Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	137,887	A→G	K269E (AAG→GAG)	cueO →	cuprous oxidase/multicopper oxidase with role in copper homeostasis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	137,887	0	A	G	100.0%	175.0 / NA	49	K269E (AAG→GAG)	cueO	cuprous oxidase/multicopper oxidase with role in copper homeostasis
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/25);  total (24/25)

ACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGAAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAGCCAGATGGGGATGGCGATTGCGCCGTTTGATAAGCCT  >  NC_000913/137751‑138021                                                                                                                                         |                                                                                                                                       acacGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAgg                                                                                                                                     >  2:144277/1‑140 (MQ=255)  cacGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGt                                                                                                                                    <  1:48768/140‑1 (MQ=255)   acGCTGCCCCGCGTGGTTGGCCGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTg                                                                                                                                   >  2:353860/1‑140 (MQ=255)         ccccGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGaa                                                                                                                             <  1:400884/140‑1 (MQ=255)             gcgTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGc                                                                                                                         <  2:803692/140‑1 (MQ=255)              cgTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGcc                                                                                                                        >  2:1067888/1‑140 (MQ=255)               gTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCg                                                                                                                       <  2:548995/140‑1 (MQ=255)                   ttGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGc                                                                                                                   >  1:1024343/1‑140 (MQ=255)                            ccTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCg                                                                                                          >  2:503518/1‑140 (MQ=255)                             cTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGa                                                                                                         <  1:353860/140‑1 (MQ=255)                                     gCTCAATGGCTGTAATGCCCGTTCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTg                                                                                                 <  1:144277/140‑1 (MQ=255)                                      cTCAATGGCTGTAATGCCCGTTCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGa                                                                                                <  1:503518/140‑1 (MQ=255)                                                         gTTCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAAc                                                                             <  2:262650/140‑1 (MQ=255)                                                         gTTCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAAc                                                                             <  1:210825/140‑1 (MQ=255)                                                          ttCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACg                                                                            <  1:609293/140‑1 (MQ=255)                                                           tCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCtggtgg                                                                                     >  1:1059039/1‑130 (MQ=255)                                                           tCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCtggtgg                                                                                     <  2:1059039/130‑1 (MQ=255)                                                           tCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCtggtg                                                                                      >  1:59342/1‑129 (MQ=255)                                                           tCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCtggtg                                                                                      <  2:59342/129‑1 (MQ=255)                                                           tCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCtgg                                                                                        >  1:658854/1‑127 (MQ=255)                                                           tCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCtgg                                                                                        <  2:658854/127‑1 (MQ=255)                                                           tCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCtgg                                                                                        <  2:211050/127‑1 (MQ=255)                                                           tCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCtgg                                                                                        >  1:211050/1‑127 (MQ=255)                                                           tCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGa                                                                           >  2:922748/1‑140 (MQ=255)                                                           tCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGGTTTGAAGTGCTGGTGGAGGTTAACGa                                                                           >  2:725194/1‑140 (MQ=255)                                                              cTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATaa                                                                        >  1:21337/1‑140 (MQ=255)                                                                 aaTTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACaa                                                                     <  2:1024343/140‑1 (MQ=255)                                                                 aaTTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACaa                                                                     <  1:439987/140‑1 (MQ=255)                                                                             aGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCt                                                         >  2:913095/1‑140 (MQ=255)                                                                                            cTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGt                                          <  2:681575/140‑1 (MQ=255)                                                                                                     aTTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAGCCAGAt                                 >  2:686660/1‑140 (MQ=255)                                                                                                       tGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAg                                       >  2:867484/1‑132 (MQ=255)                                                                                                       tGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAg                                       <  1:867484/132‑1 (MQ=255)                                                                                                       tGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAGCCAGATgg                               >  2:806647/1‑140 (MQ=255)                                                                                                       tGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAGCCAGATgg                               >  2:692021/1‑140 (MQ=255)                                                                                                       tGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAGCCAGATgg                               >  2:824934/1‑140 (MQ=255)                                                                                                       tGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAGCCAGATgg                               >  2:238574/1‑140 (MQ=255)                                                                                                         ccAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAGCCAGATgggg                             >  2:380880/1‑140 (MQ=255)                                                                                                         ccAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAGCCAGATgggg                             >  2:590625/1‑140 (MQ=255)                                                                                                         ccAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAGCCAGATgg                               <  1:380240/138‑1 (MQ=255)                                                                                                         ccAGCGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAGCCAGATgg                               >  2:380240/1‑138 (MQ=255)                                                                                                             cGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAGc                                      >  2:456196/1‑127 (MQ=255)                                                                                                             cGACGGTGGTCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAGc                                      <  1:456196/127‑1 (MQ=255)                                                                                                                     gtCTGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAGCCAGATGGGGATGGCGATTgcg                 <  2:802194/140‑1 (MQ=255)                                                                                                                        tGCTACCTGAACCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAGCCAGATGGGGATGGCGATTGCGCCg              <  1:692021/140‑1 (MQ=255)                                                                                                                                 aaCCAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAGCCAGATGGGGATGGCGATTGCGCCGTTTGATAAg     <  1:380880/140‑1 (MQ=255)                                                                                                                                   ccAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAGCCAGATGGGGATGGTGATTGCGCCGTTTGATAAGcc   <  1:953477/140‑1 (MQ=255)                                                                                                                                   ccAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAGCCAGATGGGGATGGCGATTGCGCCGTTTGATAAGcc   <  1:806647/140‑1 (MQ=255)                                                                                                                                    cAGTGGAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAGCCAGATGGGGATGGCGATTGCGCCGTTTGATAAGCCt  >  2:449914/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       ACACGCTGCCCCGCGTGGTTGGCTGCGCCTGCGTTTGCTCAATGGCTGTAATGCCCGTTCGCTCAATTTCGCCACCAGCGACAATCGCCCGCTGTATGTGATTGCCAGCGACGGTGGTCTGCTACCTGAACCAGTGAAGGTGAGCGAACTGCCGGTGCTGATGGGCGAGCGTTTTGAAGTGCTGGTGGAGGTTAACGATAACAAACCCTTTGACCTGGTGACGCTGCCGGTCAGCCAGATGGGGATGGCGATTGCGCCGTTTGATAAGCCT  >  NC_000913/137751‑138021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑