Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,845,694	A→G	C423R (TGT→CGT)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,845,694	0	A	G	100.0%	187.2 / NA	52	C423R (TGT→CGT)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/28);  total (24/28)

ACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCG  >  NC_000913/1845561‑1845827                                                                                                                                      |                                                                                                                                      accacAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCg                                                                                                                                 <  2:557364/140‑1 (MQ=255)       ggCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAacac                                                                                                                           <  1:745306/140‑1 (MQ=255)            tGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCgg                                                                                                                                <  2:361060/130‑1 (MQ=255)            tGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCgg                                                                                                                                >  1:361060/1‑130 (MQ=255)            tGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCAt                                                                                                                      <  2:1038897/140‑1 (MQ=255)            tGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCAt                                                                                                                      >  1:735150/1‑140 (MQ=255)            tGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCAt                                                                                                                      >  2:211697/1‑140 (MQ=255)            tGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCAt                                                                                                                      <  1:422958/140‑1 (MQ=255)            tGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCAt                                                                                                                      >  1:449341/1‑140 (MQ=255)             ggCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATc                                                                                                                     >  1:472688/1‑140 (MQ=255)              gCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATcc                                                                                                                    >  1:1009334/1‑140 (MQ=255)                  gCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGc                                                                                                                >  2:28894/1‑140 (MQ=255)                      tcgtTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGaa                                                                                                            >  1:598951/1‑140 (MQ=255)                          tttCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGc                                                                                                        <  2:598951/140‑1 (MQ=255)                           ttcttcATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCa                                                                                                       <  1:785793/140‑1 (MQ=255)                           ttcttcATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCa                                                                                                       <  2:1069817/140‑1 (MQ=255)                           ttcttcATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCa                                                                                                       <  1:667278/140‑1 (MQ=255)                            tcttcATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCAt                                                                                                      <  2:449341/140‑1 (MQ=255)                                cATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGa                                                                                                  <  1:719675/140‑1 (MQ=255)                                  tCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATa                                                                                                >  1:800822/1‑140 (MQ=255)                                     cgcTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCa                                                                                                       >  2:292019/1‑130 (MQ=255)                                     cgcTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCa                                                                                                       <  1:292019/130‑1 (MQ=255)                                        tctTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACg                                                                                          <  1:271945/140‑1 (MQ=255)                                          tttGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACggg                                                                                        <  1:680765/140‑1 (MQ=255)                                          tttGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACggg                                                                                        <  2:893510/140‑1 (MQ=255)                                               tgcCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATc                                                                                   <  2:800822/140‑1 (MQ=255)                                               tgcCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATc                                                                                   <  1:211697/140‑1 (MQ=255)                                               tgcCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATc                                                                                   <  1:28894/140‑1 (MQ=255)                                               tgcCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATc                                                                                   <  1:469154/140‑1 (MQ=255)                                                gcCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCa                                                                                  <  2:674589/140‑1 (MQ=255)                                                      cAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTtat                                                                            >  2:254238/1‑140 (MQ=255)                                                           tGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCa                                                                                  <  2:723800/129‑1 (MQ=255)                                                           tGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCa                                                                                  >  1:723800/1‑129 (MQ=255)                                                           tGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGAcc                                                                       >  1:279114/1‑140 (MQ=255)                                                           tGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGAcc                                                                       >  2:697010/1‑140 (MQ=255)                                                           tGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGAcc                                                                       >  2:605642/1‑140 (MQ=255)                                                           tGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGAcc                                                                       >  2:524102/1‑140 (MQ=255)                                                           tGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGAcc                                                                       >  2:510437/1‑140 (MQ=255)                                                           tGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGAcc                                                                       >  2:280050/1‑140 (MQ=255)                                                           tGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGAcc                                                                       >  1:770371/1‑140 (MQ=255)                                                            gcgcCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACct                                                                      <  1:605642/140‑1 (MQ=255)                                                                      gCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGtt                                                            <  1:963569/140‑1 (MQ=255)                                                                        ttCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTtct                                                          <  1:946805/140‑1 (MQ=255)                                                                                            tAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGaa                                      <  1:280050/140‑1 (MQ=255)                                                                                                  cAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGGCGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGt                                <  2:472688/140‑1 (MQ=255)                                                                                                   aaaTTTGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTg                               <  1:44959/140‑1 (MQ=255)                                                                                                       ttGCCTTTGGCAATGTCCAGTTCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGt                           <  1:456363/140‑1 (MQ=255)                                                                                                                           ttCGATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGCGTGGTgcg       >  2:213386/1‑140 (MQ=255)                                                                                                                              gATAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGCGTGGTGCGCAt    >  2:223527/1‑140 (MQ=255)                                                                                                                               aTAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGCGTGGTGCGCATc   >  1:649338/1‑140 (MQ=255)                                                                                                                               aTAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGCGTGGTGCGCATc   >  1:821412/1‑140 (MQ=255)                                                                                                                                tAACACGCTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCg  >  1:9335/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                      ACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCG  >  NC_000913/1845561‑1845827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑