAAc                                      >  2:812869/1‑140 (MQ=255)                                                                                                 tttGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGc                                    >  2:831162/1‑140 (MQ=255)                                                                                                 tttGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGc                                    <  1:315075/140‑1 (MQ=255)                                                                                                   tGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTg                                  <  2:751746/140‑1 (MQ=255)                                                                                                       gtgGGCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGgtgt                              >  2:281090/1‑140 (MQ=255)                                                                                                         gggCGATGGTGGCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCg                            >  2:133231/1‑140 (MQ=255)                                                                                                                    gCGGTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATcgc                 >  1:49110/1‑140 (MQ=255)                                                                                                                      ggTGATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCgcgg               <  1:831162/140‑1 (MQ=255)                                                                                                                         gATTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGcggcgg            >  1:313328/1‑140 (MQ=255)                                                                                                                          aTTTATGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGt           <  2:43984/140‑1 (MQ=255)                                                                                                                              aTGCACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCggg       <  1:753382/140‑1 (MQ=255)                                                                                                                                 cACCGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCa    <  1:812869/140‑1 (MQ=255)                                                                                                                                   ccGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCAtt  <  2:369966/140‑1 (MQ=255)                                                                                                                                    cGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATcgcg                >  1:194702/1‑125 (MQ=255)                                                                                                                                    cGCTAATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATcgcg                <  2:194702/125‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       CGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGCTACACCGTTACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTGATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATT  >  NC_000913/1837148‑1837417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑